PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID GSBRNA2T00042382001
Common NameGSBRNA2T00042382001
Organism
Brassica napus
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica
Family bHLH
Protein Properties Length: 372aa    MW: 42070.3 Da    PI: 8.8625
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
GSBRNA2T00042382001genomeGenoscopeView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH33.57.3e-11175226155
                          CHHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS
                  HLH   1 rrrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                          +r++  ++Er+RR+++N+ +  Lr+l+P +   + k+ + a+i   ++e++++L+
  GSBRNA2T00042382001 175 QRMTRIMVERKRRKQMNEHLRVLRTLMPGS---YVKRGNQASIIGGVIEFVRELE 226
                          69999*************************...9*****************9985 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
CDDcd000832.25E-8173223No hitNo description
Gene3DG3DSA:4.10.280.101.5E-11173227IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profilePS5088813.98174225IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474596.94E-14174242IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000104.0E-8175226IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003532.3E-7180231IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd048730.00957312356No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0046983Molecular Functionprotein dimerization activity
Sequence ? help Back to Top
Protein Sequence    Length: 372 aa     Download sequence    Send to blast
MDKYYSASNF FGKPLGGHDS NSSGMIDYTL NGDLQPQKQP MLQQQPHQKT FIDMMHFADF  60
GPKLALNRTN NQAVQETGFD PVCFLKFPVL KERIEDHIRT QHLMSSHRTP QEGGECGGNI  120
GCVFLEDQDN NSRRLRFIRG GENEDRDNNN VTTKEVNSKR KRARTSKTIE EVESQRMTRI  180
MVERKRRKQM NEHLRVLRTL MPGSYVKRGN QASIIGGVIE FVRELEELLP CLESQKRRRI  240
LEETGHRQTG GMTRPTNPSS CPVTRVANQT QPLIITKNLT ELEGGGGLLE EMTENKSCLA  300
DVEVKLLGFD AMIKILSKRR LGQLLMTMAA LEDLHLSILH TSITTMEQTV LYSFIVKASS  360
TCLHIIILIG V*
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1235239KRRRI
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Not expressed in meristemoids, but strongly expressed in guard mother cells (GMCs) and in young guard cells (at protein level) (PubMed:17088607). Expressed at the transition to terminal stomatal differentiation, just before and after the symmetric division of stomatal differentiation, being confined to late-stage GMC and to young, still differentiating guard cells (PubMed:24571519). {ECO:0000269|PubMed:17088607, ECO:0000269|PubMed:24571519}.
UniprotTISSUE SPECIFICITY: Resctricted to stomatal cell lineages (at protein level). Expressed in roots, leaves, stems, and flowers. {ECO:0000269|PubMed:12679534, ECO:0000269|PubMed:17088607}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription activator (PubMed:17088607, PubMed:17183265, PubMed:17183267). Together with MYB88 and MYB124, ensures that stomata contain just two guard cells (GCs) by enforcing a single symmetric precursor cell division before stomatal maturity (PubMed:24571519). Together with SPCH and MUTE, regulates the stomata formation. Required to promote differentiation and morphogenesis of stomatal guard cells and to halt proliferative divisions in their immediate precursors. Mediates the formation of stomata (PubMed:17088607, PubMed:17183265, PubMed:17183267). Prevents histone H3K27me3 marks and derepresses stem cell gene expression (PubMed:24654956). {ECO:0000269|PubMed:17088607, ECO:0000269|PubMed:17183265, ECO:0000269|PubMed:17183267, ECO:0000269|PubMed:24571519, ECO:0000269|PubMed:24654956}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapGSBRNA2T00042382001
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Inhibited by low relative humidity (LRH) via epigenetic CG methylation, thus leading to a reduced stomatal index. {ECO:0000269|PubMed:22442411}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_013631585.10.0PREDICTED: transcription factor FAMA-like
SwissprotQ56YJ81e-167FAMA_ARATH; Transcription factor FAMA
TrEMBLA0A078JGK70.0A0A078JGK7_BRANA; BnaCnng44210D protein
STRINGBo1g101200.10.0(Brassica oleracea)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM55482649
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT3G24140.11e-158bHLH family protein
Publications ? help Back to Top
  1. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]
  2. Chalhoub B, et al.
    Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome.
    Science, 2014. 345(6199): p. 950-3
    [PMID:25146293]
  3. Matos JL, et al.
    Irreversible fate commitment in the Arabidopsis stomatal lineage requires a FAMA and RETINOBLASTOMA-RELATED module.
    Elife, 2015.
    [PMID:25303364]
  4. Chen L, et al.
    NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis.
    Development, 2016. 143(9): p. 1600-11
    [PMID:26989174]
  5. Lee JH,Jung JH,Park CM
    Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis.
    Plant Cell, 2017. 29(11): p. 2817-2830
    [PMID:29070509]
  6. Han X, et al.
    Jasmonate Negatively Regulates Stomatal Development in Arabidopsis Cotyledons.
    Plant Physiol., 2018. 176(4): p. 2871-2885
    [PMID:29496884]
  7. Li X,Yang R,Chen H
    The Arabidopsis thaliana Mediator subunit MED8 regulates plant immunity to Botrytis Cinerea through interacting with the basic helix-loop-helix (bHLH) transcription factor FAMA.
    PLoS ONE, 2018. 13(3): p. e0193458
    [PMID:29513733]