PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Cucsa.282040.1

Common Name

WRKY5

Organism

Cucumis sativus

Taxonomic ID

3659

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis

Family

WRKY

Protein Properties

Length: 283aa MW: 30971.1 Da PI: 10.2108

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Cucsa.282040.1	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	99.1	2.7e-31	212	270	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 +D+y+WrKYGqK++kgs++pr+YYrC+s gCp++kkver+++dp ++ +tYeg+H+h+ Cucsa.282040.1 212 SDEYSWRKYGQKPIKGSPYPRGYYRCSSVkGCPARKKVERARDDPAMLLVTYEGDHRHP 270 69***********************988**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF10533	2.3E-12	165	209	IPR018872	Zn-cluster domain
Gene3D	G3DSA:2.20.25.80	1.0E-32	199	272	IPR003657	WRKY domain
PROSITE profile	PS50811	31.181	206	272	IPR003657	WRKY domain
SuperFamily	SSF118290	8.76E-27	209	272	IPR003657	WRKY domain
SMART	SM00774	1.3E-36	211	271	IPR003657	WRKY domain
Pfam	PF03106	1.0E-26	213	270	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 283 aa Download sequence Send to blast
MAVDLAAFPA FFDDQTAIEE AATAGLQSMN HLIHLLSKQH HHHHHHHSES PNNIDLNSSL 60 LTDFTVSKFK RLISLLNRTG HARFRRGPSD SPNPVLNSLD PPQKTHFSKL NFSPVSKVPE 120 SRDSTTTSSF VSTVTGDGSV SNGKLDLSVY ATPPANAGKP PLAMKSKCHD VSGFGCKVPN 180 SKLCHCAKRR KSGMKKTVKV PAISSKIADI PSDEYSWRKY GQKPIKGSPY PRGYYRCSSV 240 KGCPARKKVE RARDDPAMLL VTYEGDHRHP HPTVTDGVSQ KS*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	2e-20	207	272	10	74	WRKY transcription factor 1
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Regulates rhizobacterium B.cereus AR156-induced systemic resistance (ISR) to P.syringae pv. tomato DC3000, probably by activating the jasmonic acid (JA)- signaling pathway (PubMed:26433201). {ECO:0000250, ECO:0000269\|PubMed:26433201}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Strongly during leaf senescence (PubMed:11722756). Repressed by rhizobacterium B.cereus AR156 in leaves, and to a lower extent, by P.fluorescens WCS417r (PubMed:26433201). {ECO:0000269\|PubMed:11722756, ECO:0000269\|PubMed:26433201}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	GU984010	0.0	GU984010.1 Cucumis sativus WRKY protein (WRKY5) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	NP_001292683.1	0.0	probable WRKY transcription factor 11
Swissprot	Q9SV15	8e-89	WRK11_ARATH; Probable WRKY transcription factor 11
TrEMBL	E7CEV9	0.0	E7CEV9_CUCSA; WRKY protein
STRING	XP_004146340.1	0.0	(Cucumis sativus)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF1314	34	98

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G31550.1	1e-81	WRKY DNA-binding protein 11

Link Out ? help Back to Top
Phytozome	Cucsa.282040.1
Entrez Gene	101204382

Publications ? help Back to Top
Haas BJ, et al. Full-length messenger RNA sequences greatly improve genome annotation. Genome Biol., 2002. 3(6): p. RESEARCH0029 [PMID:12093376] Brand LH,Kirchler T,Hummel S,Chaban C,Wanke D DPI-ELISA: a fast and versatile method to specify the binding of plant transcription factors to DNA in vitro. Plant Methods, 2010. 6: p. 25 [PMID:21108821] Ling J, et al. Genome-wide analysis of WRKY gene family in Cucumis sativus. BMC Genomics, 2011. 12: p. 471 [PMID:21955985] Brand LH, et al. Screening for protein-DNA interactions by automatable DNA-protein interaction ELISA. PLoS ONE, 2013. 8(10): p. e75177 [PMID:24146751] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Jiang CH, et al. Transcription factors WRKY70 and WRKY11 served as regulators in rhizobacterium Bacillus cereus AR156-induced systemic resistance to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis. J. Exp. Bot., 2016. 67(1): p. 157-74 [PMID:26433201] Ali MA, et al. Transcription factors WRKY11 and WRKY17 are involved in abiotic stress responses in Arabidopsis. J. Plant Physiol., 2018. 226: p. 12-21 [PMID:29689430]