PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Lsa002012
Organism
Lactuca sativa
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae; Lactucinae; Lactuca
Family ARF
Protein Properties Length: 799aa    MW: 88980.6 Da    PI: 6.2103
Description ARF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
PUT-187a-Lactuca_sativa-38889PU_refplantGDBView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1B373.91.9e-23127228199
                EEEE-..-HHHHTT-EE--HHH.HTT.......---..--SEEEEEETTS-EEEEEE..EEETTEEEE-TTHHHHHHHHT--TT-EEEEEE-SSSEE..E CS
         B3   1 ffkvltpsdvlksgrlvlpkkfaeeh.......ggkkeesktltledesgrsWevkliyrkksgryvltkGWkeFvkangLkegDfvvFkldgrsefelv 93 
                f+k+lt sd++++g +++p++ ae+        +++++  ++l+ +d++  +W++++i+r++++r++lt+GW+ Fv+a++L +gD+v+F   ++++ +l+
  Lsa002012 127 FCKTLTASDTSTHGGFSVPRRAAEKVfppldfsQQPPA--QELIARDLHDVEWKFRHIFRGQPKRHLLTTGWSVFVSAKRLVAGDSVLFI--WNEKNQLL 222
                99*****************************8544444..48************************************************..8899999* PP

                EEEE-S CS
         B3  94 vkvfrk 99 
                ++++r+
  Lsa002012 223 LGIRRA 228
                ***997 PP
2Auxin_resp114.41e-37253336183
  Auxin_resp   1 aahaastksvFevvYnPrastseFvvkvekvekalk.vkvsvGmRfkmafetedsserrlsGtvvgvsdldpvrWpnSkWrsLk 83 
                 aahaa+t+s F+++YnPras+se v++++k++ka++ ++vsvGmRf+m fete+ss rr++Gt++g+ dldp+rWpnS+Wrs+k
   Lsa002012 253 AAHAAATNSCFTIFYNPRASPSEIVIPLSKYVKAVYhTRVSVGMRFRMLFETEESSVRRYMGTITGIGDLDPTRWPNSHWRSVK 336
                 79*********************************9789******************************************985 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF1019364.45E-49110256IPR015300DNA-binding pseudobarrel domain
Gene3DG3DSA:2.40.330.104.5E-42120242IPR015300DNA-binding pseudobarrel domain
CDDcd100172.33E-21126227No hitNo description
PROSITE profilePS5086312.379127229IPR003340B3 DNA binding domain
SMARTSM010193.1E-24127229IPR003340B3 DNA binding domain
PfamPF023621.1E-21127228IPR003340B3 DNA binding domain
PfamPF065079.4E-33253336IPR010525Auxin response factor
PfamPF023092.0E-8669760IPR033389AUX/IAA domain
SuperFamilySSF542773.01E-7671747No hitNo description
PROSITE profilePS5174523.828672756IPR000270PB1 domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009725Biological Processresponse to hormone
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0005515Molecular Functionprotein binding
Sequence ? help Back to Top
Protein Sequence    Length: 799 aa     Download sequence    Send to blast
MKLSTSGSGQ QPQEGEKKSL NSELWHACAG PLVSLPTVGS RVIYFPQGHS EQVAATTNKE  60
IDSHIPNYPS LPPQLICQLH NVTMHADVET DEVYAQMTLQ PLTPQEQKDT FLPVELGIPS  120
RQPTNYFCKT LTASDTSTHG GFSVPRRAAE KVFPPLDFSQ QPPAQELIAR DLHDVEWKFR  180
HIFRGQPKRH LLTTGWSVFV SAKRLVAGDS VLFIWNEKNQ LLLGIRRASR PQTVMPSSVL  240
SSDSMHIGLL AAAAHAAATN SCFTIFYNPR ASPSEIVIPL SKYVKAVYHT RVSVGMRFRM  300
LFETEESSVR RYMGTITGIG DLDPTRWPNS HWRSVKVGWD ESTAGERQPR VSLWEIEPLT  360
TFPMYPSLFP LRLKRPWYPG ASSFQGDSRD DAVNGMAWLR GETGEQALNS RNFQSGGMFP  420
WMQQRVDPSF LQTNLNHQYQ AALAAGLQGV GSGDALKQQL LQFQQPVQYL QHSTHQSMSV  480
IPQQRVDPSF LHSHGQMLEN LTRSTQNQES QQHQQNPYTE TYNMMQSSIP SPSFPKTDFM  540
DPKFTSNIAP PPTMLGSDPN FSRTGQGQSQ TQSWVSKFGQ PQVNTCSTSP FQGKDGGSSN  600
SIDQESSSLD ATQNQNLFNQ INIDSSGLLL PTIDTTDLAT MPYYMQDSSE LLTPTGGGGG  660
GQIDQTTPNR NRTFVKVYKS GCVGRSLDMS RFNSYHELRE ELGEMFGIEG LLEDPQRSGW  720
QLVFVDREND VLLLGDDPWE AFVNSVWYIK ILSPEDVQQL GKQEVESLGG GQNSGERIHR  780
DHLSLSGLPP SMGSGSLDF
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4ldu_A1e-159135737388Auxin response factor 5
Search in ModeBase
Functional Description ? help Back to Top
Source Description
UniProtAuxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Regulates both stamen and gynoecium maturation. Promotes jasmonic acid production. Partially redundant with ARF6. Involved in fruit initiation. Acts as an inhibitor to stop further carpel development in the absence of fertilization and the generation of signals required to initiate fruit and seed development. {ECO:0000269|PubMed:12036261, ECO:0000269|PubMed:16107481, ECO:0000269|PubMed:16829592}.
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Repressed by miR167. {ECO:0000269|PubMed:17021043}.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_023747631.10.0auxin response factor 8-like
SwissprotQ9FGV10.0ARFH_ARATH; Auxin response factor 8
TrEMBLA0A2J6JKE60.0A0A2J6JKE6_LACSA; Auxin response factor
STRINGXP_009789648.10.0(Nicotiana sylvestris)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT5G37020.10.0auxin response factor 8
Publications ? help Back to Top
  1. Liu N, et al.
    Down-regulation of AUXIN RESPONSE FACTORS 6 and 8 by microRNA 167 leads to floral development defects and female sterility in tomato.
    J. Exp. Bot., 2014. 65(9): p. 2507-20
    [PMID:24723401]
  2. Minato N, et al.
    The phytoplasmal virulence factor TENGU causes plant sterility by downregulating of the jasmonic acid and auxin pathways.
    Sci Rep, 2014. 4: p. 7399
    [PMID:25492247]
  3. Ortega-Amaro MA, et al.
    Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance.
    Front Plant Sci, 2014. 5: p. 782
    [PMID:25653657]
  4. Wang Y, et al.
    MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development.
    Plant Physiol., 2015. 168(3): p. 984-99
    [PMID:25941314]
  5. Barik S, et al.
    Coevolution Pattern and Functional Conservation or Divergence of miR167s and their targets across Diverse Plant Species.
    Sci Rep, 2015. 5: p. 14611
    [PMID:26459056]
  6. Pashkovskiy PP,Kartashov AV,Zlobin IE,Pogosyan SI,Kuznetsov VV
    Blue light alters miR167 expression and microRNA-targeted auxin response factor genes in Arabidopsis thaliana plants.
    Plant Physiol. Biochem., 2016. 104: p. 146-54
    [PMID:27031426]
  7. Mlotshwa S,Pruss GJ,MacArthur JL,Reed JW,Vance V
    Developmental Defects Mediated by the P1/HC-Pro Potyviral Silencing Suppressor Are Not Due to Misregulation of AUXIN RESPONSE FACTOR 8.
    Plant Physiol., 2016. 172(3): p. 1853-1861
    [PMID:27688620]
  8. Wójcikowska B,Gaj MD
    Expression profiling of AUXIN RESPONSE FACTOR genes during somatic embryogenesis induction in Arabidopsis.
    Plant Cell Rep., 2017. 36(6): p. 843-858
    [PMID:28255787]
  9. Zhang GZ, et al.
    Ectopic expression of UGT84A2 delayed flowering by indole-3-butyric acid-mediated transcriptional repression of ARF6 and ARF8 genes in Arabidopsis.
    Plant Cell Rep., 2017. 36(12): p. 1995-2006
    [PMID:29027578]
  10. Zheng K, et al.
    Involvement of PACLOBUTRAZOL RESISTANCE6/KIDARI, an Atypical bHLH Transcription Factor, in Auxin Responses in Arabidopsis.
    Front Plant Sci, 2017. 8: p. 1813
    [PMID:29114256]
  11. Liu Z, et al.
    ARF2-ARF4 and ARF5 are Essential for Female and Male Gametophyte Development in Arabidopsis.
    Plant Cell Physiol., 2018. 59(1): p. 179-189
    [PMID:29145642]
  12. Ghelli R, et al.
    A Newly Identified Flower-Specific Splice Variant of AUXIN RESPONSE FACTOR8 Regulates Stamen Elongation and Endothecium Lignification in Arabidopsis.
    Plant Cell, 2018. 30(3): p. 620-637
    [PMID:29514943]