PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

GSBRNA2T00144969001

Common Name

GSBRNA2T00144969001

Organism

Brassica napus

Taxonomic ID

3708

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica

Family

bHLH

Protein Properties

Length: 569aa MW: 64569 Da PI: 4.8268

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
GSBRNA2T00144969001	genome	Genoscope	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	39.1	1.3e-12	314	357	7	55
HHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 7 erErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +ErrRR+++N+++ Lr+l+P +Kl+ a+iL A++Y+k+Lq GSBRNA2T00144969001 314 LAERRRRKKLNDRLYALRSLVPRI-----TKLDRASILGDAINYVKELQ 357 68*******************7.....7****************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF14215	1.6E-15	10	149	IPR025610	Transcription factor MYC/MYB N-terminal
CDD	cd00083	4.18E-14	306	359	No hit	No description
PROSITE profile	PS50888	16.488	307	356	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	7.07E-17	310	371	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	3.8E-15	313	362	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	3.3E-10	314	357	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	7.1E-16	314	370	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 569 aa Download sequence Send to blast
MESTMQNLLE KLRPLVGARA WDYCVLWRLN EDQRFVKWIG CCCGGTQLIE ENGTEEFSIE 60 GCRDVMFHHP RTKSCEFLDH LPSSIPLDSG GIYAETLLTN QTGWLSESSE PGFMQETICT 120 RVLIPIPGGL VELFATRHVA EDQNVVDFVM GNCNMLMDET VTINMMVADE VESKPYGMLC 180 GDIHQKGSKD EEMMNLPLPY DISTDQMRVN FLPQMSEYEA QQHLKVKSDY HHQDLGYLPE 240 NGSKEMMGMN PFSTEDGMSV MGEPSLLVNE QQIANDKEMN ENGTGSDCSD QIDDEDDQKY 300 KKKTGKHSQA KNLLAERRRR KKLNDRLYAL RSLVPRITKL DRASILGDAI NYVKELQIEA 360 KELQDELEEN SETEDGANRQ RGGVSLNGTV VTGFHPGISC NSNVPNLKQD VDIENANDKG 420 QEMEPQVDVA QLDGIEFFVK VICEYKPGGF TRLMEALDSL GLEVTNANTT RFLSLVSNVF 480 KVEKTDSEMV PAEHVRNSLL EITRNTSRGW HDDQMATGSI QNEKNEVDYQ HYDDHHHHSG 540 HHHLNDHQMN HSAQHHHHHQ HINHYQNQ*

Sequence ? help Back to Top

Protein Sequence Length: 569 aa Download sequence Send to blast

MESTMQNLLE KLRPLVGARA WDYCVLWRLN EDQRFVKWIG CCCGGTQLIE ENGTEEFSIE  60
GCRDVMFHHP RTKSCEFLDH LPSSIPLDSG GIYAETLLTN QTGWLSESSE PGFMQETICT  120
RVLIPIPGGL VELFATRHVA EDQNVVDFVM GNCNMLMDET VTINMMVADE VESKPYGMLC  180
GDIHQKGSKD EEMMNLPLPY DISTDQMRVN FLPQMSEYEA QQHLKVKSDY HHQDLGYLPE  240
NGSKEMMGMN PFSTEDGMSV MGEPSLLVNE QQIANDKEMN ENGTGSDCSD QIDDEDDQKY  300
KKKTGKHSQA KNLLAERRRR KKLNDRLYAL RSLVPRITKL DRASILGDAI NYVKELQIEA  360
KELQDELEEN SETEDGANRQ RGGVSLNGTV VTGFHPGISC NSNVPNLKQD VDIENANDKG  420
QEMEPQVDVA QLDGIEFFVK VICEYKPGGF TRLMEALDSL GLEVTNANTT RFLSLVSNVF  480
KVEKTDSEMV PAEHVRNSLL EITRNTSRGW HDDQMATGSI QNEKNEVDYQ HYDDHHHHSG  540
HHHLNDHQMN HSAQHHHHHQ HINHYQNQ*

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	300	321	KKKTGKHSQAKNLLAERRRRKK
2	315	322	ERRRRKKL

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Bna.2263	0.0	flower

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: First observed a bud stage of 0.6-mm, shortly after microspore release from the postmeiotic tetrads. Later restricted within the tapetum, microspores and anther locule. Still visible within the haploid nuclei, each of which had migrated to the pollen cell wall prior to pollen mitosis I. Not expressed later. {ECO:0000269\|PubMed:12535353}.
Uniprot	TISSUE SPECIFICITY: Mostly expressed in closed, post-meiotic buds, and, to a lower extent, in pre-meiotic buds. Detected in leaves, stems, and flowers. {ECO:0000269\|PubMed:12535353, ECO:0000269\|PubMed:12679534}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Plays a crucial role in tapetum development. Required for male fertility and pollen differentiation, especially during the post-meiotic transcriptional regulation of microspore development within the developing anther (PubMed:12535353). Binds E-box regions in the AHL16/TEK promoter. {ECO:0000269\|PubMed:12535353, ECO:0000269\|PubMed:24804694}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	GSBRNA2T00144969001

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AJ511991	0.0	AJ511991.1 Brassica napus mRNA for bHLH transcription factor.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_013724907.2	0.0	transcription factor ABORTED MICROSPORES isoform X1
Refseq	XP_022562175.1	0.0	transcription factor ABORTED MICROSPORES isoform X1
Swissprot	Q9ZVX2	0.0	AMS_ARATH; Transcription factor ABORTED MICROSPORES
TrEMBL	A0A3P6EG25	0.0	A0A3P6EG25_BRAOL; Uncharacterized protein
STRING	Bo7g016210.1	0.0	(Brassica oleracea)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM7589	27	41

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G16910.1	0.0	bHLH family protein

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Jiang J,Zhang Z,Cao J Pollen wall development: the associated enzymes and metabolic pathways. Plant Biol (Stuttg), 2013. 15(2): p. 249-63 [PMID:23252839] Xu J, et al. ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis. Plant Cell, 2014. 26(4): p. 1544-1556 [PMID:24781116] Chalhoub B, et al. Plant genetics. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome. Science, 2014. 345(6199): p. 950-3 [PMID:25146293] Qian H, et al. Trace concentrations of imazethapyr (IM) affect floral organs development and reproduction in Arabidopsis thaliana: IM-induced inhibition of key genes regulating anther and pollen biosynthesis. Ecotoxicology, 2015. 24(1): p. 163-71 [PMID:25348600] Xiong SX, et al. The transcription factors MS188 and AMS form a complex to activate the expression of CYP703A2 for sporopollenin biosynthesis in Arabidopsis thaliana. Plant J., 2016. 88(6): p. 936-946 [PMID:27460657] Ferguson AC, et al. Biphasic regulation of the transcription factor ABORTED MICROSPORES (AMS) is essential for tapetum and pollen development in Arabidopsis. New Phytol., 2017. 213(2): p. 778-790 [PMID:27787905] Lou Y, et al. Positive regulation of AMS by TDF1 and the formation of a TDF1-AMS complex are required for anther development in Arabidopsis thaliana. New Phytol., 2018. 217(1): p. 378-391 [PMID:28940573] Bi H,Fan W,Zhang P C4 Protein of Sweet Potato Leaf Curl Virus Regulates Brassinosteroid Signaling Pathway through Interaction with AtBIN2 and Affects Male Fertility in Arabidopsis. Front Plant Sci, 2017. 8: p. 1689 [PMID:29021807]