PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

GSBRNA2T00072914001

Common Name

GSBRNA2T00072914001

Organism

Brassica napus

Taxonomic ID

3708

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica

Family

NAC

Protein Properties

Length: 538aa MW: 60706.8 Da PI: 4.2378

Description

NAC family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
GSBRNA2T00072914001	genome	Genoscope	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	NAM	164.2	4.6e-51	13	140	1	128
NAM 1 lppGfrFhPtdeelvveyLkkkvegkkleleevikevdiykvePwdLp.k.kvkaeekewyfFskrdkkyatgkrknratksgyWkatgk 88 l+pGfrFhPtdeelv +yLk+k+ +k++++ ++i+ +++yk+ePwdLp + k+k+++ ewyfFs d+ky++g+++nrat+ gyWk+tgk GSBRNA2T00072914001 13 LAPGFRFHPTDEELVRYYLKRKICNKPFKF-DAISVTHVYKSEPWDLPsQsKLKSRDLEWYFFSVLDNKYSNGSKTNRATEMGYWKTTGK 101 579************************9.99**********95247777888**************************** PP NAM 89 dkevlskkgelvglkktLvfykgrapkgektdWvmheyrl 128 d+e+ + ++++vg+kktLv++kgrap+ge+++Wvmheyrl GSBRNA2T00072914001 102 DREIRN-GSRVVGMKKTLVYHKGRAPRGERSNWVMHEYRL 140 *9.9****************************98 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF101941	1.2E-59	10	163	IPR003441	NAC domain
PROSITE profile	PS51005	56.47	13	163	IPR003441	NAC domain
Pfam	PF02365	3.9E-27	15	140	IPR003441	NAC domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009819	Biological Process	drought recovery
GO:0010150	Biological Process	leaf senescence
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:2000377	Biological Process	regulation of reactive oxygen species metabolic process
GO:0005634	Cellular Component	nucleus
GO:0005737	Cellular Component	cytoplasm
GO:0005886	Cellular Component	plasma membrane
GO:0016021	Cellular Component	integral component of membrane
GO:0003677	Molecular Function	DNA binding
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 538 aa Download sequence Send to blast
MGRRGGSSET SSLAPGFRFH PTDEELVRYY LKRKICNKPF KFDAISVTHV YKSEPWDLPS 60 QSKLKSRDLE WYFFSVLDNK YSNGSKTNRA TEMGYWKTTG KDREIRNGSR VVGMKKTLVY 120 HKGRAPRGER SNWVMHEYRL VDDDIVKAGV QKDAYVLCKI FQKSGSGPKN GEQYGAPFVE 180 EEWEEEDCMT FEPNQDPGSL EDQAYVDIHD IDQKIDVYDA IPIHLGFDQG ESSNNVETNY 240 SDTTNYIQPG NYVHDNFEGP VDLSEEEQKL IIRDALFPDE ENGCGVQEES TANLQSSDNI 300 FETDASGYNA FPVEGNYFTG EEFLNPNDGL YLETNDLNST DQDGFNFEDY LTFFDEDDQN 360 LTFDPSQLMG TEDVVPDQEE LFQMAETKEL EKEEASGGKQ VVEEKENDEA CCSKQVNADT 420 TEFEPDYKNS VLKKASHMFG AIPTPTEFAS EILTKDGVVR LQAGQSSGSV HVSMITVSDS 480 NMGWSYSKNG DLSFGMVQEN VPGKSENNLT RVMLIFICFW VLVLSVSFKV STLVSSR*

Sequence ? help Back to Top

Protein Sequence Length: 538 aa Download sequence Send to blast

MGRRGGSSET SSLAPGFRFH PTDEELVRYY LKRKICNKPF KFDAISVTHV YKSEPWDLPS  60
QSKLKSRDLE WYFFSVLDNK YSNGSKTNRA TEMGYWKTTG KDREIRNGSR VVGMKKTLVY  120
HKGRAPRGER SNWVMHEYRL VDDDIVKAGV QKDAYVLCKI FQKSGSGPKN GEQYGAPFVE  180
EEWEEEDCMT FEPNQDPGSL EDQAYVDIHD IDQKIDVYDA IPIHLGFDQG ESSNNVETNY  240
SDTTNYIQPG NYVHDNFEGP VDLSEEEQKL IIRDALFPDE ENGCGVQEES TANLQSSDNI  300
FETDASGYNA FPVEGNYFTG EEFLNPNDGL YLETNDLNST DQDGFNFEDY LTFFDEDDQN  360
LTFDPSQLMG TEDVVPDQEE LFQMAETKEL EKEEASGGKQ VVEEKENDEA CCSKQVNADT  420
TEFEPDYKNS VLKKASHMFG AIPTPTEFAS EILTKDGVVR LQAGQSSGSV HVSMITVSDS  480
NMGWSYSKNG DLSFGMVQEN VPGKSENNLT RVMLIFICFW VLVLSVSFKV STLVSSR*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1ut4_A	3e-43	12	169	16	171	NO APICAL MERISTEM PROTEIN
1ut4_B	3e-43	12	169	16	171	NO APICAL MERISTEM PROTEIN
1ut7_A	3e-43	12	169	16	171	NO APICAL MERISTEM PROTEIN
1ut7_B	3e-43	12	169	16	171	NO APICAL MERISTEM PROTEIN
3swm_A	3e-43	12	169	19	174	NAC domain-containing protein 19
3swm_B	3e-43	12	169	19	174	NAC domain-containing protein 19
3swm_C	3e-43	12	169	19	174	NAC domain-containing protein 19
3swm_D	3e-43	12	169	19	174	NAC domain-containing protein 19
3swp_A	3e-43	12	169	19	174	NAC domain-containing protein 19
3swp_B	3e-43	12	169	19	174	NAC domain-containing protein 19
3swp_C	3e-43	12	169	19	174	NAC domain-containing protein 19
3swp_D	3e-43	12	169	19	174	NAC domain-containing protein 19
4dul_A	3e-43	12	169	16	171	NAC domain-containing protein 19
4dul_B	3e-43	12	169	16	171	NAC domain-containing protein 19
Search in ModeBase

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Bna.11615	0.0	seed

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: Expressed in the anthers and the upper parts of the stamen filaments after stage 7 to 9 of flower budding. In stage 10 flower buds, expressed in the anthers and pollen grains. Weakly expressed in mature flowers at stage 12. {ECO:0000269\|PubMed:24323506}.
Uniprot	TISSUE SPECIFICITY: Expressed in roots, rosette leaves, cauline leaves, shoot apex and stems. {ECO:0000269\|PubMed:17158162}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcriptional activator activated by proteolytic cleavage through regulated intramembrane proteolysis (RIP) (Ref.6, PubMed:24323506, PubMed:25219309). Promotes reactive oxygen species (ROS) production during drought-induced leaf senescence. In response to abscisic acid (ABA)-mediated drought stress signals, binds directly to the promoters of RBOHC and RBOHE genes, encoding ROS biosynthetic enzymes, resulting in ROS accumulation and triggering leaf senescence via programmed cell death (PCD). ROS-induced leaf senescence sustains plant survival under drought conditions (PubMed:22313226). Involved in heat stress response. Modulates PCD through a ROS-mediated positive feedback control under heat stress conditions. This may provide an adaptation strategy for plant survival under extreme heat stress conditions (PubMed:25219309). Acts as repressor in preventing anther dehiscence during stamen development by suppressing genes that participate in jasmonic acid (JA) biosynthesis, such as DAD1, AOS, AOC3, OPR3 and 4CLL5/OPCL1 (PubMed:24323506). {ECO:0000269\|PubMed:22313226, ECO:0000269\|PubMed:24323506, ECO:0000269\|PubMed:25219309, ECO:0000269\|Ref.6}.

Binding Motif ? help Back to Top
Motif ID	Method	Source	Motif file
MP00343	DAP	Transfer from AT3G10500	Download

Cis-element ? help Back to Top
Source	Link
PlantRegMap	GSBRNA2T00072914001

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By abscisic acid (ABA) (PubMed:22313226, PubMed:25219309). Induced by heat shock (PubMed:25219309). Induced by cold, drought stress and methyl methanesulfonate (MMS) treatment (PubMed:17158162). {ECO:0000269\|PubMed:17158162, ECO:0000269\|PubMed:22313226, ECO:0000269\|PubMed:25219309}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	Retrieve

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_022545085.1	0.0	NAC domain-containing protein 53-like
Swissprot	Q949N0	0.0	NAC53_ARATH; NAC domain-containing protein 53
TrEMBL	A0A078HVH3	0.0	A0A078HVH3_BRANA; BnaA01g31650D protein
STRING	Bo1g143830.1	0.0	(Brassica oleracea)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM3192	27	62

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G10500.1	0.0	NAC domain containing protein 53

Publications ? help Back to Top
Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Chalhoub B, et al. Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome. Science, 2014. 345(6199): p. 950-3 [PMID:25146293] Vermeirssen V,De Clercq I,Van Parys T,Van Breusegem F,Van de Peer Y Arabidopsis ensemble reverse-engineered gene regulatory network discloses interconnected transcription factors in oxidative stress. Plant Cell, 2014. 26(12): p. 4656-79 [PMID:25549671] Gladman NP,Marshall RS,Lee KH,Vierstra RD The Proteasome Stress Regulon Is Controlled by a Pair of NAC Transcription Factors in Arabidopsis. Plant Cell, 2016. 28(6): p. 1279-96 [PMID:27194708] Sanz-Fernández M, et al. Screening Arabidopsis mutants in genes useful for phytoremediation. J. Hazard. Mater., 2017. 335: p. 143-151 [PMID:28441590]