PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT1G25330.1

Common Name

BHLH75, CES, EN78, F4F7.28, HAF

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bHLH

Protein Properties

Length: 223aa MW: 25209.3 Da PI: 6.9554

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT1G25330.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	26.7	1e-08	114	161	4	55
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +h+ +Er RR++iN+++ L++l+P + k + a +L ++Y++sLq AT1G25330.1 114 SHSLAERVRREKINERLKCLQDLVPGC----YKAMGMAVMLDVIIDYVRSLQ 161 8*************************8....345888999999999999998 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF47459	1.57E-16	108	177	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	1.15E-11	108	165	No hit	No description
PROSITE profile	PS50888	15.605	110	160	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	2.8E-16	110	172	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	4.8E-6	114	161	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	5.1E-9	116	166	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:2000488	Biological Process	positive regulation of brassinosteroid biosynthetic process
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0046983	Molecular Function	protein dimerization activity

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0009009	anatomy	plant embryo
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009052	anatomy	flower pedicel
PO:0020094	anatomy	plant egg cell
PO:0025022	anatomy	collective leaf structure
PO:0001185	developmental stage	plant embryo globular stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 223 aa Download sequence Send to blast
MARFEPYNYN NGHDPFFAHI NQNPELINLD LPASTPSSFM LFSNGALVDA NHNNSHFFPN 60 LLHGNTRRKG NKEESGSKRR RKRSEEEEAM NGDETQKPKD VVHVRAKRGQ ATDSHSLAER 120 VRREKINERL KCLQDLVPGC YKAMGMAVML DVIIDYVRSL QNQIEFLSMK LSAASACYDL 180 NSLDIEPTDI FQGGNIHSAA EMERILRESV GTQPPNFSST LPF

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	66	82	RRKGNKEESGSKRRRKR

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.63980	0.0	flower

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	186478867	0.0
Genevisible	245640_at	0.0
Expression Atlas	AT1G25330	-
AtGenExpress	AT1G25330	-
ATTED-II	AT1G25330	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed in leaves, stems, and flowers. {ECO:0000269\|PubMed:12679534}.

Function -- GeneRIF ? help Back to Top
HALF FILLED (HAF) gene, a bHLH-encoding transcription factor, is required for reproductive tract development. HAF acts redundantly with two closely related genes, BRASSINOSTEROID ENHANCED EXPRESSION1 (BEE1) and BEE3. [HALF FILLED] [HAF] [PMID: 21693516]

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT1G25330.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Interaction ? help Back to Top
Source	Intact With
IntAct	Search A4D998

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT1G25330

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK175757	0.0	AK175757.1 Arabidopsis thaliana mRNA for putative bHLH transcription factor (bHLH075), complete cds, clone: RAFL22-31-M12.
GenBank	AY088885	0.0	AY088885.1 Arabidopsis thaliana clone 98716 mRNA, complete sequence.
GenBank	DQ446289	0.0	DQ446289.1 Arabidopsis thaliana clone pENTR221-At1g25330 basic helix-loop-helix family protein (At1g25330) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_564229.1	1e-167	basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Swissprot	A4D998	1e-168	BH075_ARATH; Transcription factor bHLH75
TrEMBL	A0A178W3K0	1e-165	A0A178W3K0_ARATH; HAF
STRING	AT1G25330.1	1e-167	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM14081	20	24
Representative plant	OGRP58	16	313

Link Out ? help Back to Top
Phytozome	AT1G25330.1
Entrez Gene	839117
iHOP	AT1G25330
wikigenes	AT1G25330

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Friedrichsen DM, et al. Three redundant brassinosteroid early response genes encode putative bHLH transcription factors required for normal growth. Genetics, 2002. 162(3): p. 1445-56 [PMID:12454087] Abe H, et al. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell, 2003. 15(1): p. 63-78 [PMID:12509522] Heim MA, et al. The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity. Mol. Biol. Evol., 2003. 20(5): p. 735-47 [PMID:12679534] Toledo-Ortiz G,Huq E,Quail PH The Arabidopsis basic/helix-loop-helix transcription factor family. Plant Cell, 2003. 15(8): p. 1749-70 [PMID:12897250] Bailey PC, et al. Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana. Plant Cell, 2003. 15(11): p. 2497-502 [PMID:14600211] Cao D,Cheng H,Wu W,Soo HM,Peng J Gibberellin mobilizes distinct DELLA-dependent transcriptomes to regulate seed germination and floral development in Arabidopsis. Plant Physiol., 2006. 142(2): p. 509-25 [PMID:16920880] Underwood BA,Vanderhaeghen R,Whitford R,Town CD,Hilson P Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations. Plant Biotechnol. J., 2006. 4(3): p. 317-24 [PMID:17147637] Gong W, et al. The development of protein microarrays and their applications in DNA-protein and protein-protein interaction analyses of Arabidopsis transcription factors. Mol Plant, 2008. 1(1): p. 27-41 [PMID:19802365] Wuest SE, et al. Arabidopsis female gametophyte gene expression map reveals similarities between plant and animal gametes. Curr. Biol., 2010. 20(6): p. 506-12 [PMID:20226671] Poppenberger B, et al. CESTA, a positive regulator of brassinosteroid biosynthesis. EMBO J., 2011. 30(6): p. 1149-61 [PMID:21336258] Crawford BC,Yanofsky MF HALF FILLED promotes reproductive tract development and fertilization efficiency in Arabidopsis thaliana. Development, 2011. 138(14): p. 2999-3009 [PMID:21693516] Meinke DW A survey of dominant mutations in Arabidopsis thaliana. Trends Plant Sci., 2013. 18(2): p. 84-91 [PMID:22995285] Khan M, et al. Interplay between phosphorylation and SUMOylation events determines CESTA protein fate in brassinosteroid signalling. Nat Commun, 2014. 5: p. 4687 [PMID:25134617] Eremina M, et al. Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants. Proc. Natl. Acad. Sci. U.S.A., 2016. 113(40): p. E5982-E5991 [PMID:27655893] Pfannebecker KC,Lange M,Rupp O,Becker A Seed Plant-Specific Gene Lineages Involved in Carpel Development. Mol. Biol. Evol., 2017. 34(4): p. 925-942 [PMID:28087776]