PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Glyma.02G129100.1.p

Common Name

GLYMA_02G129100, LOC100793349

Organism

Glycine max

Taxonomic ID

3847

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Phaseoleae; Glycine; Soja

Family

bHLH

Protein Properties

Length: 447aa MW: 50758.1 Da PI: 5.3265

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Glyma.02G129100.1.p	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	37.9	3.2e-12	239	290	1	55
CHHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 1 rrrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +r++h ++Er+RR+++N+ + Lr+l+P + + ++ + a+i A+e++++L+ Glyma.02G129100.1.p 239 QRMTHIAVERNRRKQMNEHLRVLRSLMPGS---YVQRGDQASIIGGAIEFVRELE 290 79**************************...9***************9995 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:4.10.280.10	1.6E-13	237	293	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	2.25E-12	237	294	No hit	No description
SuperFamily	SSF47459	2.49E-16	238	304	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS50888	15.428	238	289	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	1.5E-9	239	290	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	3.4E-9	244	295	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd04873	0.00476	372	436	No hit	No description

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 447 aa Download sequence Send to blast
MEKDHNYSTP PPPPLSMPPS FNTLDYSLDQ QQHHHLYAPN QHQQHLMMKF QQGSGDENNN 60 IGSMVDYMPQ TTTTLPPHGF YGTATSAATT SYDKLSFADV MQFADFGPKL ALNQAKSCEE 120 SAIDPVYFLK FPVLNDKMEE DHQQNMMVNN DDPDGDEAEN HHHLDERFNL VSVEGKDQGM 180 MMREDEETTR VSDDNNNSVQ IRFLGHEEPQ QKNCAVQENK NGKKKRPRTV KTSEEVESQR 240 MTHIAVERNR RKQMNEHLRV LRSLMPGSYV QRGDQASIIG GAIEFVRELE QLLQCLESQK 300 RRRLLGEAQA RQVGDPSLVA QQQQQPPFFP TLPIPNEQMK LVEMETGLRE ETAECKSCLA 360 DVEVKLLGFD AMIKILSRRR PGQLIKTIAA LEDLQLIILH TNITTIEQTV LYSFNVKVAS 420 DSRFTAEDIA SSVQQIFNFI HANTSM*

Sequence ? help Back to Top

Protein Sequence Length: 447 aa Download sequence Send to blast

MEKDHNYSTP PPPPLSMPPS FNTLDYSLDQ QQHHHLYAPN QHQQHLMMKF QQGSGDENNN  60
IGSMVDYMPQ TTTTLPPHGF YGTATSAATT SYDKLSFADV MQFADFGPKL ALNQAKSCEE  120
SAIDPVYFLK FPVLNDKMEE DHQQNMMVNN DDPDGDEAEN HHHLDERFNL VSVEGKDQGM  180
MMREDEETTR VSDDNNNSVQ IRFLGHEEPQ QKNCAVQENK NGKKKRPRTV KTSEEVESQR  240
MTHIAVERNR RKQMNEHLRV LRSLMPGSYV QRGDQASIIG GAIEFVRELE QLLQCLESQK  300
RRRLLGEAQA RQVGDPSLVA QQQQQPPFFP TLPIPNEQMK LVEMETGLRE ETAECKSCLA  360
DVEVKLLGFD AMIKILSRRR PGQLIKTIAA LEDLQLIILH TNITTIEQTV LYSFNVKVAS  420
DSRFTAEDIA SSVQQIFNFI HANTSM*

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	239	250	RMTHIAVERNRR
2	299	303	KRRRL

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: Not expressed in meristemoids, but strongly expressed in guard mother cells (GMCs) and in young guard cells (at protein level) (PubMed:17088607). Expressed at the transition to terminal stomatal differentiation, just before and after the symmetric division of stomatal differentiation, being confined to late-stage GMC and to young, still differentiating guard cells (PubMed:24571519). {ECO:0000269\|PubMed:17088607, ECO:0000269\|PubMed:24571519}.
Uniprot	TISSUE SPECIFICITY: Resctricted to stomatal cell lineages (at protein level). Expressed in roots, leaves, stems, and flowers. {ECO:0000269\|PubMed:12679534, ECO:0000269\|PubMed:17088607}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator (PubMed:17088607, PubMed:17183265, PubMed:17183267). Together with MYB88 and MYB124, ensures that stomata contain just two guard cells (GCs) by enforcing a single symmetric precursor cell division before stomatal maturity (PubMed:24571519). Together with SPCH and MUTE, regulates the stomata formation. Required to promote differentiation and morphogenesis of stomatal guard cells and to halt proliferative divisions in their immediate precursors. Mediates the formation of stomata (PubMed:17088607, PubMed:17183265, PubMed:17183267). Prevents histone H3K27me3 marks and derepresses stem cell gene expression (PubMed:24654956). {ECO:0000269\|PubMed:17088607, ECO:0000269\|PubMed:17183265, ECO:0000269\|PubMed:17183267, ECO:0000269\|PubMed:24571519, ECO:0000269\|PubMed:24654956}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Glyma.02G129100.1.p

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Inhibited by low relative humidity (LRH) via epigenetic CG methylation, thus leading to a reduced stomatal index. {ECO:0000269\|PubMed:22442411}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AP015043	1e-171	AP015043.1 Vigna angularis var. angularis DNA, chromosome 10, almost complete sequence, cultivar: Shumari.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_006574999.1	0.0	transcription factor FAMA isoform X1
Refseq	XP_028204555.1	0.0	transcription factor FAMA-like isoform X1
Swissprot	Q56YJ8	1e-145	FAMA_ARATH; Transcription factor FAMA
TrEMBL	A0A445LP72	0.0	A0A445LP72_GLYSO; Transcription factor FAMA isoform B
TrEMBL	K7K812	0.0	K7K812_SOYBN; Uncharacterized protein
STRING	GLYMA02G14290.2	0.0	(Glycine max)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF10489	33	40
Representative plant	OGRP253	15	131

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G24140.1	1e-133	bHLH family protein

Link Out ? help Back to Top
Phytozome	Glyma.02G129100.1.p
Entrez Gene	100793349

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Matos JL, et al. Irreversible fate commitment in the Arabidopsis stomatal lineage requires a FAMA and RETINOBLASTOMA-RELATED module. Elife, 2015. [PMID:25303364] Chen L, et al. NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis. Development, 2016. 143(9): p. 1600-11 [PMID:26989174] Lee JH,Jung JH,Park CM Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis. Plant Cell, 2017. 29(11): p. 2817-2830 [PMID:29070509] Han X, et al. Jasmonate Negatively Regulates Stomatal Development in Arabidopsis Cotyledons. Plant Physiol., 2018. 176(4): p. 2871-2885 [PMID:29496884] Li X,Yang R,Chen H The Arabidopsis thaliana Mediator subunit MED8 regulates plant immunity to Botrytis Cinerea through interacting with the basic helix-loop-helix (bHLH) transcription factor FAMA. PLoS ONE, 2018. 13(3): p. e0193458 [PMID:29513733]