PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Medtr2g081040.1

Common Name

MTR_2g081040

Organism

Medicago truncatula

Taxonomic ID

3880

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; Trifolieae; Medicago

Family

bHLH

Protein Properties

Length: 362aa MW: 40255.7 Da PI: 8.2175

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Medtr2g081040.1	genome	Mt	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	37	6e-12	180	222	7	54
HHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHH CS HLH 7 erErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 ++E++RR+++ + +++Lr+++Pk +K++K++iL Av+Y+k+L Medtr2g081040.1 180 IAEKKRRQKLSDNMHKLRSVVPKI-----TKMDKISILGDAVDYLKEL 222 68*******************7.....7**************99 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50888	15.072	173	222	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	1.7E-15	177	242	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	6.3E-13	179	228	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	1.34E-11	180	227	No hit	No description
Pfam	PF00010	3.7E-9	180	222	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	1.5E-14	180	235	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS51671	9.966	292	361	IPR002912	ACT domain
CDD	cd04873	2.74E-7	297	358	No hit	No description

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0008152	Biological Process	metabolic process
GO:0003677	Molecular Function	DNA binding
GO:0016597	Molecular Function	amino acid binding
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 362 aa Download sequence Send to blast
MERDMLPWFK PQIEEEWYMN NIPPCTENLI PNLQNHIQQQ NIVGSSDPNT FVFEHHLGSS 60 FSLPSPKSNF SDLLNINENI NITDTTTTNN PFGNVFNLEP QGVVQPPLSI AKLTSNYDSN 120 ESVGVGKMGN HGKNKGNNYN SNNNVGGSNN NIIYNNDDEN CVVEGVKKEK KKKTPSKSLI 180 AEKKRRQKLS DNMHKLRSVV PKITKMDKIS ILGDAVDYLK ELKKQISDLQ SEIESSSPRS 240 FVPPPAGTRI KTSTMSTLPV QMKEKLCPNN VSGLKNQPTK VDVRVREGGI VNIHMLCAYK 300 PDVLASTMKA LDSLGLDVHR ANISCFNGFS LDVFKAEQHN KDQELTPEQI EAVLLKALGF 360 H*

Sequence ? help Back to Top

Protein Sequence Length: 362 aa Download sequence Send to blast

MERDMLPWFK PQIEEEWYMN NIPPCTENLI PNLQNHIQQQ NIVGSSDPNT FVFEHHLGSS  60
FSLPSPKSNF SDLLNINENI NITDTTTTNN PFGNVFNLEP QGVVQPPLSI AKLTSNYDSN  120
ESVGVGKMGN HGKNKGNNYN SNNNVGGSNN NIIYNNDDEN CVVEGVKKEK KKKTPSKSLI  180
AEKKRRQKLS DNMHKLRSVV PKITKMDKIS ILGDAVDYLK ELKKQISDLQ SEIESSSPRS  240
FVPPPAGTRI KTSTMSTLPV QMKEKLCPNN VSGLKNQPTK VDVRVREGGI VNIHMLCAYK  300
PDVLASTMKA LDSLGLDVHR ANISCFNGFS LDVFKAEQHN KDQELTPEQI EAVLLKALGF  360
H*

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	170	185	KKKTPSKSLIAEKKRR
2	182	187	KKRRQK

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Widely expressed in the whole plant with high expression in leaves and stem. Broad expression within stomatal cell lineages of leaf epidermis. {ECO:0000269\|PubMed:18641265}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcriptional activator that regulates the cold-induced transcription of CBF/DREB1 genes. Binds specifically to the MYC recognition sites (5'-CANNTG-3') found in the CBF3/DREB1A promoter. Mediates stomatal differentiation in the epidermis probably by controlling successive roles of SPCH, MUTE, and FAMA. Functions as a dimer with SPCH during stomatal initiation (PubMed:18641265, PubMed:28507175). {ECO:0000269\|PubMed:17416732, ECO:0000269\|PubMed:18641265, ECO:0000269\|PubMed:28507175}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Medtr2g081040.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By high-salt stress, cold stress and abscisic acid (ABA) treatment.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AC126013	0.0	AC126013.12 Medicago truncatula clone mth2-32e10, complete sequence.
GenBank	AC130811	0.0	AC130811.22 Medicago truncatula clone mth2-26b4, complete sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_003603119.3	0.0	transcription factor ICE1
Swissprot	Q9LSE2	9e-65	ICE1_ARATH; Transcription factor ICE1
TrEMBL	G7IR55	0.0	G7IR55_MEDTR; Helix loop helix DNA-binding domain protein
STRING	AES69496	1e-166	(Medicago truncatula)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G26744.4	3e-66	bHLH family protein

Link Out ? help Back to Top
Phytozome	Medtr2g081040.1
Entrez Gene	11407805

Publications ? help Back to Top
Young ND, et al. The Medicago genome provides insight into the evolution of rhizobial symbioses. Nature, 2011. 480(7378): p. 520-4 [PMID:22089132] Chen Y, et al. Ambient temperature enhanced freezing tolerance of Chrysanthemum dichrum CdICE1 Arabidopsis via miR398. BMC Biol., 2013. 11: p. 121 [PMID:24350981] Xu F, et al. Increased drought tolerance through the suppression of ESKMO1 gene and overexpression of CBF-related genes in Arabidopsis. PLoS ONE, 2014. 9(9): p. e106509 [PMID:25184213] Jiang W,Wu J,Zhang Y,Yin L,Lu J Isolation of a WRKY30 gene from Muscadinia rotundifolia (Michx) and validation of its function under biotic and abiotic stresses. Protoplasma, 2015. 252(5): p. 1361-74 [PMID:25643917] Lang Z,Zhu J OST1 phosphorylates ICE1 to enhance plant cold tolerance. Sci China Life Sci, 2015. 58(3): p. 317-8 [PMID:25680856] Juan JX, et al. Agrobacterium-mediated transformation of tomato with the ICE1 transcription factor gene. Genet. Mol. Res., 2015. 14(1): p. 597-608 [PMID:25729995] Lee HG,Seo PJ The MYB96-HHP module integrates cold and abscisic acid signaling to activate the CBF-COR pathway in Arabidopsis. Plant J., 2015. 82(6): p. 962-77 [PMID:25912720] Horst RJ, et al. Molecular Framework of a Regulatory Circuit Initiating Two-Dimensional Spatial Patterning of Stomatal Lineage. PLoS Genet., 2015. 11(7): p. e1005374 [PMID:26203655] Lee JH,Jung JH,Park CM INDUCER OF CBF EXPRESSION 1 integrates cold signals into FLOWERING LOCUS C-mediated flowering pathways in Arabidopsis. Plant J., 2015. 84(1): p. 29-40 [PMID:26248809] Wang CL,Zhang SC,Qi SD,Zheng CC,Wu CA Delayed germination of Arabidopsis seeds under chilling stress by overexpressing an abiotic stress inducible GhTPS11. Gene, 2016. 575(2 Pt 1): p. 206-12 [PMID:26325072] Lee JH,Park CM Integration of photoperiod and cold temperature signals into flowering genetic pathways in Arabidopsis. Plant Signal Behav, 2015. 10(11): p. e1089373 [PMID:26430754] Su F, et al. Burkholderia phytofirmans PsJN reduces impact of freezing temperatures on photosynthesis in Arabidopsis thaliana. Front Plant Sci, 2015. 6: p. 810 [PMID:26483823] Klermund C, et al. LLM-Domain B-GATA Transcription Factors Promote Stomatal Development Downstream of Light Signaling Pathways in Arabidopsis thaliana Hypocotyls. Plant Cell, 2016. 28(3): p. 646-60 [PMID:26917680] Chen L, et al. NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis. Development, 2016. 143(9): p. 1600-11 [PMID:26989174] Lu X, et al. A novel Zea mays ssp. mexicana L. MYC-type ICE-like transcription factor gene ZmmICE1, enhances freezing tolerance in transgenic Arabidopsis thaliana. Plant Physiol. Biochem., 2017. 113: p. 78-88 [PMID:28189052] Deng C,Ye H,Fan M,Pu T,Yan J The rice transcription factors OsICE confer enhanced cold tolerance in transgenic Arabidopsis. Plant Signal Behav, 2017. 12(5): p. e1316442 [PMID:28414264] de Marcos A, et al. A Mutation in the bHLH Domain of the SPCH Transcription Factor Uncovers a BR-Dependent Mechanism for Stomatal Development. Plant Physiol., 2017. 174(2): p. 823-842 [PMID:28507175] Kim SH, et al. Phosphorylation of the transcriptional repressor MYB15 by mitogen-activated protein kinase 6 is required for freezing tolerance in Arabidopsis. Nucleic Acids Res., 2017. 45(11): p. 6613-6627 [PMID:28510716] Pal S, et al. TransDetect Identifies a New Regulatory Module Controlling Phosphate Accumulation. Plant Physiol., 2017. 175(2): p. 916-926 [PMID:28827455] Zhao C, et al. MAP Kinase Cascades Regulate the Cold Response by Modulating ICE1 Protein Stability. Dev. Cell, 2017. 43(5): p. 618-629.e5 [PMID:29056551] Li H, et al. MPK3- and MPK6-Mediated ICE1 Phosphorylation Negatively Regulates ICE1 Stability and Freezing Tolerance in Arabidopsis. Dev. Cell, 2017. 43(5): p. 630-642.e4 [PMID:29056553] Lee JH,Jung JH,Park CM Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis. Plant Cell, 2017. 29(11): p. 2817-2830 [PMID:29070509] Liu Y,Zhou J MAPping Kinase Regulation of ICE1 in Freezing Tolerance. Trends Plant Sci., 2018. 23(2): p. 91-93 [PMID:29248419] Xie H, et al. Variation in ICE1 Methylation Primarily Determines Phenotypic Variation in Freezing Tolerance in Arabidopsis thaliana. Plant Cell Physiol., 2019. 60(1): p. 152-165 [PMID:30295898]