PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Lj0g3v0151469.2

Organism

Lotus japonicus

Taxonomic ID

34305

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Loteae; Lotus

Family

AP2

Protein Properties

Length: 188aa MW: 21427.7 Da PI: 5.9473

Description

AP2 family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Lj0g3v0151469.2	genome	Kazusa	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	AP2	52.7	1e-16	83	134	1	55
AP2 1 sgykGVrwdkkrgrWvAeIrd.psengkrkrfslgkfgtaeeAakaaiaarkkleg 55 s+y+GV +++ +grW+A+I + g +k+ +lg++ t eeAa a++ a+ +++g Lj0g3v0151469.2 83 SKYRGVARHHHNGRWEARIGRvF---G-NKYLYLGTYATQEEAATAYDMAAIEYRG 134 89**99******99966...3.6***********************98 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS51032	12.774	1	48	IPR001471	AP2/ERF domain
SMART	SM00380	7.5E-5	2	54	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	1.2E-9	14	50	IPR001471	AP2/ERF domain
SuperFamily	SSF54171	1.44E-9	14	50	IPR016177	DNA-binding domain
SuperFamily	SSF54171	3.2E-18	83	144	IPR016177	DNA-binding domain
CDD	cd00018	3.81E-24	83	144	No hit	No description
Pfam	PF00847	1.9E-12	83	134	IPR001471	AP2/ERF domain
SMART	SM00380	1.2E-24	84	148	IPR001471	AP2/ERF domain
Gene3D	G3DSA:3.30.730.10	3.6E-18	84	143	IPR001471	AP2/ERF domain
PROSITE profile	PS51032	18.334	84	142	IPR001471	AP2/ERF domain
PRINTS	PR00367	8.6E-5	85	96	IPR001471	AP2/ERF domain
PRINTS	PR00367	8.6E-5	124	144	IPR001471	AP2/ERF domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0009414	Biological Process	response to water deprivation
GO:0009651	Biological Process	response to salt stress
GO:0009737	Biological Process	response to abscisic acid
GO:0010187	Biological Process	negative regulation of seed germination
GO:0040008	Biological Process	regulation of growth
GO:0045723	Biological Process	positive regulation of fatty acid biosynthetic process
GO:1901959	Biological Process	positive regulation of cutin biosynthetic process
GO:0000981	Molecular Function	RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003677	Molecular Function	DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 188 aa Download sequence Send to blast
MPHRVFYGDG KQLAAVYLGA YDDEDAAARA YDLAALKYWG QDTILNFPLS NYQDELKEME 60 NQSKEEYIGS LRRKSSGFSR GVSKYRGVAR HHHNGRWEAR IGRVFGNKYL YLGTYATQEE 120 AATAYDMAAI EYRGLNAVTN FDLSRYIKWL HPKTQDQDND EIATKNPEPN DIPNSELELG 180 LVSHQIGP

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Lja.38	1e-120	floral bud\| root

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Mostly expressed in siliques, especially in seeds. Also detected in roots and flowers, and, to a lower extent, in leaves stems and seedlings. {ECO:0000269\|PubMed:15500472, ECO:0000269\|PubMed:15753106}.

Functional Description ? help Back to Top
Source	Description
UniProt	May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). Transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds. Binds to the GCC-box pathogenesis-related promoter element. Promotes sugar uptake and seed oil accumulation by glycolysis. Required for embryo development, seed germination and, indirectly, for seedling establishment. Negative regulator of the ABA-mediated germination inhibition. {ECO:0000250, ECO:0000269\|PubMed:12084821, ECO:0000269\|PubMed:15500472, ECO:0000269\|PubMed:15753106, ECO:0000269\|PubMed:16553903, ECO:0000269\|PubMed:16632590, ECO:0000269\|PubMed:9733529}.
UniProt	Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). {ECO:0000250}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Lj0g3v0151469.2

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Transiantly in leaves by sucrose, but not by abscisic acid (ABA). {ECO:0000269\|PubMed:15753106}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_019458993.1	2e-96	PREDICTED: AP2-like ethylene-responsive transcription factor At1g16060
Swissprot	Q6X5Y6	5e-67	WRI1_ARATH; Ethylene-responsive transcription factor WRI1
Swissprot	Q94AN4	6e-68	AP2L1_ARATH; AP2-like ethylene-responsive transcription factor At1g16060
TrEMBL	A0A1J7HQK8	1e-92	A0A1J7HQK8_LUPAN; Uncharacterized protein
STRING	Gorai.009G008800.1	2e-90	(Gossypium raimondii)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G16060.2	3e-60	ARIA-interacting double AP2 domain protein

Publications ? help Back to Top
Todd J,Post-Beittenmiller D,Jaworski JG KCS1 encodes a fatty acid elongase 3-ketoacyl-CoA synthase affecting wax biosynthesis in Arabidopsis thaliana. Plant J., 1999. 17(2): p. 119-30 [PMID:10074711] Duarte JM, et al. Expression pattern shifts following duplication indicative of subfunctionalization and neofunctionalization in regulatory genes of Arabidopsis. Mol. Biol. Evol., 2006. 23(2): p. 469-78 [PMID:16280546] Kim HU, et al. Ectopic overexpression of castor bean LEAFY COTYLEDON2 (LEC2) in Arabidopsis triggers the expression of genes that encode regulators of seed maturation and oil body proteins in vegetative tissues. FEBS Open Bio, 2013. 4: p. 25-32 [PMID:24363987] Wu XL,Liu ZH,Hu ZH,Huang RZ BnWRI1 coordinates fatty acid biosynthesis and photosynthesis pathways during oil accumulation in rapeseed. J Integr Plant Biol, 2014. 56(6): p. 582-93 [PMID:24393360] Shigematsu H, et al. Structural characterization of the mechanosensitive channel candidate MCA2 from Arabidopsis thaliana. PLoS ONE, 2014. 9(1): p. e87724 [PMID:24475319] Kim HU, et al. Senescence-inducible LEC2 enhances triacylglycerol accumulation in leaves without negatively affecting plant growth. Plant Biotechnol. J., 2015. 13(9): p. 1346-59 [PMID:25790072] Grimberg Å,Carlsson AS,Marttila S,Bhalerao R,Hofvander P Transcriptional transitions in Nicotiana benthamiana leaves upon induction of oil synthesis by WRINKLED1 homologs from diverse species and tissues. BMC Plant Biol., 2015. 15: p. 192 [PMID:26253704] Kanai M,Mano S,Kondo M,Hayashi M,Nishimura M Extension of oil biosynthesis during the mid-phase of seed development enhances oil content in Arabidopsis seeds. Plant Biotechnol. J., 2016. 14(5): p. 1241-50 [PMID:26503031] Li Q, et al. Wrinkled1 Accelerates Flowering and Regulates Lipid Homeostasis between Oil Accumulation and Membrane Lipid Anabolism in Brassica napus. Front Plant Sci, 2015. 6: p. 1015 [PMID:26635841] Hofvander P, et al. Potato tuber expression of Arabidopsis WRINKLED1 increase triacylglycerol and membrane lipids while affecting central carbohydrate metabolism. Plant Biotechnol. J., 2016. 14(9): p. 1883-98 [PMID:26914183] Shen SL, et al. CitAP2.10 activation of the terpene synthase CsTPS1 is associated with the synthesis of (+)-valencene in 'Newhall' orange. J. Exp. Bot., 2016. 67(14): p. 4105-15 [PMID:27194737] Adhikari ND,Bates PD,Browse J WRINKLED1 Rescues Feedback Inhibition of Fatty Acid Synthesis in Hydroxylase-Expressing Seeds. Plant Physiol., 2016. 171(1): p. 179-91 [PMID:27208047] Kim MJ,Jang IC,Chua NH The Mediator Complex MED15 Subunit Mediates Activation of Downstream Lipid-Related Genes by the WRINKLED1 Transcription Factor. Plant Physiol., 2016. 171(3): p. 1951-64 [PMID:27246098] Bhattacharya S,Das N,Maiti MK Cumulative effect of heterologous AtWRI1 gene expression and endogenous BjAGPase gene silencing increases seed lipid content in Indian mustard Brassica juncea. Plant Physiol. Biochem., 2016. 107: p. 204-213 [PMID:27314514] Ma W, et al. 14-3-3 protein mediates plant seed oil biosynthesis through interaction with AtWRI1. Plant J., 2016. 88(2): p. 228-235 [PMID:27322486] Li D, et al. MYB89 Transcription Factor Represses Seed Oil Accumulation. Plant Physiol., 2017. 173(2): p. 1211-1225 [PMID:27932421] Ivarson E, et al. Effects of Overexpression of WRI1 and Hemoglobin Genes on the Seed Oil Content of Lepidium campestre. Front Plant Sci, 2016. 7: p. 2032 [PMID:28119714] An D, et al. Expression of Camelina WRINKLED1 Isoforms Rescue the Seed Phenotype of the Arabidopsis wri1 Mutant and Increase the Triacylglycerol Content in Tobacco Leaves. Front Plant Sci, 2017. 8: p. 34 [PMID:28174580] Zhai Z,Liu H,Shanklin J Phosphorylation of WRINKLED1 by KIN10 Results in Its Proteasomal Degradation, Providing a Link between Energy Homeostasis and Lipid Biosynthesis. Plant Cell, 2017. 29(4): p. 871-889 [PMID:28314829] Jin J, et al. Transcriptome and functional analysis reveals hybrid vigor for oil biosynthesis in oil palm. Sci Rep, 2017. 7(1): p. 439 [PMID:28348403] Kong Q, et al. The Arabidopsis WRINKLED1 transcription factor affects auxin homeostasis in roots. J. Exp. Bot., 2017. 68(16): p. 4627-4634 [PMID:28981783] Kang NK, et al. Increased lipid production by heterologous expression of AtWRI1 transcription factor in Nannochloropsis salina. Biotechnol Biofuels, 2017. 10: p. 231 [PMID:29046718] Pellaud S, et al. WRINKLED1 and ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE1 regulate tocochromanol metabolism in Arabidopsis. New Phytol., 2018. 217(1): p. 245-260 [PMID:29105089] Hanano A,Almousally I,Shaban M,Murphy DJ Arabidopsis plants exposed to dioxin result in a WRINKLED seed phenotype due to 20S proteasomal degradation of WRI1. J. Exp. Bot., 2018. 69(7): p. 1781-1794 [PMID:29394403]