PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Lsa013654

Organism

Lactuca sativa

Taxonomic ID

4236

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae; Lactucinae; Lactuca

Family

bHLH

Protein Properties

Length: 354aa MW: 40215.6 Da PI: 7.0118

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
PUT-187a-Lactuca_sativa-12837	PU_ref	plantGDB	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	31.1	4.3e-10	173	220	4	55
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +h+ +Er RR++i +++ L++l+P + +k Ka +L + ++Y++sLq Lsa013654 173 SHSLAERVRREKISERMKYLQDLVPGC----NKITGKAGMLDEIINYVQSLQ 220 8************************....999***************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF47459	9.29E-17	167	236	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	8.97E-10	167	224	No hit	No description
Gene3D	G3DSA:4.10.280.10	2.4E-17	169	236	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS50888	15.618	169	219	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	1.7E-7	173	220	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	3.4E-9	175	225	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 354 aa Download sequence Send to blast
MNRVSPDMLL GHNTTVTGGT DVSVFERQQA RMKWQQLQQH QHSFYNENDH QVPNPFLGLI 60 GNIKPDPGLE NGWPDFSDGC DELFSPLMAD QRIMQLQKNS SSVLLSPKKR KTHENQKNFQ 120 VVSEVNGIKE KKLKGCSEEN SSETSSKEKS KPTEAQKQDY IHVRARRGQA TDSHSLAERV 180 RREKISERMK YLQDLVPGCN KITGKAGMLD EIINYVQSLQ KQVEFLSMKL STVHPRLDLD 240 VDNLIMKEMF EVSTAGYTSE MANSPYFQLN SLLEMGINPI DMVLRRSISG GAPVSIPETF 300 VVSSCFKQIH PNVAWDSDLQ NLYRMEFQQG SSTIPYQSQK ITGLYEGSNM KMEM

Sequence ? help Back to Top

Protein Sequence Length: 354 aa Download sequence Send to blast

MNRVSPDMLL GHNTTVTGGT DVSVFERQQA RMKWQQLQQH QHSFYNENDH QVPNPFLGLI  60
GNIKPDPGLE NGWPDFSDGC DELFSPLMAD QRIMQLQKNS SSVLLSPKKR KTHENQKNFQ  120
VVSEVNGIKE KKLKGCSEEN SSETSSKEKS KPTEAQKQDY IHVRARRGQA TDSHSLAERV  180
RREKISERMK YLQDLVPGCN KITGKAGMLD EIINYVQSLQ KQVEFLSMKL STVHPRLDLD  240
VDNLIMKEMF EVSTAGYTSE MANSPYFQLN SLLEMGINPI DMVLRRSISG GAPVSIPETF  300
VVSSCFKQIH PNVAWDSDLQ NLYRMEFQQG SSTIPYQSQK ITGLYEGSNM KMEM

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	108	112	KRKTH

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor that binds DNA to G box 5'-CACGTG-3' and, to a lower extent, to E-box 5'-CANNTG-3' in vitro. Binds to chromatin DNA of the FT gene and promotes its expression, and thus triggers flowering in response to blue light. {ECO:0000269\|PubMed:18988809, ECO:0000269\|PubMed:24130508}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Accumulates strongly in response to blue light due to reduced preventing 26S proteasome-mediated degradation in an ADO1/ZTL and ADO2/LKP2 dependent manner, but levels decrease in the absence of blue light via 26S proteasome degradation (at protein level). {ECO:0000269\|PubMed:24101505}.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_023747479.1	0.0	transcription factor bHLH63-like isoform X1
Swissprot	Q8GY61	2e-55	BH063_ARATH; Transcription factor bHLH63
TrEMBL	A0A2J6JKN0	0.0	A0A2J6JKN0_LACSA; Uncharacterized protein
STRING	XP_008236908.1	1e-114	(Prunus mume)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G34530.1	2e-56	cryptochrome-interacting basic-helix-loop-helix 1

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Yang D,Zhao W,Meng Y,Li H,Liu B A CIB1-LIKE transcription factor GmCIL10 from soybean positively regulates plant flowering. Sci China Life Sci, 2015. 58(3): p. 261-9 [PMID:25651969] Katsura Y, et al. An optogenetic system for interrogating the temporal dynamics of Akt. Sci Rep, 2015. 5: p. 14589 [PMID:26423353] Taslimi A, et al. Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. Nat. Chem. Biol., 2016. 12(6): p. 425-30 [PMID:27065233] Liu Q, et al. The Blue Light-Dependent Polyubiquitination and Degradation of Arabidopsis Cryptochrome2 Requires Multiple E3 Ubiquitin Ligases. Plant Cell Physiol., 2016. 57(10): p. 2175-2186 [PMID:27516416] Pathak GP, et al. Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res., 2017. 45(20): p. e167 [PMID:28431041] Duan L, et al. Understanding CRY2 interactions for optical control of intracellular signaling. Nat Commun, 2017. 8(1): p. 547 [PMID:28916751] Quejada JR, et al. Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1. Nucleic Acids Res., 2017. 45(20): p. e172 [PMID:29040770] Liu Y, et al. CIB1 and CO interact to mediate CRY2-dependent regulation of flowering. EMBO Rep., 2019. [PMID:30126927]