PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Cucsa.078810.1

Common Name

Csa_7G328830, WRKY50

Organism

Cucumis sativus

Taxonomic ID

3659

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis

Family

WRKY

Protein Properties

Length: 297aa MW: 33101.2 Da PI: 5.8169

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Cucsa.078810.1	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	88.9	4.3e-28	102	161	3	59
-SS-EEEEEEE--TT-SS-EEEEEE-ST...T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 3 DgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 Dg++WrKYGqK++ gs+fprsY+rC+++ gC ++k+v++s++dp++ e+tY+g+H+++ Cucsa.078810.1 102 DGFSWRKYGQKDILGSKFPRSYFRCSHRftqGCLATKQVQKSDNDPTIYEVTYKGRHTCN 161 9************************9999*************************97 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.1E-27	91	162	IPR003657	WRKY domain
PROSITE profile	PS50811	21.574	95	163	IPR003657	WRKY domain
SMART	SM00774	1.7E-38	100	162	IPR003657	WRKY domain
SuperFamily	SSF118290	1.31E-24	101	161	IPR003657	WRKY domain
Pfam	PF03106	5.0E-26	102	160	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0010200	Biological Process	response to chitin
GO:0048527	Biological Process	lateral root development
GO:0005730	Cellular Component	nucleolus
GO:0000987	Molecular Function	core promoter proximal region sequence-specific DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 297 aa Download sequence Send to blast
MEFLSLIQEL NQGKQLANQL RTHLHPSSSS HGILLIDKIL RSYENALLAL SGGAAASVNS 60 AIAPVNAAVQ DGDVSKKRKV MAKWSEQVKV SSASAVEGPG CDGFSWRKYG QKDILGSKFP 120 RSYFRCSHRF TQGCLATKQV QKSDNDPTIY EVTYKGRHTC NKALHSTNTP QEHQNTFLQH 180 PIPPKQEDKP LQQLHDPLCF MFSSDPIRVK SEDLEYANGG LFQPLRTPSP MFGSEVQDDL 240 SPFRESECSP TFESNDMFGL WCDFETEFVS IPSSMTNISI GDLEECFSFD NLEMFC*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
6ir8_A	9e-21	102	160	10	68	OsWRKY45
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in the regulation of osmotic stress responses and stomatal movement (PubMed:24773321). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Positive regulator of EDS1-dependent defense against E.amylovora (PubMed:22316300). Together with WRKY70 and WRKY53, promotes resistance to P.syringae, probably by enhancing salicylic acid (SA)- dependent genes. Contributes to the suppression of jasmonic acid (MeJA)-induced expression of PDF1.2 (PubMed:22325892). Together with WRKY54 and WRKY70, promotes brassinosteroid (BR)-regulated plant growth but prevent drought response by modulating gene expression (PubMed:28576847). {ECO:0000250, ECO:0000269\|PubMed:22316300, ECO:0000269\|PubMed:22325892, ECO:0000269\|PubMed:24773321, ECO:0000269\|PubMed:28576847}.

Function -- GeneRIF ? help Back to Top
mediates defense responses to Pseudoperonospora cubensis infection [PMID: 30709494]

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00112	ampDAP	Transfer from AT2G46400	Download

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Up-regulated by drought, salt and hydrogen peroxide treatments (PubMed:24773321). Induced by salicylic acid (SA) (PubMed:22268143, PubMed:22325892). Up-regulated by E.amylovora (PubMed:22316300). Triggered by P.syringae (PubMed:22325892). {ECO:0000269\|PubMed:22268143, ECO:0000269\|PubMed:22316300, ECO:0000269\|PubMed:22325892, ECO:0000269\|PubMed:24773321}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	GU984035	0.0	GU984035.1 Cucumis sativus WRKY protein (WRKY50) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_001267693.1	0.0	probable WRKY transcription factor 46-like
Swissprot	Q9SKD9	2e-41	WRK46_ARATH; Probable WRKY transcription factor 46
TrEMBL	E7CEY4	0.0	E7CEY4_CUCSA; WRKY protein
STRING	XP_004164459.1	0.0	(Cucumis sativus)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF24713	3	3

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G23810.1	3e-43	WRKY family protein

Link Out ? help Back to Top
Phytozome	Cucsa.078810.1
Entrez Gene	101209522

Publications ? help Back to Top
Ren Y, et al. An integrated genetic and cytogenetic map of the cucumber genome. PLoS ONE, 2009. 4(6): p. e5795 [PMID:19495411] Guo S, et al. Transcriptome sequencing and comparative analysis of cucumber flowers with different sex types. BMC Genomics, 2010. 11: p. 384 [PMID:20565788] Ling J, et al. Genome-wide analysis of WRKY gene family in Cucumis sativus. BMC Genomics, 2011. 12: p. 471 [PMID:21955985] Li Z, et al. RNA-Seq improves annotation of protein-coding genes in the cucumber genome. BMC Genomics, 2011. 12: p. 540 [PMID:22047402] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Sheikh AH, et al. Regulation of WRKY46 Transcription Factor Function by Mitogen-Activated Protein Kinases in Arabidopsis thaliana. Front Plant Sci, 2016. 7: p. 61 [PMID:26870073] Yan JY, et al. A WRKY Transcription Factor Regulates Fe Translocation under Fe Deficiency. Plant Physiol., 2016. 171(3): p. 2017-27 [PMID:27208259] Chen J, et al. Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses. Plant Cell, 2017. 29(6): p. 1425-1439 [PMID:28576847] Ma J, et al. Overexpression of TaWRKY146 Increases Drought Tolerance through Inducing Stomatal Closure in Arabidopsis thaliana. Front Plant Sci, 2017. 8: p. 2036 [PMID:29225611] Luan Q, et al. CsWRKY50 mediates defense responses to Pseudoperonospora cubensis infection in Cucumis sativus. Plant Sci., 2019. 279: p. 59-69 [PMID:30709494]