PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

GSBRNA2T00060200001

Common Name

GSBRNA2T00060200001

Organism

Brassica napus

Taxonomic ID

3708

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica

Family

C2H2

Protein Properties

Length: 448aa MW: 49459.3 Da PI: 5.7379

Description

C2H2 family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
GSBRNA2T00060200001	genome	Genoscope	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	zf-C2H2	14.2	0.00013	123	145	1	23
EEETTTTEEESSHHHHHHHHHHT CS zf-C2H2 1 ykCpdCgksFsrksnLkrHirtH 23 ++Cp+Cg +F++ ++ +H ++ GSBRNA2T00060200001 123 FECPICGNPFKTEEEVSVHVESC 145 89*****************9876 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50157	8.725	123	150	IPR007087	Zinc finger, C2H2
CDD	cd09212	4.74E-22	158	250	No hit	No description
SuperFamily	SSF143503	5.89E-19	159	229	IPR018997	PUB domain
Pfam	PF09409	3.3E-18	163	233	IPR018997	PUB domain
SMART	SM00580	2.6E-23	167	234	IPR018997	PUB domain
SuperFamily	SSF54236	2.53E-12	313	422	IPR029071	Ubiquitin-related domain
Gene3D	G3DSA:3.10.20.90	1.4E-7	338	421	No hit	No description
PROSITE profile	PS50033	10.161	338	422	IPR001012	UBX domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0050832	Biological Process	defense response to fungus
GO:0005515	Molecular Function	protein binding
GO:0046872	Molecular Function	metal ion binding

Sequence ? help Back to Top
Protein Sequence Length: 448 aa Download sequence Send to blast
MDDVKDKLKG FMKKVNPSSS SGKFKGSGRV LGSSSSAPVN PIQNRFNPPQ SSKPNPNQTP 60 RPTKPSPSPS PSAALPEKPV SSGSQKKPDP DPVRAPPQDG FDPFGAFITS SNRSQNGYSL 120 NMFECPICGN PFKTEEEVSV HVESCLGEDT TTNGDEASSE EKDKSESSVD VLLRLLKNIV 180 KEPENGKFRK IRMSNAKIKE AIGDVAGGVE LLELVGFELK EENDEIWAVM DVPGGDQTQL 240 INEVVGMLEK RKIEMTRNDE VLPVEPVAAA PKKIDREIRV FFSVAENVAS RIEVPDSFYS 300 LSADEIKREA DLRRKKIAES QLLIPRSYKE KQAKAARKRY KRSMIRVQFP DGVVLQGVFA 360 PWEPTFALYE FVSSALKEPS LQFELLDPVL VKRRVIPHTP APGQKPRTLE DEELVPSALI 420 KFRPIETASL VYTGLCNELL EISEPLT*

Sequence ? help Back to Top

Protein Sequence Length: 448 aa Download sequence Send to blast

MDDVKDKLKG FMKKVNPSSS SGKFKGSGRV LGSSSSAPVN PIQNRFNPPQ SSKPNPNQTP  60
RPTKPSPSPS PSAALPEKPV SSGSQKKPDP DPVRAPPQDG FDPFGAFITS SNRSQNGYSL  120
NMFECPICGN PFKTEEEVSV HVESCLGEDT TTNGDEASSE EKDKSESSVD VLLRLLKNIV  180
KEPENGKFRK IRMSNAKIKE AIGDVAGGVE LLELVGFELK EENDEIWAVM DVPGGDQTQL  240
INEVVGMLEK RKIEMTRNDE VLPVEPVAAA PKKIDREIRV FFSVAENVAS RIEVPDSFYS  300
LSADEIKREA DLRRKKIAES QLLIPRSYKE KQAKAARKRY KRSMIRVQFP DGVVLQGVFA  360
PWEPTFALYE FVSSALKEPS LQFELLDPVL VKRRVIPHTP APGQKPRTLE DEELVPSALI  420
KFRPIETASL VYTGLCNELL EISEPLT*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
5ifw_A	2e-12	293	422	23	146	Tether containing UBX domain for GLUT4
Search in ModeBase

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	335	343	ARKRYKRSM

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Bna.9702	0.0	leaf\| seed

Functional Description ? help Back to Top
Source	Description
UniProt	Facilitates the interaction of SYP31 and CDC48A, thereby regulating an CDC48A membrane-associated function (Ref.8). Appears to act as a negative regulator mediating the powdery mildew-plant interaction (PubMed:19176722, PubMed:23301616). {ECO:0000269\|PubMed:19176722, ECO:0000269\|PubMed:23301616, ECO:0000269\|Ref.8}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	GSBRNA2T00060200001

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced in response to powdery mildew and by benzothiadiazole (BTH) treatment. {ECO:0000269\|PubMed:19176722}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK118911	1e-175	AK118911.1 Arabidopsis thaliana At2g01650 mRNA for unknown protein, complete cds, clone: RAFL21-25-I03.
GenBank	AY572782	1e-175	AY572782.1 Arabidopsis thaliana CDC48-interacting UBX-domain protein (PUX2) mRNA, complete cds.
GenBank	BT005408	1e-175	BT005408.1 Arabidopsis thaliana clone U51117 unknown protein (At2g01650) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_013707193.1	0.0	plant UBX domain-containing protein 2-like
Swissprot	Q9ZU93	0.0	PUX2_ARATH; Plant UBX domain-containing protein 2
TrEMBL	A0A078HCW6	0.0	A0A078HCW6_BRANA; BnaC07g20990D protein
STRING	Bo7g078630.1	0.0	(Brassica oleracea)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM9835	16	18

Publications ? help Back to Top
Suzuki T,Park H,Till EA,Lennarz WJ The PUB domain: a putative protein-protein interaction domain implicated in the ubiquitin-proteasome pathway. Biochem. Biophys. Res. Commun., 2001. 287(5): p. 1083-7 [PMID:11587532] Rancour DM,Park S,Knight SD,Bednarek SY Plant UBX domain-containing protein 1, PUX1, regulates the oligomeric structure and activity of arabidopsis CDC48. J. Biol. Chem., 2004. 279(52): p. 54264-74 [PMID:15498773] Chandran D,Rickert J,Cherk C,Dotson BR,Wildermuth MC Host cell ploidy underlying the fungal feeding site is a determinant of powdery mildew growth and reproduction. Mol. Plant Microbe Interact., 2013. 26(5): p. 537-45 [PMID:23301616] Chalhoub B, et al. Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome. Science, 2014. 345(6199): p. 950-3 [PMID:25146293]