PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

AT3G06590.2

Common Name

AIF2, BHLH148, EN143, F5E6.8

Organism

Arabidopsis thaliana

Taxonomic ID

3702

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis

Family

bHLH

Protein Properties

Length: 221aa MW: 24256 Da PI: 12.3375

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
AT3G06590.2	genome	TAIR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	14.4	7.1e-05	164	198	16	55
HHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 16 iNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +N + L +l+P + +K s iLe+A++YI+ L+ AT3G06590.2 164 VNRKVRVLGRLVPGC-----GKQSVPVILEEATDYIQALE 198 566667778888888.....9****************985 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF47459	6.19E-9	145	213	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS50888	11.077	148	197	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	8.0E-7	163	207	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0005634	Cellular Component	nucleus
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0005515	Molecular Function	protein binding
GO:0046983	Molecular Function	protein dimerization activity

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0000013	anatomy	cauline leaf
PO:0000037	anatomy	shoot apex
PO:0000230	anatomy	inflorescence meristem
PO:0000293	anatomy	guard cell
PO:0008019	anatomy	leaf lamina base
PO:0009005	anatomy	root
PO:0009006	anatomy	shoot system
PO:0009009	anatomy	plant embryo
PO:0009010	anatomy	seed
PO:0009025	anatomy	vascular leaf
PO:0009029	anatomy	stamen
PO:0009030	anatomy	carpel
PO:0009031	anatomy	sepal
PO:0009032	anatomy	petal
PO:0009046	anatomy	flower
PO:0009047	anatomy	stem
PO:0009052	anatomy	flower pedicel
PO:0020030	anatomy	cotyledon
PO:0020038	anatomy	petiole
PO:0020100	anatomy	hypocotyl
PO:0020137	anatomy	leaf apex
PO:0025022	anatomy	collective leaf structure
PO:0025281	anatomy	pollen
PO:0001054	developmental stage	vascular leaf senescent stage
PO:0001078	developmental stage	plant embryo cotyledonary stage
PO:0001081	developmental stage	mature plant embryo stage
PO:0001185	developmental stage	plant embryo globular stage
PO:0004507	developmental stage	plant embryo bilateral stage
PO:0007064	developmental stage	LP.12 twelve leaves visible stage
PO:0007095	developmental stage	LP.08 eight leaves visible stage
PO:0007098	developmental stage	LP.02 two leaves visible stage
PO:0007103	developmental stage	LP.10 ten leaves visible stage
PO:0007115	developmental stage	LP.04 four leaves visible stage
PO:0007123	developmental stage	LP.06 six leaves visible stage
PO:0007611	developmental stage	petal differentiation and expansion stage
PO:0007616	developmental stage	flowering stage

Sequence ? help Back to Top
Protein Sequence Length: 221 aa Download sequence Send to blast
MASLISDIEP PTSTTSDLVR RKKRSSASSA ASSRSSASSV SGEIHARWRS EKQQRIYSAK 60 LFQALQQVRL NSSASTSSSP TAQKRGKAVR EAADRALAVS ARGRTLWSRA ILANRIKLKF 120 RKQRRPRATM AIPAMTTVVS SSSNRSRKRR VSVLRLNKKS IPDVNRKVRV LGRLVPGCGK 180 QSVPVILEEA TDYIQALEMQ VRAMNSLVQL LSSYGSAPPP I

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
At.25233	0.0	flower\| root\| silique

Expression -- Microarray ? help Back to Top
Source	ID	E-value
Genevisible	258511_at	0.0
Expression Atlas	AT3G06590	-
AtGenExpress	AT3G06590	-
ATTED-II	AT3G06590	-

Functional Description ? help Back to Top
Source	Description
UniProt	bHLH transcription factor that binds DNA on specific sequence 5'-CANNTG-3' in target gene promoters (PubMed:24009530). Negatively regulates brassinosteroid signaling (PubMed:20023194). Together with BHLH148/RITF1, regulates the transcription of several genes involved in the detoxification of reactive oxygen species (ROS) generated by salt (NaCl) stress. Confers tolerance to salt and to the oxidative stress-inducing reagents hydrogen peroxide H(2)O(2) and methyl viologen (MV) (PubMed:24009530). {ECO:0000269\|PubMed:20023194, ECO:0000269\|PubMed:24009530}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	AT3G06590.2

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Slightly induced by salt (NaCl) stress. {ECO:0000269\|PubMed:24009530}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Interaction ? help Back to Top
Source	Intact With
BioGRID	AT4G34530, AT4G36540, AT5G15160, AT1G59640
IntAct	Search Q9C8Z9

Phenotype -- Mutation ? help Back to Top
Source	ID
T-DNA Express	AT3G06590

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AC020580	0.0	AC020580.11 Arabidopsis thaliana chromosome 3 BAC F5E6 genomic sequence, complete sequence.
GenBank	AY081288	0.0	AY081288.1 Arabidopsis thaliana unknown protein (At3g06590) mRNA, complete cds.
GenBank	AY093390	0.0	AY093390.1 Arabidopsis thaliana unknown protein (At3g06590) mRNA, complete cds.
GenBank	CP002686	0.0	CP002686.1 Arabidopsis thaliana chromosome 3, complete sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_001325894.1	1e-154	basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Refseq	NP_001325895.1	1e-154	basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Refseq	NP_566287.1	1e-154	basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Refseq	NP_974239.1	1e-154	basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Swissprot	Q9C8Z9	1e-156	BH148_ARATH; Transcription factor bHLH148
TrEMBL	A0A178VAS2	1e-152	A0A178VAS2_ARATH; AIF2
STRING	AT3G06590.2	1e-154	(Arabidopsis thaliana)

Link Out ? help Back to Top
Phytozome	AT3G06590.2
Entrez Gene	819838
iHOP	AT3G06590
wikigenes	AT3G06590

Publications ? help Back to Top
Heim MA, et al. The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity. Mol. Biol. Evol., 2003. 20(5): p. 735-47 [PMID:12679534] Toledo-Ortiz G,Huq E,Quail PH The Arabidopsis basic/helix-loop-helix transcription factor family. Plant Cell, 2003. 15(8): p. 1749-70 [PMID:12897250] Dal Bosco C, et al. Inactivation of the chloroplast ATP synthase gamma subunit results in high non-photochemical fluorescence quenching and altered nuclear gene expression in Arabidopsis thaliana. J. Biol. Chem., 2004. 279(2): p. 1060-9 [PMID:14576160] Yamada K, et al. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science, 2003. 302(5646): p. 842-6 [PMID:14593172] Bailey PC, et al. Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana. Plant Cell, 2003. 15(11): p. 2497-502 [PMID:14600211] Fizames C, et al. The Arabidopsis root transcriptome by serial analysis of gene expression. Gene identification using the genome sequence. Plant Physiol., 2004. 134(1): p. 67-80 [PMID:14730065] Wagner R,Pfannschmidt T Eukaryotic transcription factors in plastids--Bioinformatic assessment and implications for the evolution of gene expression machineries in plants. Gene, 2006. 381: p. 62-70 [PMID:16934950] Wang H, et al. Regulation of Arabidopsis brassinosteroid signaling by atypical basic helix-loop-helix proteins. Plant Cell, 2009. 21(12): p. 3781-91 [PMID:20023194] Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Arabidopsis Interactome Mapping Consortium Evidence for network evolution in an Arabidopsis interactome map. Science, 2011. 333(6042): p. 601-7 [PMID:21798944] Ikeda M,Mitsuda N,Ohme-Takagi M ATBS1 INTERACTING FACTORs negatively regulate Arabidopsis cell elongation in the triantagonistic bHLH system. Plant Signal Behav, 2013. 8(3): p. e23448 [PMID:23333962] Efroni I, et al. Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses. Dev. Cell, 2013. 24(4): p. 438-45 [PMID:23449474] Guan Q,Wu J,Yue X,Zhang Y,Zhu J A nuclear calcium-sensing pathway is critical for gene regulation and salt stress tolerance in Arabidopsis. PLoS Genet., 2013. 9(8): p. e1003755 [PMID:24009530] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Sukweenadhi J, et al. Paenibacillus yonginensis DCY84(T) induces changes in Arabidopsis thaliana gene expression against aluminum, drought, and salt stress. Microbiol. Res., 2015. 172: p. 7-15 [PMID:25721473]