PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

MDP0000318951

Organism

Malus domestica

Taxonomic ID

3750

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae; Maloideae; Maleae; Malus

Family

bHLH

Protein Properties

Length: 378aa MW: 42303.4 Da PI: 8.5443

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
MDP0000318951	genome	GDR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	30.7	5.5e-10	114	163	8	55
HHHHHHHHHHHHHHHHHCTSCC.C....TTS-STCHHHHHHHHHHHHHHH CS HLH 8 rErrRRdriNsafeeLrellPk.as.kapskKlsKaeiLekAveYIksLq 55 Er+RR r+++++ Lr+l+P+ + + ++Ka+i++ Av Y+++Lq MDP0000318951 114 SERKRRGRMKERLYALRSLVPNiTKvSVGTVEMDKASIVRDAVLYVQDLQ 163 6*******************7665666666***************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50888	14.176	106	162	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	9.68E-14	108	177	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	5.7E-14	108	179	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	1.84E-10	108	163	No hit	No description
SMART	SM00353	6.7E-9	112	168	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	1.7E-7	114	163	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 378 aa Download sequence Send to blast
MDALGNHLEC HNIDSSELHD FIDDANLDQF IDLIRGGEGE DPIANFDCEL MINGCLDDYN 60 LFGPASTTPP SPMFGFNDAF LPDPSTFVSE TTNTNTTTTT TKRQKVDRSR TLVSERKRRG 120 RMKERLYALR SLVPNITKVS VGTVEMDKAS IVRDAVLYVQ DLQMHAKKLN AEIANLEASL 180 AGGYLQGSTK TKNKKKVSDN NHLASKGIIQ IDVSQVEEKG FYVKVXCNKG QVVATALYKA 240 LESLARFNVQ SSNLNTVSAD RFEFAFTLNV CKGLRAGHHQ LAKFETLGGW GFSEPRLCNL 300 RIAKSSSYRG CSHRRAKNAC IACMHVDNRH VKFVRRTDFL TKRLXTEWDA SSVKFFIVPX 360 AIEDLEFNVI CVYLPKSK

Sequence ? help Back to Top

Protein Sequence Length: 378 aa Download sequence Send to blast

MDALGNHLEC HNIDSSELHD FIDDANLDQF IDLIRGGEGE DPIANFDCEL MINGCLDDYN  60
LFGPASTTPP SPMFGFNDAF LPDPSTFVSE TTNTNTTTTT TKRQKVDRSR TLVSERKRRG  120
RMKERLYALR SLVPNITKVS VGTVEMDKAS IVRDAVLYVQ DLQMHAKKLN AEIANLEASL  180
AGGYLQGSTK TKNKKKVSDN NHLASKGIIQ IDVSQVEEKG FYVKVXCNKG QVVATALYKA  240
LESLARFNVQ SSNLNTVSAD RFEFAFTLNV CKGLRAGHHQ LAKFETLGGW GFSEPRLCNL  300
RIAKSSSYRG CSHRRAKNAC IACMHVDNRH VKFVRRTDFL TKRLXTEWDA SSVKFFIVPX  360
AIEDLEFNVI CVYLPKSK

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed in roots and inflorescence, and to a lower extent, in leaves and stems. In roots, confined to the outer cell layers, specifically in the differentiation zone. Also detected in the endodermis and inner tissues of the central cylinder. {ECO:0000269\|PubMed:12679534, ECO:0000269\|PubMed:15539473, ECO:0000269\|PubMed:15556641}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor. Essential protein involved in iron uptake responses. Regulates FRO2 at the level of mRNA accumulation and IRT1 at the level of protein accumulation. Confers enhanced iron mobilization responses at low iron supply. {ECO:0000269\|PubMed:15539473, ECO:0000269\|PubMed:15556641, ECO:0000269\|PubMed:16117851}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: In roots by iron deficiency. Repressed by cytokinins. Induced by cold, UV, ethylene (ACC), jasmonic acid (JA), flagellin, and salicylic acid (SA) treatments. {ECO:0000269\|PubMed:12679534, ECO:0000269\|PubMed:15531708, ECO:0000269\|PubMed:15539473, ECO:0000269\|PubMed:15556641, ECO:0000269\|PubMed:18397377}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	HQ889726	0.0	HQ889726.1 Malus xiaojinensis bHLH01 (bHLH01) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_008377907.2	0.0	transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR-like isoform X1
Swissprot	Q0V7X4	1e-68	FIT_ARATH; Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR
TrEMBL	A0A498K8R8	1e-179	A0A498K8R8_MALDO; Uncharacterized protein
STRING	XP_008377907.1	1e-180	(Malus domestica)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF3679	33	58

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT2G28160.1	4e-68	FER-like regulator of iron uptake

Link Out ? help Back to Top
Phytozome	MDP0000318951

Publications ? help Back to Top
Schmid NB, et al. Feruloyl-CoA 6'-Hydroxylase1-dependent coumarins mediate iron acquisition from alkaline substrates in Arabidopsis. Plant Physiol., 2014. 164(1): p. 160-72 [PMID:24246380] Doustaly F, et al. Uranium perturbs signaling and iron uptake response in Arabidopsis thaliana roots. Metallomics, 2014. 6(4): p. 809-21 [PMID:24549117] Le CT, et al. ZINC FINGER OF ARABIDOPSIS THALIANA12 (ZAT12) Interacts with FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT) Linking Iron Deficiency and Oxidative Stress Responses. Plant Physiol., 2016. 170(1): p. 540-57 [PMID:26556796] Yang L, et al. Carbon Monoxide Interacts with Auxin and Nitric Oxide to Cope with Iron Deficiency in Arabidopsis. Front Plant Sci, 2016. 7: p. 112 [PMID:27014280] Zhou C, et al. Paenibacillus polymyxa BFKC01 enhances plant iron absorption via improved root systems and activated iron acquisition mechanisms. Plant Physiol. Biochem., 2016. 105: p. 162-173 [PMID:27105423] Mai HJ,Pateyron S,Bauer P Iron homeostasis in Arabidopsis thaliana: transcriptomic analyses reveal novel FIT-regulated genes, iron deficiency marker genes and functional gene networks. BMC Plant Biol., 2016. 16(1): p. 211 [PMID:27716045] Zhou C, et al. Exogenous Melatonin Improves Plant Iron Deficiency Tolerance via Increased Accumulation of Polyamine-Mediated Nitric Oxide. Int J Mol Sci, 2017. [PMID:27792144] Ezer D, et al. The G-Box Transcriptional Regulatory Code in Arabidopsis. Plant Physiol., 2017. 175(2): p. 628-640 [PMID:28864470] Naranjo-Arcos MA, et al. Dissection of iron signaling and iron accumulation by overexpression of subgroup Ib bHLH039 protein. Sci Rep, 2017. 7(1): p. 10911 [PMID:28883478] Kailasam S,Wang Y,Lo JC,Chang HF,Yeh KC S-Nitrosoglutathione works downstream of nitric oxide to mediate iron-deficiency signaling in Arabidopsis. Plant J., 2018. 94(1): p. 157-168 [PMID:29396986] Cui Y, et al. Four IVa bHLH Transcription Factors Are Novel Interactors of FIT and Mediate JA Inhibition of Iron Uptake in Arabidopsis. Mol Plant, 2018. 11(9): p. 1166-1183 [PMID:29960107]