PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Lsa003004

Organism

Lactuca sativa

Taxonomic ID

4236

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae; Lactucinae; Lactuca

Family

WRKY

Protein Properties

Length: 551aa MW: 60269.5 Da PI: 6.9789

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
PUT-187a-Lactuca_sativa-32901	PU_ref	plantGDB	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	95.3	4.3e-30	214	273	1	59
---SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 1 ldDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 ++Dg++WrKYGqK kg+++pr+YYrCt + +Cpv+k+v+r+ +d +++++tYeg+Hnh+ Lsa003004 214 MNDGCQWRKYGQKIAKGNPCPRAYYRCTVSpTCPVRKQVQRCPQDMSILITTYEGTHNHP 273 58************************999**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	3.3E-31	199	273	IPR003657	WRKY domain
SuperFamily	SSF118290	1.03E-26	206	274	IPR003657	WRKY domain
PROSITE profile	PS50811	27.324	209	275	IPR003657	WRKY domain
SMART	SM00774	6.0E-36	214	274	IPR003657	WRKY domain
Pfam	PF03106	1.2E-26	215	273	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 551 aa Download sequence Send to blast
MEVDHRKTTQ MKQEDKGDDR HCKQEVVDDK EDEKQFELTK TEMGEVREEN QRLRLHLDQI 60 MKDYQNLQKK FQDILHQQQE TTSSTPHVAP TNEQPQINDE SELVSLSLGR TSSTELVKKD 120 KIRSTTPPTK NAEGLGLGLE YGKFELSSTT QTESSVNPSP ENSLDQEAKD EAGETWTPQK 180 PSTPKRSADD QDVQQNPAKK TRVSVRVRCD TPTMNDGCQW RKYGQKIAKG NPCPRAYYRC 240 TVSPTCPVRK QVQRCPQDMS ILITTYEGTH NHPLAASATA MASTTSAAAS MLTSGSSTSG 300 ANSNPNTVTT SHGLNFYLNE NSKLQPIYLP HSSISPSPSC PTVTLDLTSN TLSTSSPYSY 360 RSPPMATTFP PRYSTTNLNF SSLESNALPI SWSNGHLNYG KNQMGSLNFG STQAQENIYH 420 SYMQNKNLMA TATANQHSLQ PDTIEAATRA ITADPNFQSV LQAALTSIIG GGGVGLQGGG 480 EKSDQNIKLG EIFPVFSSFP STSNTNKCSS SFFNKSTAAT LSSQPAGSNS LPISNSRSKS 540 TSPENSRDHL V

Sequence ? help Back to Top

Protein Sequence Length: 551 aa Download sequence Send to blast

MEVDHRKTTQ MKQEDKGDDR HCKQEVVDDK EDEKQFELTK TEMGEVREEN QRLRLHLDQI  60
MKDYQNLQKK FQDILHQQQE TTSSTPHVAP TNEQPQINDE SELVSLSLGR TSSTELVKKD  120
KIRSTTPPTK NAEGLGLGLE YGKFELSSTT QTESSVNPSP ENSLDQEAKD EAGETWTPQK  180
PSTPKRSADD QDVQQNPAKK TRVSVRVRCD TPTMNDGCQW RKYGQKIAKG NPCPRAYYRC  240
TVSPTCPVRK QVQRCPQDMS ILITTYEGTH NHPLAASATA MASTTSAAAS MLTSGSSTSG  300
ANSNPNTVTT SHGLNFYLNE NSKLQPIYLP HSSISPSPSC PTVTLDLTSN TLSTSSPYSY  360
RSPPMATTFP PRYSTTNLNF SSLESNALPI SWSNGHLNYG KNQMGSLNFG STQAQENIYH  420
SYMQNKNLMA TATANQHSLQ PDTIEAATRA ITADPNFQSV LQAALTSIIG GGGVGLQGGG  480
EKSDQNIKLG EIFPVFSSFP STSNTNKCSS SFFNKSTAAT LSSQPAGSNS LPISNSRSKS  540
TSPENSRDHL V

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
1wj2_A	4e-20	214	276	17	78	Probable WRKY transcription factor 4
2lex_A	4e-20	214	276	17	78	Probable WRKY transcription factor 4
Search in ModeBase

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator involved in the transcriptional regulation of terpene biosynthesis in glandular trichomes (PubMed:24884371). Binds to the promoter of the linalool synthase TPS5 and promotes TPS5 gene transactivation (PubMed:24884371). In association with WRKY72B, contributes to basal defense against root-knot nematodes (RKNs) and potato aphids, as well as Mi-1-mediated gene-for-gene resistance to these pests (PubMed:20409007). Both WRKY72A and WRKY72B are not required for gene-for-gene resistance mediated by Pto, another tomato R gene (PubMed:20409007). {ECO:0000269\|PubMed:20409007, ECO:0000269\|PubMed:24884371}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Induced by infection with the root-knot nematode (RKN) Meloidogyne incognita and potato aphids. {ECO:0000269\|PubMed:20409007}.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_023744175.1	0.0	probable WRKY transcription factor 61 isoform X1
Swissprot	A0A3Q7GFB5	1e-128	WK72A_SOLLC; WRKY transcription factor 72A
TrEMBL	A0A2J6JSU8	0.0	A0A2J6JSU8_LACSA; Uncharacterized protein
STRING	XP_010256554.1	1e-144	(Nelumbo nucifera)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT5G15130.1	4e-62	WRKY DNA-binding protein 72

Publications ? help Back to Top
Tomato Genome Consortium The tomato genome sequence provides insights into fleshy fruit evolution. Nature, 2012. 485(7400): p. 635-41 [PMID:22660326] Spyropoulou EA,Haring MA,Schuurink RC RNA sequencing on Solanum lycopersicum trichomes identifies transcription factors that activate terpene synthase promoters. BMC Genomics, 2014. 15: p. 402 [PMID:24884371]