PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Niben101Scf02362g02014.1
Common NameNbWRKY8
Organism
Nicotiana benthamiana
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana
Family WRKY
Protein Properties Length: 538aa    MW: 60167.8 Da    PI: 8.1674
Description WRKY family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Niben101Scf02362g02014.1genomeBTI-
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1WRKY107.47.1e-34212269260
                               --SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS-- CS
                      WRKY   2 dDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhek 60 
                               +DgynWrKYGqK+vkgse+prsYY+Ct+++Cp+kkkvers  d++++ei+Y+g+Hnh+k
  Niben101Scf02362g02014.1 212 EDGYNWRKYGQKQVKGSENPRSYYKCTFPNCPTKKKVERSL-DGQITEIVYKGNHNHPK 269
                               8****************************************.***************85 PP
2WRKY105.92.1e-33379437159
                               ---SS-EEEEEEE--TT-SS-EEEEEE-STT---EEEEEE-SSSTTEEEEEEES--SS- CS
                      WRKY   1 ldDgynWrKYGqKevkgsefprsYYrCtsagCpvkkkversaedpkvveitYegeHnhe 59 
                               ldDgy+WrKYGqK+vkg+++prsYY+Cts+gCpv+k+ver+++d + v++tYeg+Hnh+
  Niben101Scf02362g02014.1 379 LDDGYRWRKYGQKVVKGNPNPRSYYKCTSQGCPVRKHVERASQDIRSVITTYEGKHNHD 437
                               59********************************************************7 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:2.20.25.803.3E-29199270IPR003657WRKY domain
PROSITE profilePS5081123.789206270IPR003657WRKY domain
SuperFamilySSF1182901.44E-25207270IPR003657WRKY domain
SMARTSM007748.9E-37211269IPR003657WRKY domain
PfamPF031061.8E-25212268IPR003657WRKY domain
Gene3DG3DSA:2.20.25.801.5E-37364439IPR003657WRKY domain
SuperFamilySSF1182903.66E-29371439IPR003657WRKY domain
PROSITE profilePS5081138.939374439IPR003657WRKY domain
SMARTSM007748.8E-39379438IPR003657WRKY domain
PfamPF031069.3E-26380437IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0009753Biological Processresponse to jasmonic acid
GO:0009788Biological Processnegative regulation of abscisic acid-activated signaling pathway
GO:0009938Biological Processnegative regulation of gibberellic acid mediated signaling pathway
GO:0045893Biological Processpositive regulation of transcription, DNA-templated
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 538 aa     Download sequence    Send to blast
MAASSTIIDT SSMQNSSFSF TDLLASDDYP TKSKGLGDRI AERTGSGIPK FKSLPPPSLP  60
LSPPPFSPSS YFAIPPGLSP TELLDSPLLL SASNILPSPT TGTFPAQAFN WKSSSNSSHQ  120
GVKQEDKNYS DFSFQPQFGQ SFASVSSSQT NLVPLGKQSW NYQESRKQND QSSENANQRT  180
EFNTIQNLKN NAQSNQYNNQ SSQSIREQKR AEDGYNWRKY GQKQVKGSEN PRSYYKCTFP  240
NCPTKKKVER SLDGQITEIV YKGNHNHPKP QSTRRSSSTA SSLAVQPYNT QINEIPDHQS  300
YGSNATPENS SISFGDDDHD REQSSQKSRS RGDDFDDEEE PDSKRWKRES ESEILSAPGS  360
RTVREPRVVV QTTSDIDILD DGYRWRKYGQ KVVKGNPNPR SYYKCTSQGC PVRKHVERAS  420
QDIRSVITTY EGKHNHDVPA ARGSGINRPV APTITYNNGA NNAMVIRPSV TSQIPLQSIR  480
PQQSPFTLEM LQKPSNYNGF SGYANSEDSY ENQLQDNNVF SRAKDEPRDD MFMETLLC
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1wj2_A5e-33203440878Probable WRKY transcription factor 4
2lex_A5e-33203440878Probable WRKY transcription factor 4
Search in ModeBase
Functional Description ? help Back to Top
Source Description
UniProtTranscription activator (PubMed:26025535). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (PubMed:19199048, PubMed:26025535). Negative regulator of both gibberellic acid (GA) and abscisic acid (ABA) signaling in aleurone cells, probably by interfering with GAM1, via the specific repression of GA- and ABA-induced promoters (PubMed:15618416, PubMed:19199048, PubMed:26025535). {ECO:0000269|PubMed:15618416, ECO:0000269|PubMed:19199048, ECO:0000269|PubMed:26025535}.
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Induced by abscisic acid (ABA) in aleurone cells, embryos, roots and leaves (PubMed:15618416, PubMed:19199048). Slightly down-regulated by gibberellic acid (GA) (PubMed:15618416). Accumulates in response to jasmonic acid (MeJA) (By similarity). {ECO:0000250|UniProtKB:Q6B6R4, ECO:0000269|PubMed:15618416, ECO:0000269|PubMed:19199048}.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_009790461.10.0PREDICTED: probable WRKY transcription factor 26
RefseqXP_016492389.10.0PREDICTED: probable WRKY transcription factor 33
SwissprotQ6IEQ71e-133WRK24_ORYSJ; WRKY transcription factor WRKY24
TrEMBLD2KU330.0D2KU33_NICBE; Double WRKY type transfactor
STRINGXP_009790461.10.0(Nicotiana sylvestris)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
AsteridsOGEA22862460
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT2G38470.11e-115WRKY DNA-binding protein 33
Publications ? help Back to Top
  1. Kikuchi S, et al.
    Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice.
    Science, 2003. 301(5631): p. 376-9
    [PMID:12869764]
  2. Zhang ZL, et al.
    A negative regulator encoded by a rice WRKY gene represses both abscisic acid and gibberellins signaling in aleurone cells.
    Plant Mol. Biol., 2009. 70(1-2): p. 139-51
    [PMID:19199048]
  3. Ishihama N,Yamada R,Yoshioka M,Katou S,Yoshioka H
    Phosphorylation of the Nicotiana benthamiana WRKY8 transcription factor by MAPK functions in the defense response.
    Plant Cell, 2011. 23(3): p. 1153-70
    [PMID:21386030]
  4. Basu S,Roychoudhury A
    Expression profiling of abiotic stress-inducible genes in response to multiple stresses in rice (Oryza sativa L.) varieties with contrasting level of stress tolerance.
    Biomed Res Int, 2014. 2014: p. 706890
    [PMID:25110688]
  5. Zhang L, et al.
    Three WRKY transcription factors additively repress abscisic acid and gibberellin signaling in aleurone cells.
    Plant Sci., 2015. 236: p. 214-22
    [PMID:26025535]