PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

MLOC_60291.1

Organism

Hordeum vulgare

Taxonomic ID

4513

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum

Family

bHLH

Protein Properties

Length: 419aa MW: 45316.4 Da PI: 4.9544

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
MLOC_60291.1	genome	IBSC	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	41.6	2.2e-13	188	239	1	55
CHHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 1 rrrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +r++h ++Er+RR+++N+ + Lr+l+P + + ++ + a+i A+e+I++L+ MLOC_60291.1 188 QRMTHIAVERNRRRQMNDYLRVLRSLMPGS---YVQRGDQASIIGGAIEFIRELE 239 79**************************...9****************996 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:4.10.280.10	1.4E-13	186	242	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	1.74E-14	186	243	No hit	No description
SuperFamily	SSF47459	1.31E-16	187	252	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS50888	15.859	187	238	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	9.6E-11	188	239	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	1.3E-9	193	244	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0010377	Biological Process	guard cell fate commitment
GO:0045597	Biological Process	positive regulation of cell differentiation
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:0051782	Biological Process	negative regulation of cell division
GO:0005634	Cellular Component	nucleus
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 419 aa Download sequence Send to blast
MEKQGSSHQL DTFAQLDGGA APEEPMGGGG AGAEMVDYML GQTTPLPPGG PHGQVSLDKL 60 SFSDVLQFAD FGPRLALNQP SADHDDARDD DEEDSYFFRF QSQLSGSDEP DPRGRAGIHH 120 AAEQEGSKTP DGSGGPHDHG GGVSESTTLV QQSDGGGRVG QKAGGEQVKT GRRKRPRSTK 180 TSEEVESQRM THIAVERNRR RQMNDYLRVL RSLMPGSYVQ RGDQASIIGG AIEFIRELEQ 240 LIQCLESQKR RRLYGDTPRP VADASAPVVP ATSIHEPPPQ GHEAPPFYVS PSLSFPGAAN 300 GDGAAAAKVM IDLDTCSGGG LREEVAENKS SLADIEVRVL GEDAVIKVLS RRRPEQLIKT 360 IAVLEEMHMS ILHTNITTIE QTVLYSFNVK ITSEPRFTAE EIVGAVHQIL SFIDVNYTL

Sequence ? help Back to Top

Protein Sequence Length: 419 aa Download sequence Send to blast

MEKQGSSHQL DTFAQLDGGA APEEPMGGGG AGAEMVDYML GQTTPLPPGG PHGQVSLDKL  60
SFSDVLQFAD FGPRLALNQP SADHDDARDD DEEDSYFFRF QSQLSGSDEP DPRGRAGIHH  120
AAEQEGSKTP DGSGGPHDHG GGVSESTTLV QQSDGGGRVG QKAGGEQVKT GRRKRPRSTK  180
TSEEVESQRM THIAVERNRR RQMNDYLRVL RSLMPGSYVQ RGDQASIIGG AIEFIRELEQ  240
LIQCLESQKR RRLYGDTPRP VADASAPVVP ATSIHEPPPQ GHEAPPFYVS PSLSFPGAAN  300
GDGAAAAKVM IDLDTCSGGG LREEVAENKS SLADIEVRVL GEDAVIKVLS RRRPEQLIKT  360
IAVLEEMHMS ILHTNITTIE QTVLYSFNVK ITSEPRFTAE EIVGAVHQIL SFIDVNYTL

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	188	199	RMTHIAVERNRR
2	248	252	KRRRL

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator (PubMed:17088607, PubMed:17183265, PubMed:17183267). Together with MYB88 and MYB124, ensures that stomata contain just two guard cells (GCs) by enforcing a single symmetric precursor cell division before stomatal maturity (PubMed:24571519). Together with SPCH and MUTE, regulates the stomata formation. Required to promote differentiation and morphogenesis of stomatal guard cells and to halt proliferative divisions in their immediate precursors. Mediates the formation of stomata (PubMed:17088607, PubMed:17183265, PubMed:17183267). Prevents histone H3K27me3 marks and derepresses stem cell gene expression (PubMed:24654956). {ECO:0000269\|PubMed:17088607, ECO:0000269\|PubMed:17183265, ECO:0000269\|PubMed:17183267, ECO:0000269\|PubMed:24571519, ECO:0000269\|PubMed:24654956}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	MLOC_60291.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Inhibited by low relative humidity (LRH) via epigenetic CG methylation, thus leading to a reduced stomatal index. {ECO:0000269\|PubMed:22442411}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AK252109	0.0	AK252109.1 Hordeum vulgare subsp. vulgare cDNA clone: FLbaf148l20, mRNA sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_020161726.1	0.0	transcription factor FAMA
Swissprot	Q56YJ8	1e-110	FAMA_ARATH; Transcription factor FAMA
TrEMBL	M0XI93	0.0	M0XI93_HORVV; Uncharacterized protein
STRING	MLOC_60291.1	0.0	(Hordeum vulgare)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP9502	33	40

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G24140.1	1e-105	bHLH family protein

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Matos JL, et al. Irreversible fate commitment in the Arabidopsis stomatal lineage requires a FAMA and RETINOBLASTOMA-RELATED module. Elife, 2015. [PMID:25303364] Chen L, et al. NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis. Development, 2016. 143(9): p. 1600-11 [PMID:26989174] Lee JH,Jung JH,Park CM Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis. Plant Cell, 2017. 29(11): p. 2817-2830 [PMID:29070509] Han X, et al. Jasmonate Negatively Regulates Stomatal Development in Arabidopsis Cotyledons. Plant Physiol., 2018. 176(4): p. 2871-2885 [PMID:29496884] Li X,Yang R,Chen H The Arabidopsis thaliana Mediator subunit MED8 regulates plant immunity to Botrytis Cinerea through interacting with the basic helix-loop-helix (bHLH) transcription factor FAMA. PLoS ONE, 2018. 13(3): p. e0193458 [PMID:29513733]