PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID MDP0000319634
Organism
Malus domestica
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae; Maloideae; Maleae; Malus
Family MYB
Protein Properties Length: 484aa    MW: 53365.9 Da    PI: 7.5948
Description MYB family protein
Gene Model
Gene Model ID Type Source Coding Sequence
MDP0000319634genomeGDRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1Myb_DNA-binding39.71.2e-12173218146
                      TSSS-HHHHHHHHHHHHHTTTT-HHHHHHHHTTTS-HHHHHHHHHH CS
  Myb_DNA-binding   1 rgrWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrwqk 46 
                      +g+W +eEde l++ vk+ G+++W++I ++  + Rt+k+c++rw +
    MDP0000319634 173 KGPWKAEEDEVLLKHVKKCGPRDWSSIRSKGLLQRTGKSCRLRWVN 218
                      79*************************8887799**********87 PP
2Myb_DNA-binding40.94.9e-13229268344
                      SS-HHHHHHHHHHHHHTTTT-HHHHHHHHTTTS-HHHHHHHH CS
  Myb_DNA-binding   3 rWTteEdellvdavkqlGggtWktIartmgkgRtlkqcksrw 44 
                      +++ eE+   +++ +q+G++ W++Ia +++ gRt++++k++w
    MDP0000319634 229 KFSVEEERVVIELQAQFGNK-WARIATYLP-GRTDNDVKNFW 268
                      799*****************.*********.*********** PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:1.10.10.605.5E-20167221IPR009057Homeodomain-like
PROSITE profilePS5129420.57168224IPR017930Myb domain
SuperFamilySSF466891.21E-27170268IPR009057Homeodomain-like
SMARTSM007172.6E-10172222IPR001005SANT/Myb domain
CDDcd001678.42E-11175220No hitNo description
PfamPF139211.5E-11176226No hitNo description
Gene3DG3DSA:1.10.10.602.1E-17222274IPR009057Homeodomain-like
PROSITE profilePS5129418.315225276IPR017930Myb domain
SMARTSM007174.4E-12226274IPR001005SANT/Myb domain
PfamPF002492.3E-11229268IPR001005SANT/Myb domain
CDDcd001674.02E-10229268No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0003677Molecular FunctionDNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 484 aa     Download sequence    Send to blast
METAVAVGPR PTCGGRCHAA QLGWAVMSSR KACAGNSFTM PFKAFAVASL YIGSIATAGV  60
AGIRKAGDLV ELGANLRTGL GISSLVANGE KYKPNGYLLV ICYRRIVDGF VIHHLFLLLF  120
SNIAFTACQG KYGDEGMALX ASLLAGVTFL ASXNFSAVFL VELKSKKEEG IRKGPWKAEE  180
DEVLLKHVKK CGPRDWSSIR SKGLLQRTGK SCRLRWVNKL RPNLKNGCKF SVEEERVVIE  240
LQAQFGNKWA RIATYLPGRT DNDVKNFWSS RQKRLARILQ TSAAAPATPS SKSHKTKXEV  300
SXFXELPTIV EATKFNCXSE GEPSSQAQPC LSXPPYXRNS EIIKMVPLPD IVKPKSLCFE  360
TNPVQHELPI TDKDPWTDSH SVIPFLQNPQ PQTDQIFSSE TQEFLARFDP YFDMLGPLDA  420
LELGNGGEPL VMEPPLFEPV GSCLSCEXEK IDHTMTPDCV FDDFPTDMFD QFDPLPNTSG  480
SGQH
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1h8a_C3e-2817127425126MYB TRANSFORMING PROTEIN
Search in ModeBase
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Strict spatial and temporal transcriptional activation leading to a specific expression in the male germline cells; this specific expression pattern is dependent of the regulatory region of DUO1 (ROD1) 5'-YAACYGY-3' in its promoter (PubMed:15694308, PubMed:27624837). First observed in the germ cell during or soon after engulfment by the vegetative cell, just after asymmetric division. In maturating pollen, accumulates in germ cells before mitosis and remains high in mature sperm cells. Expression persists during pollen development (PubMed:19300502). {ECO:0000269|PubMed:15694308, ECO:0000269|PubMed:19300502, ECO:0000269|PubMed:27624837}.
UniprotTISSUE SPECIFICITY: Confined to inflorescences, especially in stamens and pollen. {ECO:0000269|PubMed:15694308}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription activator that acts as a positive regulator of male germline development by promoting both gametic cell specification and cell cycle progression (PubMed:15694308, PubMed:15618418, PubMed:19300502, PubMed:21285328). Binds to canonical MYB sites 5'-AACCGTC-3', 5'-AAACCGC-3' and 5'-AACCGT-3' in promoters to trigger the expression of male germline-specific or enriched genes (e.g. MGH3, GEX2 and GCS1), including those required for fertilization (PubMed:21285328, PubMed:19300502). Required for sperm cell specification leading to pollen maturation by activating a germline-specific regulon (PubMed:21285328, PubMed:15694308, PubMed:15618418, PubMed:19300502). Involved in pollen mitosis entry at G2-M transition via the regulation of CYCB1-1, DAZ1 and DAZ2 expression (PubMed:15618418, PubMed:19300502, PubMed:24876252). {ECO:0000269|PubMed:15618418, ECO:0000269|PubMed:15694308, ECO:0000269|PubMed:19300502, ECO:0000269|PubMed:21285328, ECO:0000269|PubMed:24876252}.
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Repressed by the microRNA 159 (miR159); the production of miR159 is stimulated by the anaphase promoting complex/cyclosome (APC/C) (PubMed:21441434). Activated by ARID1 in male germline cells via specific histone acetylation regulation (e.g. H3K9Ac) (PubMed:25057814). {ECO:0000269|PubMed:21441434, ECO:0000269|PubMed:25057814}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_009343900.10.0PREDICTED: transcription factor MYB24-like
SwissprotA0A178VEK71e-84DUO1_ARATH; Transcription factor DUO1
TrEMBLA0A498JC960.0A0A498JC96_MALDO; Uncharacterized protein
STRINGXP_008375755.10.0(Malus domestica)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
FabidsOGEF36933164
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT3G60460.16e-86MYB family protein
Publications ? help Back to Top
  1. Zheng B,He H,Zheng Y,Wu W,McCormick S
    An ARID domain-containing protein within nuclear bodies is required for sperm cell formation in Arabidopsis thaliana.
    PLoS Genet., 2014. 10(7): p. e1004421
    [PMID:25057814]
  2. Peters B, et al.
    A Conserved cis-Regulatory Module Determines Germline Fate through Activation of the Transcription Factor DUO1 Promoter.
    Plant Physiol., 2017. 173(1): p. 280-293
    [PMID:27624837]
  3. Zheng Z, et al.
    Target RNA Secondary Structure Is a Major Determinant of miR159 Efficacy.
    Plant Physiol., 2017. 174(3): p. 1764-1778
    [PMID:28515145]