PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Medtr1g112680.1
Common NameMTR_1g112680
Organism
Medicago truncatula
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Trifolieae; Medicago
Family bHLH
Protein Properties Length: 522aa    MW: 59115.5 Da    PI: 4.7193
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Medtr1g112680.1genomeMtView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH36.87.1e-12320363755
                      HHHHHHHHHHHHHHHHHHCTSCC.C...TTS-STCHHHHHHHHHHHHHHH CS
              HLH   7 erErrRRdriNsafeeLrellPk.askapskKlsKaeiLekAveYIksLq 55 
                       +Er+RR+++N+++ +Lr+l+P  +      Kl+ a+iL  A+e++k+Lq
  Medtr1g112680.1 320 VAERKRRKKLNDRLYNLRSLVPRiS------KLDRASILGDAIEFVKDLQ 363
                      68*********************66......******************9 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PfamPF142151.7E-2011155IPR025610Transcription factor MYC/MYB N-terminal
CDDcd000836.18E-13312367No hitNo description
PROSITE profilePS5088815.294313362IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474597.33E-17316378IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003533.3E-15319368IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.102.7E-16320376IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000101.8E-9320363IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profilePS516718.941450521IPR002912ACT domain
CDDcd048735.89E-5451514No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0008152Biological Processmetabolic process
GO:0009555Biological Processpollen development
GO:0048657Biological Processanther wall tapetum cell differentiation
GO:0005634Cellular Componentnucleus
GO:0000978Molecular FunctionRNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0016597Molecular Functionamino acid binding
GO:0046983Molecular Functionprotein dimerization activity
Sequence ? help Back to Top
Protein Sequence    Length: 522 aa     Download sequence    Send to blast
MNNFIMQNIT ERLRPLVGLN GWDYCVYWKL SEDQRYLEWL GCCCGGSESN QNGGEEHLFP  60
VSSCRDTMFS HPRTNSCDLL SQLSTSIPID SGIHAQTLLT NQPNWLNYPN SLDPNTLHET  120
INGTHVLIPV PGGLVELFVT KQVGEDQNVI DFITSQCIVM VDQEGMNHSI GFNSMSNMQS  180
NVGDENDNTN NIFHPSETLN LPHDISVLNF MQQFNYSYNQ QQNRIKNDGT FSEEYLGPFL  240
HDHENNNTLK SNTEEEHDSK SLMTENNQYV FVDPLDTKKK QENVVGRSDS MSDCSDQNEE  300
EDDGKYRRRS GKGNQSKNLV AERKRRKKLN DRLYNLRSLV PRISKLDRAS ILGDAIEFVK  360
DLQKQVKELQ DELEENSETG TESNCVYVDG VQVKANNNNN VTKAEHGFHV GNGYVSKQKQ  420
EDVGTVVTDK QTQQMEAQVE VAQIDGNEYF VKVFCEHRPG GFVKLMDAFN TLGMDVMHAT  480
VTSHKGLVSN VFKVEKKESE IVEAEEVRDS LLELTRNKSE S*
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1306327RRRSGKGNQSKNLVAERKRRKK
2321328ERKRRKKL
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: First observed a bud stage of 0.6-mm, shortly after microspore release from the postmeiotic tetrads. Later restricted within the tapetum, microspores and anther locule. Still visible within the haploid nuclei, each of which had migrated to the pollen cell wall prior to pollen mitosis I. Not expressed later. {ECO:0000269|PubMed:12535353}.
UniprotTISSUE SPECIFICITY: Mostly expressed in closed, post-meiotic buds, and, to a lower extent, in pre-meiotic buds. Detected in leaves, stems, and flowers. {ECO:0000269|PubMed:12535353, ECO:0000269|PubMed:12679534}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription factor. Plays a crucial role in tapetum development. Required for male fertility and pollen differentiation, especially during the post-meiotic transcriptional regulation of microspore development within the developing anther (PubMed:12535353). Binds E-box regions in the AHL16/TEK promoter. {ECO:0000269|PubMed:12535353, ECO:0000269|PubMed:24804694}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapMedtr1g112680.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_013470329.10.0transcription factor ABORTED MICROSPORES
SwissprotQ9ZVX21e-147AMS_ARATH; Transcription factor ABORTED MICROSPORES
TrEMBLA0A072VRJ50.0A0A072VRJ5_MEDTR; Putative transcription factor bHLH family
STRINGXP_004497179.10.0(Cicer arietinum)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
FabidsOGEF42253247
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT2G16910.11e-124bHLH family protein
Publications ? help Back to Top
  1. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]
  2. Young ND, et al.
    The Medicago genome provides insight into the evolution of rhizobial symbioses.
    Nature, 2011. 480(7378): p. 520-4
    [PMID:22089132]
  3. Jiang J,Zhang Z,Cao J
    Pollen wall development: the associated enzymes and metabolic pathways.
    Plant Biol (Stuttg), 2013. 15(2): p. 249-63
    [PMID:23252839]
  4. Xu J, et al.
    ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis.
    Plant Cell, 2014. 26(4): p. 1544-1556
    [PMID:24781116]
  5. Qian H, et al.
    Trace concentrations of imazethapyr (IM) affect floral organs development and reproduction in Arabidopsis thaliana: IM-induced inhibition of key genes regulating anther and pollen biosynthesis.
    Ecotoxicology, 2015. 24(1): p. 163-71
    [PMID:25348600]
  6. Xiong SX, et al.
    The transcription factors MS188 and AMS form a complex to activate the expression of CYP703A2 for sporopollenin biosynthesis in Arabidopsis thaliana.
    Plant J., 2016. 88(6): p. 936-946
    [PMID:27460657]
  7. Ferguson AC, et al.
    Biphasic regulation of the transcription factor ABORTED MICROSPORES (AMS) is essential for tapetum and pollen development in Arabidopsis.
    New Phytol., 2017. 213(2): p. 778-790
    [PMID:27787905]
  8. Lou Y, et al.
    Positive regulation of AMS by TDF1 and the formation of a TDF1-AMS complex are required for anther development in Arabidopsis thaliana.
    New Phytol., 2018. 217(1): p. 378-391
    [PMID:28940573]
  9. Bi H,Fan W,Zhang P
    C4 Protein of Sweet Potato Leaf Curl Virus Regulates Brassinosteroid Signaling Pathway through Interaction with AtBIN2 and Affects Male Fertility in Arabidopsis.
    Front Plant Sci, 2017. 8: p. 1689
    [PMID:29021807]