PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

GSBRNA2T00124914001

Common Name

GSBRNA2T00124914001

Organism

Brassica napus

Taxonomic ID

3708

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica

Family

Dof

Protein Properties

Length: 215aa MW: 22929 Da PI: 9.8174

Description

Dof family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
GSBRNA2T00124914001	genome	Genoscope	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	zf-Dof	123.4	7.3e-39	71	130	3	62
zf-Dof 3 ekalkcprCdstntkfCyynnyslsqPryfCkaCrryWtkGGalrnvPvGggrrknkkss 62 e+alkcprC+stntkfCy+nnysl+qPr+fCk+CrryWt+GG+lrnvPvGgg r+n++++ GSBRNA2T00124914001 71 EAALKCPRCESTNTKFCYFNNYSLTQPRHFCKSCRRYWTRGGSLRNVPVGGGYRRNRHTK 130 7789*****************************************************987 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
ProDom	PD007478	2.0E-34	58	128	IPR003851	Zinc finger, Dof-type
Pfam	PF02701	2.1E-32	73	128	IPR003851	Zinc finger, Dof-type
PROSITE profile	PS50884	28.966	74	128	IPR003851	Zinc finger, Dof-type
PROSITE pattern	PS01361	0	76	112	IPR003851	Zinc finger, Dof-type

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003677	Molecular Function	DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 215 aa Download sequence Send to blast
MVFSSIQAYL DSSNWQQAPP SNHNQGGAGA SDTGGHGVFP QPQSNSSGGS GSIRPGSMVD 60 RARQANVAMP EAALKCPRCE STNTKFCYFN NYSLTQPRHF CKSCRRYWTR GGSLRNVPVG 120 GGYRRNRHTK SSSNNNSTAT SNNTSFTSAA SGNASTISAI LSSNYGGNHE SILSQILYPG 180 RFSSVKGEDN PYKWVNVNGN CSSWIDLSTF GSSS*

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: Specific to the midveins, containing narrow procambial cell files. Expressed in procambial cells of leaf primordia, roots and embryos, prior to the completion of xylem differentiation. {ECO:0000269\|PubMed:17583520}.
Uniprot	TISSUE SPECIFICITY: Specific to the vascular tissues (PubMed:17583520). The PEAR proteins (e.g. DOF2.4, DOF5.1, DOF3.2, DOF1.1, DOF5.6 and DOF5.3) form a short-range concentration gradient that peaks at protophloem sieve elements (PSE) (PubMed:30626969). {ECO:0000269\|PubMed:17583520, ECO:0000269\|PubMed:30626969}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence (By similarity). Probably involved in early processes for vascular development (PubMed:17583520). The PEAR proteins (e.g. DOF2.4, DOF5.1, DOF3.2, DOF1.1, DOF5.6 and DOF5.3) activate gene expression that promotes radial growth of protophloem sieve elements. Triggers the transcription of HD-ZIP III genes, especially in the central domain of vascular tissue (PubMed:30626969). {ECO:0000250\|UniProtKB:Q9M2U1, ECO:0000269\|PubMed:17583520, ECO:0000269\|PubMed:30626969}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	GSBRNA2T00124914001

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By cytokinin in procambium. Antagonized by the HD-ZIP III proteins and by mobile miR165 and miR166 microRNAs. {ECO:0000269\|PubMed:30626969}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AC189307	1e-127	AC189307.2 Brassica rapa subsp. pekinensis cultivar Inbred line 'Chiifu' clone KBrB033O04, complete sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_013627183.1	1e-103	PREDICTED: dof zinc finger protein DOF2.4-like
Swissprot	O80928	4e-80	DOF24_ARATH; Dof zinc finger protein DOF2.4
TrEMBL	A0A078F326	1e-155	A0A078F326_BRANA; BnaA03g17300D protein
STRING	Bo3g031830.1	1e-102	(Brassica oleracea)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM11354	17	32

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G37590.1	2e-64	DNA binding with one finger 2.4

Publications ? help Back to Top
Heyndrickx KS,Vandepoele K Systematic identification of functional plant modules through the integration of complementary data sources. Plant Physiol., 2012. 159(3): p. 884-901 [PMID:22589469] Chalhoub B, et al. Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome. Science, 2014. 345(6199): p. 950-3 [PMID:25146293] Miyashima S, et al. Mobile PEAR transcription factors integrate positional cues to prime cambial growth. Nature, 2019. 565(7740): p. 490-494 [PMID:30626969]