PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID NNU_000459-RA
Organism
Nelumbo nucifera
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; stem eudicotyledons; Proteales; Nelumbonaceae; Nelumbo
Family ARF
Protein Properties Length: 804aa    MW: 90220.1 Da    PI: 6.233
Description ARF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
NNU_000459-RAgenomeCASView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1B356.35.7e-18127208177
                    EEEE-..-HHHHTT-EE--HHH.HTT.......---..--SEEEEEETTS-EEEEEE..EEETTEEEE-TTHHHHHHHHT--TT CS
             B3   1 ffkvltpsdvlksgrlvlpkkfaeeh.......ggkkeesktltledesgrsWevkliyrkksgryvltkGWkeFvkangLkeg 77 
                    f+k+lt sd++++g +++p++ ae+        +++++  ++l+ +d++  +W++++i+r++++r++lt+GW+ Fv+a++L +g
  NNU_000459-RA 127 FCKTLTASDTSTHGGFSVPRRAAEKVfppldfsQQPPA--QELIARDLHDVEWKFRHIFRGQPKRHLLTTGWSVFVSAKRLVAG 208
                    99*****************************8544444..48***************************************987 PP
2Auxin_resp1201.9e-39213296183
     Auxin_resp   1 aahaastksvFevvYnPrastseFvvkvekvekalk.vkvsvGmRfkmafetedsserrlsGtvvgvsdldpvrWpnSkWrsLk 83 
                    aahaa+t+s F+++YnPras+seFv++++k++ka++ ++vsvGmRf+m fete+ss rr++Gt++g+sdldp rWpnS+Wrs+k
  NNU_000459-RA 213 AAHAAATNSRFTIFYNPRASPSEFVIPLSKFVKAVYhTRVSVGMRFRMLFETEESSVRRYMGTITGISDLDPARWPNSHWRSVK 296
                    79*********************************9789******************************************985 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF1019364.58E-39111217IPR015300DNA-binding pseudobarrel domain
Gene3DG3DSA:2.40.330.102.8E-31120208IPR015300DNA-binding pseudobarrel domain
CDDcd100173.38E-15126204No hitNo description
PfamPF023625.6E-16127208IPR003340B3 DNA binding domain
PROSITE profilePS508638.966127229IPR003340B3 DNA binding domain
SMARTSM010192.2E-13127229IPR003340B3 DNA binding domain
PfamPF065072.6E-34213296IPR010525Auxin response factor
SuperFamilySSF542776.21E-7671752No hitNo description
PfamPF023091.6E-7674765IPR033389AUX/IAA domain
PROSITE profilePS5174523.728678762IPR000270PB1 domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009733Biological Processresponse to auxin
GO:0009908Biological Processflower development
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0005515Molecular Functionprotein binding
Sequence ? help Back to Top
Protein Sequence    Length: 804 aa     Download sequence    Send to blast
MKLSTSGMVQ QGQEGQKRCL NSELWHACAG PLVSLPTVGT RVVYFPQGHS EQVAASTNKE  60
VDSHIPNYPN LPPQLICQLH DVTMHADVET DEVYAQMTLQ PLTPEEQKET YLPAELGVPS  120
KQPTNYFCKT LTASDTSTHG GFSVPRRAAE KVFPPLDFSQ QPPAQELIAR DLHDVEWKFR  180
HIFRGQPKRH LLTTGWSVFV SAKRLVAGLL AAAAHAAATN SRFTIFYNPR ASPSEFVIPL  240
SKFVKAVYHT RVSVGMRFRM LFETEESSVR RYMGTITGIS DLDPARWPNS HWRSVKVGWD  300
ESTAGERQPR VSLWEIEPLT TFPMYPSLFP LRLKRPWHPG VSSLHDNKDD VANGLMWLRG  360
ETGERGLQSL NFQALGVSPW MHQRVDTSLL GNEHDHQYHN MAAAALQDIR SGDPLKQQFL  420
QLQQPFQYLQ QSYRPNPLLQ QQQQMINQTV PQPILHQQNQ NILDSQPRHL VQQHLQQPFS  480
DQQQNLQTQQ QQIYQETFQI ANNQLQQQRS TLATPLCLKP DFTDSSITFP APVTPCMQSM  540
LGSVSSEGSG NVLNFTRVGQ SMRSEQQQQQ AWGQKFPYSQ TNAFTNPVPL PTFPVKGGAV  600
DLDNCSGDPQ NHTLFGVNID SSPLLLPTSV PNLGSSSVEP DVSSMPFGGS GFQNSLYGCM  660
ENSSDLLNSA GQVDPPTRTF VKVYKSGSVG RSLDISRFSS YHELREELGQ MFGIEEQLED  720
PLRSGWQLVF VDRENDVLLL GDDPWESFVN NVWYIKILSP EDVQKLGKQE VDALSSNGDQ  780
RHSNSSYDVR NFTSGISSVG SLEY
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4ldu_A1e-1291631747388Auxin response factor 5
Search in ModeBase
Functional Description ? help Back to Top
Source Description
UniProtAuxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Regulates both stamen and gynoecium maturation. Promotes jasmonic acid production. Partially redundant with ARF6. Involved in fruit initiation. Acts as an inhibitor to stop further carpel development in the absence of fertilization and the generation of signals required to initiate fruit and seed development. {ECO:0000269|PubMed:12036261, ECO:0000269|PubMed:16107481, ECO:0000269|PubMed:16829592}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00033PBMTransfer from AT5G37020Download
Motif logo
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Repressed by miR167. {ECO:0000269|PubMed:17021043}.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_010250595.10.0PREDICTED: auxin response factor 8 isoform X1
RefseqXP_010250602.10.0PREDICTED: auxin response factor 8 isoform X1
RefseqXP_019052664.10.0PREDICTED: auxin response factor 8 isoform X1
SwissprotQ9FGV10.0ARFH_ARATH; Auxin response factor 8
TrEMBLA0A1U7ZAQ30.0A0A1U7ZAQ3_NELNU; Auxin response factor
STRINGXP_010250595.10.0(Nelumbo nucifera)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT5G37020.10.0auxin response factor 8
Publications ? help Back to Top
  1. Liu N, et al.
    Down-regulation of AUXIN RESPONSE FACTORS 6 and 8 by microRNA 167 leads to floral development defects and female sterility in tomato.
    J. Exp. Bot., 2014. 65(9): p. 2507-20
    [PMID:24723401]
  2. Minato N, et al.
    The phytoplasmal virulence factor TENGU causes plant sterility by downregulating of the jasmonic acid and auxin pathways.
    Sci Rep, 2014. 4: p. 7399
    [PMID:25492247]
  3. Ortega-Amaro MA, et al.
    Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance.
    Front Plant Sci, 2014. 5: p. 782
    [PMID:25653657]
  4. Wang Y, et al.
    MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development.
    Plant Physiol., 2015. 168(3): p. 984-99
    [PMID:25941314]
  5. Barik S, et al.
    Coevolution Pattern and Functional Conservation or Divergence of miR167s and their targets across Diverse Plant Species.
    Sci Rep, 2015. 5: p. 14611
    [PMID:26459056]
  6. Pashkovskiy PP,Kartashov AV,Zlobin IE,Pogosyan SI,Kuznetsov VV
    Blue light alters miR167 expression and microRNA-targeted auxin response factor genes in Arabidopsis thaliana plants.
    Plant Physiol. Biochem., 2016. 104: p. 146-54
    [PMID:27031426]
  7. Mlotshwa S,Pruss GJ,MacArthur JL,Reed JW,Vance V
    Developmental Defects Mediated by the P1/HC-Pro Potyviral Silencing Suppressor Are Not Due to Misregulation of AUXIN RESPONSE FACTOR 8.
    Plant Physiol., 2016. 172(3): p. 1853-1861
    [PMID:27688620]
  8. Wójcikowska B,Gaj MD
    Expression profiling of AUXIN RESPONSE FACTOR genes during somatic embryogenesis induction in Arabidopsis.
    Plant Cell Rep., 2017. 36(6): p. 843-858
    [PMID:28255787]
  9. Zhang GZ, et al.
    Ectopic expression of UGT84A2 delayed flowering by indole-3-butyric acid-mediated transcriptional repression of ARF6 and ARF8 genes in Arabidopsis.
    Plant Cell Rep., 2017. 36(12): p. 1995-2006
    [PMID:29027578]
  10. Zheng K, et al.
    Involvement of PACLOBUTRAZOL RESISTANCE6/KIDARI, an Atypical bHLH Transcription Factor, in Auxin Responses in Arabidopsis.
    Front Plant Sci, 2017. 8: p. 1813
    [PMID:29114256]
  11. Liu Z, et al.
    ARF2-ARF4 and ARF5 are Essential for Female and Male Gametophyte Development in Arabidopsis.
    Plant Cell Physiol., 2018. 59(1): p. 179-189
    [PMID:29145642]
  12. Ghelli R, et al.
    A Newly Identified Flower-Specific Splice Variant of AUXIN RESPONSE FACTOR8 Regulates Stamen Elongation and Endothecium Lignification in Arabidopsis.
    Plant Cell, 2018. 30(3): p. 620-637
    [PMID:29514943]