PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

MLOC_36351.2

Organism

Hordeum vulgare

Taxonomic ID

4513

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum

Family

bHLH

Protein Properties

Length: 245aa MW: 26873.4 Da PI: 7.2664

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
MLOC_36351.2	genome	IBSC	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	43	8e-14	70	121	1	55
CHHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 1 rrrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 r+ +hn+ Er RR+++N+ ++ Lr+llP + +++Kls ++ ++ +YI +Lq MLOC_36351.2 70 RKISHNAYERDRRKQLNELYSDLRSLLPDS---DHTKLSIPITVSRVLKYIPELQ 121 6889************************...9****************998 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF47459	4.06E-16	68	137	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS50888	13.548	69	120	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	2.53E-10	69	124	No hit	No description
Pfam	PF00010	5.5E-11	70	121	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	1.1E-12	70	136	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	9.1E-9	75	126	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 245 aa Download sequence Send to blast
MGHQTQMFDD PFASSMSSLD ADIFSVAGGL HPSQWPGLDH DVSLAPAANN GTSSGGYGSP 60 GGGDGSGSHR KISHNAYERD RRKQLNELYS DLRSLLPDSD HTKLSIPITV SRVLKYIPEL 120 QKQVDGLEKK KEELTRASCK PGVLTMKENT VPIVSATCLD EREIMVQVSL VSTMAGALPM 180 SKCIKVLENE GLRLISSSTS AFQNRTFYSL HLQRTQRTMS KECPAFCEEL ENALTQKAGL 240 RLHHQ

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator that binds to the DNA motif 5'-CACGTGG-3' in the promoter of iron (Fe) deficiency-inducible genes as well as of genes involved in iron homeostasis, thus contributing to basal tolerance to iron deficiency, iron uptake from soil and iron transport, particularly during seed maturation and germination (PubMed:16887895, PubMed:17559517, PubMed:21331630, PubMed:26224556). Promotes the accumulation of mugineic acid family phytosiderophores (MAs) (PubMed:17559517). Required for ethylene-mediated signaling during iron deficiency responses (PubMed:21112958). Improves growth and yield, especially in calcareous soil with low iron availability. Promotes iron concentration in shoots and grain (PubMed:21331630). {ECO:0000269\|PubMed:16887895, ECO:0000269\|PubMed:17559517, ECO:0000269\|PubMed:21112958, ECO:0000269\|PubMed:21331630, ECO:0000269\|PubMed:26224556}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	MLOC_36351.2

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Strongly induced in both roots and shoots during iron (Fe) deficiency stress (PubMed:16887895, PubMed:18025467, PubMed:19737364, PubMed:21112958, PubMed:21331630, PubMed:24280375). Ethylene enhances the iron deficiency-mediated induction (PubMed:21112958). Induced by arsenate (AsV25 and AsV50); this induction is repressed by nitric oxide (NO) (PubMed:26793232). Accumulates under cadmium (Cd) stress; this induction is inhibited by the DNA methyltransferase inhibitor 5-aza-2-deoxycytidine (Aza) (PubMed:27412910). {ECO:0000269\|PubMed:16887895, ECO:0000269\|PubMed:18025467, ECO:0000269\|PubMed:19737364, ECO:0000269\|PubMed:21112958, ECO:0000269\|PubMed:21331630, ECO:0000269\|PubMed:24280375, ECO:0000269\|PubMed:26793232, ECO:0000269\|PubMed:27412910}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK251746	0.0	AK251746.1 Hordeum vulgare subsp. vulgare cDNA clone: FLbaf134m16, mRNA sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_020193397.1	1e-153	transcription factor bHLH101-like
Swissprot	Q0JFZ0	9e-96	IRO2_ORYSJ; Protein IRON-RELATED TRANSCRIPTION FACTOR 2
TrEMBL	M0VQ63	0.0	M0VQ63_HORVV; Uncharacterized protein
STRING	MLOC_36351.1	1e-165	(Hordeum vulgare)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G56970.1	7e-30	bHLH family protein

Publications ? help Back to Top
Ogo Y, et al. The rice bHLH protein OsIRO2 is an essential regulator of the genes involved in Fe uptake under Fe-deficient conditions. Plant J., 2007. 51(3): p. 366-77 [PMID:17559517] Kobayashi T, et al. The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes. Plant J., 2009. 60(6): p. 948-61 [PMID:19737364] Wu J, et al. Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa. J. Exp. Bot., 2011. 62(2): p. 667-74 [PMID:21112958] Ogo Y, et al. OsIRO2 is responsible for iron utilization in rice and improves growth and yield in calcareous soil. Plant Mol. Biol., 2011. 75(6): p. 593-605 [PMID:21331630] Yang A,Li Y,Xu Y,Xu Y,Zhang WH A receptor-like protein RMC is involved in regulation of iron acquisition in rice. J. Exp. Bot., 2013. 64(16): p. 5009-20 [PMID:24014863] Itai RN,Ogo Y,Kobayashi T,Nakanishi H,Nishizawa NK Rice genes involved in phytosiderophore biosynthesis are synchronously regulated during the early stages of iron deficiency in roots. Rice (N Y), 2013. 6(1): p. 16 [PMID:24280375] Masuda H,Aung MS,Nishizawa NK Iron biofortification of rice using different transgenic approaches. Rice (N Y), 2013. 6(1): p. 40 [PMID:24351075] Kobayashi T,Nakanishi Itai R,Nishizawa NK Iron deficiency responses in rice roots. Rice (N Y), 2014. 7(1): p. 27 [PMID:26224556] Singh AP, et al. Nitric Oxide Alleviated Arsenic Toxicity by Modulation of Antioxidants and Thiol Metabolism in Rice (Oryza sativa L.). Front Plant Sci, 2015. 6: p. 1272 [PMID:26793232] Yang A,Zhang WH A Small GTPase, OsRab6a, is Involved in the Regulation of Iron Homeostasis in Rice. Plant Cell Physiol., 2016. 57(6): p. 1271-80 [PMID:27257291] Feng SJ, et al. Variation of DNA methylation patterns associated with gene expression in rice (Oryza sativa) exposed to cadmium. Plant Cell Environ., 2016. 39(12): p. 2629-2649 [PMID:27412910]