PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT1G35460.1

Common Name

BHLH80, EN71, F12A4.2, FBH1

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bHLH

Protein Properties

Length: 259aa MW: 28526.6 Da PI: 5.2259

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT1G35460.1	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	40.5	4.8e-13	193	238	6	55
HHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 6 nerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +++Er RR ri +++ +L+el+P+ +k + a++Le+AveY+k Lq AT1G35460.1 193 SIAERVRRTRISDRIRRLQELVPNM----DKQTNTADMLEEAVEYVKALQ 238 689*******************7....688***************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50888	14.837	187	237	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	1.31E-16	190	253	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	4.42E-12	191	242	No hit	No description
Gene3D	G3DSA:4.10.280.10	5.5E-16	192	253	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	6.9E-13	193	243	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	3.8E-10	193	238	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0042335	Biological Process	cuticle development
GO:0045944	Biological Process	positive regulation of transcription from RNA polymerase II promoter
GO:0005634	Cellular Component	nucleus
GO:0001046	Molecular Function	core promoter sequence-specific DNA binding
GO:0001228	Molecular Function	transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
GO:0005515	Molecular Function	protein binding
GO:0046983	Molecular Function	protein dimerization activity

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000293	anatomy	guard cell

Sequence ? help Back to Top
Protein Sequence Length: 259 aa Download sequence Send to blast
MQSTHISGGS SGGGGGGGGE VSRSGLSRIR SAPATWIETL LEEDEEEGLK PNLCLTELLT 60 GNNNSGGVIT SRDDSFEFLS SVEQGLYNHH QGGGFHRQNS SPADFLSGSG SGTDGYFSNF 120 GIPANYDYLS TNVDISPTKR SRDMETQFSS QLKEEQMSGG ISGMMDMNMD KIFEDSVPCR 180 VRAKRGCATH PRSIAERVRR TRISDRIRRL QELVPNMDKQ TNTADMLEEA VEYVKALQSQ 240 IQELTEQQKR CKCKPKEEQ

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.18017	0.0	flower

Expression -- Microarray ? help Back to Top
Source	ID	E-value
GEO	30693194	0.0
Genevisible	252798_at	2e-34
Genevisible	260079_s_at	2e-34
Expression Atlas	AT1G35460	-
AtGenExpress	AT1G35460	-

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, stems, and flowers. {ECO:0000269\|PubMed:12679534}.

Function -- GeneRIF ? help Back to Top
There are four CONSTANS transcriptional activators FBH1-4 which are related basic helix-loop-helix-type transcription factors that preferentially bind to the E-box cis-elements in the CO promoter. Overexpression of all FBH genes drastically elevated CO levels and caused early flowering regardless of photoperiod. [FBH1] [PMID: 22334645] Results indicate a role for FLOWERING BASIC HELIX-LOOP-HELIX 1 (FBH1, AT1G35460) as a transcriptional modulator of warm temperature signals and clock responses. [PMID: 25246594]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00181	DAP	27203113	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT1G35460.1

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT1G35460

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AF411791	0.0	AF411791.1 Arabidopsis thaliana At1g35460/F12A4_2 mRNA, complete cds.
GenBank	AY093794	0.0	AY093794.1 Arabidopsis thaliana At1g35460/F12A4_2 mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_174776.1	0.0	basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Swissprot	Q9C8P8	0.0	BH080_ARATH; Transcription factor bHLH80
TrEMBL	A0A178WC48	0.0	A0A178WC48_ARATH; FBH1
STRING	AT1G35460.1	0.0	(Arabidopsis thaliana)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM5104	25	51
Representative plant	OGRP511	15	80

Link Out ? help Back to Top
Phytozome	AT1G35460.1
Entrez Gene	840438
iHOP	AT1G35460
wikigenes	AT1G35460

Publications ? help Back to Top
Riechmann JL, et al. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000. 290(5499): p. 2105-10 [PMID:11118137] Heim MA, et al. The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity. Mol. Biol. Evol., 2003. 20(5): p. 735-47 [PMID:12679534] Toledo-Ortiz G,Huq E,Quail PH The Arabidopsis basic/helix-loop-helix transcription factor family. Plant Cell, 2003. 15(8): p. 1749-70 [PMID:12897250] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Bailey PC, et al. Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana. Plant Cell, 2003. 15(11): p. 2497-502 [PMID:14600211] Nawy T, et al. Transcriptional profile of the Arabidopsis root quiescent center. Plant Cell, 2005. 17(7): p. 1908-25 [PMID:15937229] Kuusk S,Sohlberg JJ,Magnus Eklund D,Sundberg E Functionally redundant SHI family genes regulate Arabidopsis gynoecium development in a dose-dependent manner. Plant J., 2006. 47(1): p. 99-111 [PMID:16740146] Panjabi P, et al. Comparative mapping of Brassica juncea and Arabidopsis thaliana using Intron Polymorphism (IP) markers: homoeologous relationships, diversification and evolution of the A, B and C Brassica genomes. BMC Genomics, 2008. 9: p. 113 [PMID:18315867] Elrouby N,Coupland G Proteome-wide screens for small ubiquitin-like modifier (SUMO) substrates identify Arabidopsis proteins implicated in diverse biological processes. Proc. Natl. Acad. Sci. U.S.A., 2010. 107(40): p. 17415-20 [PMID:20855607] Ito S, et al. FLOWERING BHLH transcriptional activators control expression of the photoperiodic flowering regulator CONSTANS in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A., 2012. 109(9): p. 3582-7 [PMID:22334645] Efroni I, et al. Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses. Dev. Cell, 2013. 24(4): p. 438-45 [PMID:23449474] Nagel DH,Pruneda-Paz JL,Kay SA FBH1 affects warm temperature responses in the Arabidopsis circadian clock. Proc. Natl. Acad. Sci. U.S.A., 2014. 111(40): p. 14595-600 [PMID:25246594] Li S, et al. CFLAP1 and CFLAP2 Are Two bHLH Transcription Factors Participating in Synergistic Regulation of AtCFL1-Mediated Cuticle Development in Arabidopsis. PLoS Genet., 2016. 12(1): p. e1005744 [PMID:26745719]