PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

29692

Common Name

SELMODRAFT_113487, SELMODRAFT_29692, WRKY_2, WRKY_25

Organism

Selaginella moellendorffii

Taxonomic ID

88036

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Lycopodiidae; Selaginellales; Selaginellaceae; Selaginella

Family

WRKY

Protein Properties

Length: 67aa MW: 7842.72 Da PI: 7.9299

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
29692	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	89.4	2.9e-28	4	64	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST...T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 dDg++WrKYGqK++ +s+fprsYYrCt++ gC+++k v++++++p++ ++tY geH ++ 29692 4 DDGFTWRKYGQKDILNSKFPRSYYRCTHQkelGCQATKYVQKCEDEPSMYQVTYIGEHSCQ 64 8*************************99999***********************997 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50811	20.151	1	62	IPR003657	WRKY domain
Gene3D	G3DSA:2.20.25.80	8.3E-28	2	65	IPR003657	WRKY domain
SuperFamily	SSF118290	1.23E-26	2	64	IPR003657	WRKY domain
SMART	SM00774	4.4E-38	3	65	IPR003657	WRKY domain
Pfam	PF03106	9.4E-28	4	64	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009862	Biological Process	systemic acquired resistance, salicylic acid mediated signaling pathway
GO:0009864	Biological Process	induced systemic resistance, jasmonic acid mediated signaling pathway
GO:0010200	Biological Process	response to chitin
GO:0045892	Biological Process	negative regulation of transcription, DNA-templated
GO:0050832	Biological Process	defense response to fungus
GO:1900056	Biological Process	negative regulation of leaf senescence
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 67 aa Download sequence Send to blast
APADDGFTWR KYGQKDILNS KFPRSYYRCT HQKELGCQAT KYVQKCEDEP SMYQVTYIGE 60 HSCQNAQ

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
6ir8_A	3e-23	4	64	9	69	OsWRKY45
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in senescence, biotic and abiotic stress responses by modulating various phytohormones signaling pathways (PubMed:14742872, PubMed:16623907, PubMed:17310369, PubMed:28576847). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Binds to the 5'-[CT]GACTTTT-3' motif in promoters of target genes to induce their expression (PubMed:24104863). Plays an important but not indispensable role in jasmonate and salicylic acid signaling (PubMed:18713432). Regulates positively the salicylic acid (SA)-mediated signal pathway, but negatively the jasmonic acid (JA)-mediated signal pathway, thus determining the balance between these mutually antagonistic pathways (PubMed:14742872, PubMed:16623907, PubMed:18713432, PubMed:28837631). Together with WRKY46, WRKY53 and WRKY54, prevents defense response to the necrotrophic pathogens P.carotovorum and B.cinerea, but promotes defense responses (including SA-induced pathogenesis-related (PR) genes expression) against biotrophic/hemibiotrophic SA-monitored pathogens (e.g. P.syringae, E.carotovora subsp. carotovora SCC3193 and E.cichoracearum), probably by regulating negatively the JA/ET and positively the SA signaling pathways (PubMed:28837631, PubMed:16623907, PubMed:22325892). Contributes to the suppression of jasmonic acid (MeJA)-induced expression of JA-responsive genes (e.g. PDF1.2) (PubMed:22325892, PubMed:16623907). Promotes susceptibility to JA-monitored pathogens (e.g. A.brassicicola), probably by facilitating SA-controlled suppression of JA-mediated defense. Represses the biosynthesis of the phytoalexin camalexin and indol-3-ylmethyl glucosinolate (IGS) (PubMed:16623907). Represses both SA and JA/ethylene (ET) mediated defense marker genes expression (PubMed:17310369). Negative regulator of SA biosynthesis (PubMed:28837631). Negative regulator of EDS1-dependent defense against E.amylovora (PubMed:22316300). Required for RPP4-mediated disease resistance and basal defense against H.parasitica, probably via late up-regulation (LURP) of resistance genes (e.g. CML10/CaBP22 and LURP1) (PubMed:17313163). Probably involved in defense responses toward insects (e.g. P.xylostella and B.brassicae) (PubMed:25339349). Together with WRKY54, negative regulator of developmental senescence, probably via the regulation of several senescence-associated markers genes (PubMed:17310369, PubMed:22268143). Together with WRKY46 and WRKY54, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression (PubMed:28576847). In collaboration with WRKY54, prevents stomatal closure and, consequently, osmotic stress tolerance (PubMed:23815736). Regulates rhizobacterium B.cereus AR156-induced systemic resistance (ISR) to P.syringae pv. tomato DC3000 (PubMed:26433201). {ECO:0000250\|UniProtKB:Q9SUP6, ECO:0000269\|PubMed:14742872, ECO:0000269\|PubMed:16623907, ECO:0000269\|PubMed:17310369, ECO:0000269\|PubMed:17313163, ECO:0000269\|PubMed:18713432, ECO:0000269\|PubMed:22268143, ECO:0000269\|PubMed:22316300, ECO:0000269\|PubMed:22325892, ECO:0000269\|PubMed:23815736, ECO:0000269\|PubMed:24104863, ECO:0000269\|PubMed:25339349, ECO:0000269\|PubMed:26433201, ECO:0000269\|PubMed:28576847, ECO:0000269\|PubMed:28837631, ECO:0000303\|PubMed:28837631}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00408	DAP	Transfer from AT3G56400	Download

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Regulated by MYB44 (PubMed:23067202, PubMed:23603962). Basal expression levels require the presence of endogeneous salicylic acid (SA) (PubMed:14742872, PubMed:17310369). Induced by reactive oxygen species (ROS) (PubMed:22268143). Early but transient accumulation after osmotic stress (e.g. polyethylene glycol, PEG) (PubMed:23815736). Induced by SA; early induction is NPR1-independent, but full-scale induction is NPR1-dependent (PubMed:14742872, PubMed:22325892, PubMed:22268143, PubMed:26433201). Up-regulated by benzothiadiazole (BTH) (PubMed:26433201). Repressed by jasmonic acid (MeJA) by both COI1-dependent and COI1-independent pathways (PubMed:14742872, PubMed:18713432). Triggered by the pathogenic compatible bacteria E.carotovora subsp. carotovora SCC3193 (PubMed:14742872). Induced by P.syringae pv. tomato DC3000 (PubMed:17965588, PubMed:22325892). Stimulated by ATX1 (PubMed:17965588). Up-regulated by E.amylovora (PubMed:22316300). Accumulates during leaf and flower senescence (PubMed:17310369). Induced expression upon simultaneous feeding by caterpillars (e.g. P.xylostella) and aphids (e.g. B.brassicae) at a low density, but lower levels in plants induced with both caterpillars and a high aphid density (PubMed:25339349). Responsive to rhizobacterium B.cereus AR156 in leaves (PubMed:26433201). {ECO:0000269\|PubMed:14742872, ECO:0000269\|PubMed:17310369, ECO:0000269\|PubMed:17965588, ECO:0000269\|PubMed:18713432, ECO:0000269\|PubMed:22268143, ECO:0000269\|PubMed:22316300, ECO:0000269\|PubMed:22325892, ECO:0000269\|PubMed:23067202, ECO:0000269\|PubMed:23603962, ECO:0000269\|PubMed:23815736, ECO:0000269\|PubMed:25339349, ECO:0000269\|PubMed:26433201}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_002961829.2	7e-43	probable WRKY transcription factor 31
Swissprot	Q9LY00	3e-25	WRK70_ARATH; Probable WRKY transcription factor 70
TrEMBL	D8QTC4	6e-44	D8QTC4_SELML; Uncharacterized protein WRKY_25 (Fragment)
STRING	EFJ18006	1e-44	(Selaginella moellendorffii)
STRING	EFJ37089	1e-44	(Selaginella moellendorffii)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Representative plant	OGRP14	17	875

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G56400.1	1e-27	WRKY DNA-binding protein 70

Link Out ? help Back to Top
Phytozome	29692
Entrez Gene	9629159 \| 9657340

Publications ? help Back to Top
Banks JA, et al. The Selaginella genome identifies genetic changes associated with the evolution of vascular plants. Science, 2011. 332(6032): p. 960-3 [PMID:21551031] Zhang X, et al. Arabidopsis cysteine-rich receptor-like kinase 45 positively regulates disease resistance to Pseudomonas syringae. Plant Physiol. Biochem., 2013. 73: p. 383-91 [PMID:24215930] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Brosché M, et al. Transcriptomics and functional genomics of ROS-induced cell death regulation by RADICAL-INDUCED CELL DEATH1. PLoS Genet., 2014. 10(2): p. e1004112 [PMID:24550736] Bao F, et al. Arabidopsis HSP90 protein modulates RPP4-mediated temperature-dependent cell death and defense responses. New Phytol., 2014. 202(4): p. 1320-34 [PMID:24611624] Wang F, et al. TaNAC1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to Pseudomonas syringae, and promotes lateral root development in transgenic Arabidopsis thaliana. Front Plant Sci, 2015. 6: p. 108 [PMID:25774162] Jiang CH, et al. Transcription factors WRKY70 and WRKY11 served as regulators in rhizobacterium Bacillus cereus AR156-induced systemic resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis. J. Exp. Bot., 2016. 67(1): p. 157-74 [PMID:26433201] Kaurilind E,Xu E,Brosché M A genetic framework for H2O2 induced cell death in Arabidopsis thaliana. BMC Genomics, 2015. 16: p. 837 [PMID:26493993] Rodriguez-Salus M, et al. The Synthetic Elicitor 2-(5-Bromo-2-Hydroxy-Phenyl)-Thiazolidine-4-Carboxylic Acid Links Plant Immunity to Hormesis. Plant Physiol., 2016. 170(1): p. 444-58 [PMID:26530314] Onkokesung N,Reichelt M,van Doorn A,Schuurink RC,Dicke M Differential Costs of Two Distinct Resistance Mechanisms Induced by Different Herbivore Species in Arabidopsis. Plant Physiol., 2016. 170(2): p. 891-906 [PMID:26603653] Chen J, et al. Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses. Plant Cell, 2017. 29(6): p. 1425-1439 [PMID:28576847] Li J,Zhong R,Palva ET WRKY70 and its homolog WRKY54 negatively modulate the cell wall-associated defenses to necrotrophic pathogens in Arabidopsis. PLoS ONE, 2017. 12(8): p. e0183731 [PMID:28837631] Zhou M, et al. WRKY70 prevents axenic activation of plant immunity by direct repression of SARD1. New Phytol., 2018. 217(2): p. 700-712 [PMID:29076543]