PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Neem_10258_f_1

Organism

Azadirachta indica

Taxonomic ID

124943

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Sapindales; Meliaceae; Azadirachta

Family

WRKY

Protein Properties

Length: 549aa MW: 59884.2 Da PI: 7.563

Description

WRKY family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Neem_10258_f_1	genome	NGD	View Nucleic Acid

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	WRKY	96.6	1.7e-30	298	356	2	59
--SS-EEEEEEE--TT-SS-EEEEEE-ST.T---EEEEEE-SSSTTEEEEEEES--SS- CS WRKY 2 dDgynWrKYGqKevkgsefprsYYrCtsa.gCpvkkkversaedpkvveitYegeHnhe 59 +Dg++WrKYGqK+ kg+++pr+YYrCt+a gCpv+k+v+r+++d +++++tYeg+Hnh+ Neem_10258_f_1 298 SDGCQWRKYGQKMAKGNPCPRAYYRCTMAsGCPVRKQVQRCSQDRTILVTTYEGNHNHP 356 7*************************99**************************7 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:2.20.25.80	1.5E-33	282	358	IPR003657	WRKY domain
SuperFamily	SSF118290	1.83E-27	290	358	IPR003657	WRKY domain
PROSITE profile	PS50811	30.217	292	358	IPR003657	WRKY domain
SMART	SM00774	6.6E-36	297	357	IPR003657	WRKY domain
Pfam	PF03106	2.0E-26	299	356	IPR003657	WRKY domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0006355	Biological Process	regulation of transcription, DNA-templated
GO:0003700	Molecular Function	transcription factor activity, sequence-specific DNA binding
GO:0043565	Molecular Function	sequence-specific DNA binding

Sequence ? help Back to Top
Protein Sequence Length: 549 aa Download sequence Send to blast
MDDRLLGLSI GTDDPSKSCF FHPIFSKIPV NNTTTAACKH DIMGRVDSDR ILINGGDDRV 60 SALQTNISEK RVVNEMNFFA DDVKDKLASD NQETSSVDHV KKESTSTVLD AGEQGIKLDI 120 NTGLNLSTPN SSSEKFNIEK TSKNIEYKPQ ENELAVLQAD LNKMNVENQR LRGLLNQVNS 180 DYRALQMHLL TFMQRQQIRR PGNIPVHEMI NGGKVAVARQ FMDLGQAAAA EKDDQLSHSS 240 SEEARQDQDA AQSAAGWCSN KVPRFSSSRD VENASQTMSM IRKARVSVRA RSEASMISDG 300 CQWRKYGQKM AKGNPCPRAY YRCTMASGCP VRKQVQRCSQ DRTILVTTYE GNHNHPLPPA 360 AMAMASTTSA AVSMLLSGSM PSADGIVNAD FLTKSSLPFS PSLASLSASA PFPTITLDLT 420 HNPIPNPSQF QRQLCQFHFP SPNNLPQNFV TMPQVFAHAV INNQSSFMGL LNSMGLEQPQ 480 SAQNLMPAND TISAATAAAI TADPSFTAAL VAAITSVIGN VHQNQNGNSN NNTTTRNSSD 540 KIEHMKNSL

Sequence ? help Back to Top

Protein Sequence Length: 549 aa Download sequence Send to blast

MDDRLLGLSI GTDDPSKSCF FHPIFSKIPV NNTTTAACKH DIMGRVDSDR ILINGGDDRV  60
SALQTNISEK RVVNEMNFFA DDVKDKLASD NQETSSVDHV KKESTSTVLD AGEQGIKLDI  120
NTGLNLSTPN SSSEKFNIEK TSKNIEYKPQ ENELAVLQAD LNKMNVENQR LRGLLNQVNS  180
DYRALQMHLL TFMQRQQIRR PGNIPVHEMI NGGKVAVARQ FMDLGQAAAA EKDDQLSHSS  240
SEEARQDQDA AQSAAGWCSN KVPRFSSSRD VENASQTMSM IRKARVSVRA RSEASMISDG  300
CQWRKYGQKM AKGNPCPRAY YRCTMASGCP VRKQVQRCSQ DRTILVTTYE GNHNHPLPPA  360
AMAMASTTSA AVSMLLSGSM PSADGIVNAD FLTKSSLPFS PSLASLSASA PFPTITLDLT  420
HNPIPNPSQF QRQLCQFHFP SPNNLPQNFV TMPQVFAHAV INNQSSFMGL LNSMGLEQPQ  480
SAQNLMPAND TISAATAAAI TADPSFTAAL VAAITSVIGN VHQNQNGNSN NNTTTRNSSD  540
KIEHMKNSL

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
2ayd_A	7e-24	284	360	1	76	WRKY transcription factor 1
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Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor involved in the control of processes related to senescence and pathogen defense (PubMed:12000796). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:12000796). Activates the transcription of the SIRK gene and represses its own expression and that of the WRKY42 genes (PubMed:12000796). Modulates phosphate homeostasis and Pi translocation by regulating PHO1 expression (PubMed:25733771). {ECO:0000269\|PubMed:12000796, ECO:0000269\|PubMed:25733771}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: By salicylic acid, ethylene, jasmonic acid, pathogens, wounding and strongly during leaf senescence. {ECO:0000269\|PubMed:11449049, ECO:0000269\|PubMed:11722756}.

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	KF031069	2e-31	KF031069.1 Gossypium hirsutum WRKY transcription factor 1 (WRKY1) mRNA, complete cds.
GenBank	KF669824	2e-31	KF669824.1 Gossypium hirsutum WRKY transcription factor 16 (WRKY16) mRNA, complete cds.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_006452416.2	1e-173	probable WRKY transcription factor 31
Swissprot	Q9C519	1e-106	WRKY6_ARATH; WRKY transcription factor 6
TrEMBL	A0A2H5P286	1e-173	A0A2H5P286_CITUN; Uncharacterized protein
STRING	XP_006475034.1	1e-171	(Citrus sinensis)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM25575	3	3

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G62300.1	1e-75	WRKY family protein

Publications ? help Back to Top
Zhou F, et al. High humidity suppresses ssi4-mediated cell death and disease resistance upstream of MAP kinase activation, H2O2 production and defense gene expression. Plant J., 2004. 39(6): p. 920-32 [PMID:15341634] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P An extracellular subtilase switch for immune priming in Arabidopsis. PLoS Pathog., 2013. 9(6): p. e1003445 [PMID:23818851] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Singh V,Roy S,Singh D,Nandi AK Arabidopsis flowering locus D influences systemic-acquired-resistance- induced expression and histone modifications of WRKY genes. J. Biosci., 2014. 39(1): p. 119-26 [PMID:24499796] Ali MA,Wieczorek K,Kreil DP,Bohlmann H The beet cyst nematode Heterodera schachtii modulates the expression of WRKY transcription factors in syncytia to favour its development in Arabidopsis roots. PLoS ONE, 2014. 9(7): p. e102360 [PMID:25033038] Chai J,Liu J,Zhou J,Xing D Mitogen-activated protein kinase 6 regulates NPR1 gene expression and activation during leaf senescence induced by salicylic acid. J. Exp. Bot., 2014. 65(22): p. 6513-28 [PMID:25210078] Vermeirssen V,De Clercq I,Van Parys T,Van Breusegem F,Van de Peer Y Arabidopsis ensemble reverse-engineered gene regulatory network discloses interconnected transcription factors in oxidative stress. Plant Cell, 2014. 26(12): p. 4656-79 [PMID:25549671] Xiao D, et al. SENESCENCE-SUPPRESSED PROTEIN PHOSPHATASE Directly Interacts with the Cytoplasmic Domain of SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE and Negatively Regulates Leaf Senescence in Arabidopsis. Plant Physiol., 2015. 169(2): p. 1275-91 [PMID:26304848] Bakshi M, et al. WRKY6 restricts Piriformospora indica-stimulated and phosphate-induced root development in Arabidopsis. BMC Plant Biol., 2015. 15: p. 305 [PMID:26718529] Huang Y,Feng CZ,Ye Q,Wu WH,Chen YF Arabidopsis WRKY6 Transcription Factor Acts as a Positive Regulator of Abscisic Acid Signaling during Seed Germination and Early Seedling Development. PLoS Genet., 2016. 12(2): p. e1005833 [PMID:26829043] Ramírez V,López A,Mauch-Mani B,Gil MJ,Vera P Correction: An Extracellular Subtilase Switch for Immune Priming in Arabidopsis. PLoS Pathog., 2016. 12(11): p. e1006003 [PMID:27806116] Li H, et al. BZR1 Positively Regulates Freezing Tolerance via CBF-Dependent and CBF-Independent Pathways in Arabidopsis. Mol Plant, 2017. 10(4): p. 545-559 [PMID:28089951] Stetter MG,Benz M,Ludewig U Increased root hair density by loss of WRKY6 in Arabidopsis thaliana. PeerJ, 2017. 5: p. e2891 [PMID:28149680] Zhou X, et al. StMYB44 negatively regulates phosphate transport by suppressing expression of PHOSPHATE1 in potato. J. Exp. Bot., 2017. 68(5): p. 1265-1281 [PMID:28338870] Bakshi M, et al. Piriformospora indica Reprograms Gene Expression in Arabidopsis Phosphate Metabolism Mutants But Does Not Compensate for Phosphate Limitation. Front Microbiol, 2017. 8: p. 1262 [PMID:28747898] Zhang Y, et al. DELLA proteins negatively regulate dark-induced senescence and chlorophyll degradation in Arabidopsis through interaction with the transcription factor WRKY6. Plant Cell Rep., 2018. 37(7): p. 981-992 [PMID:29574486]