PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

OPUNC05G25080.1

Organism

Oryza punctata

Taxonomic ID

4537

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae; Oryzinae; Oryza

Family

bHLH

Protein Properties

Length: 412aa MW: 44105.4 Da PI: 4.7213

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
OPUNC05G25080.1	genome	OGE	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	40.8	4e-13	175	226	1	55
CHHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 1 rrrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +r++h ++Er+RR+++N+ + Lr+l+P + + ++ + a+i A+e+I++L+ OPUNC05G25080.1 175 QRMTHIAVERNRRRQMNEYLRVLRSLMPGS---YVQRGDQASIIGGAIEFIRELE 226 79**************************...9****************996 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Gene3D	G3DSA:4.10.280.10	1.2E-13	173	229	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	1.20E-13	173	230	No hit	No description
SuperFamily	SSF47459	6.41E-17	174	243	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS50888	15.897	174	225	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	1.7E-10	175	226	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	1.0E-9	180	231	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd04873	0.00294	338	396	No hit	No description
PROSITE profile	PS51671	10.646	338	412	IPR002912	ACT domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0010377	Biological Process	guard cell fate commitment
GO:0045597	Biological Process	positive regulation of cell differentiation
GO:0045893	Biological Process	positive regulation of transcription, DNA-templated
GO:0051782	Biological Process	negative regulation of cell division
GO:0005634	Cellular Component	nucleus
GO:0016597	Molecular Function	amino acid binding
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 412 aa Download sequence Send to blast
MDKERRSHMD SFAPLDEAPP EETAVTGGEM VDYMLGQPPP TTPGQQSQVS FDKLTFSDVL 60 QFADFGPKLA LNQPAASENG GGGGDDDDDD SYFLRFQSLP SLPIVPPPRG GAHQGDEQEG 120 SKQTVDAGGV SESTTLVQQA DGFGGGRAEK AGDQGKSGRR KRPRTVKTSE EVESQRMTHI 180 AVERNRRRQM NEYLRVLRSL MPGSYVQRGD QASIIGGAIE FIRELEQLIQ CLESQKRRRL 240 YGGDAPAPPR PVADGAVLPA PMQQQQPPAT PFFPPTIPFP ASSGGGSGDG GAGVAKILDL 300 DASGGGGGDV AGGVREEMAE NKSCIADIEV RVLGVDAMIK ILSRRRPGQL IKTVAALEEM 360 QMSILHTNIT TIDQTVLYSF NVKIAGEARF SAEDIAGAVH QILSFIDVNY TL

Sequence ? help Back to Top

Protein Sequence Length: 412 aa Download sequence Send to blast

MDKERRSHMD SFAPLDEAPP EETAVTGGEM VDYMLGQPPP TTPGQQSQVS FDKLTFSDVL  60
QFADFGPKLA LNQPAASENG GGGGDDDDDD SYFLRFQSLP SLPIVPPPRG GAHQGDEQEG  120
SKQTVDAGGV SESTTLVQQA DGFGGGRAEK AGDQGKSGRR KRPRTVKTSE EVESQRMTHI  180
AVERNRRRQM NEYLRVLRSL MPGSYVQRGD QASIIGGAIE FIRELEQLIQ CLESQKRRRL  240
YGGDAPAPPR PVADGAVLPA PMQQQQPPAT PFFPPTIPFP ASSGGGSGDG GAGVAKILDL  300
DASGGGGGDV AGGVREEMAE NKSCIADIEV RVLGVDAMIK ILSRRRPGQL IKTVAALEEM  360
QMSILHTNIT TIDQTVLYSF NVKIAGEARF SAEDIAGAVH QILSFIDVNY TL

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	175	186	RMTHIAVERNRR
2	235	239	KRRRL

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator (PubMed:17088607, PubMed:17183265, PubMed:17183267). Together with MYB88 and MYB124, ensures that stomata contain just two guard cells (GCs) by enforcing a single symmetric precursor cell division before stomatal maturity (PubMed:24571519). Together with SPCH and MUTE, regulates the stomata formation. Required to promote differentiation and morphogenesis of stomatal guard cells and to halt proliferative divisions in their immediate precursors. Mediates the formation of stomata (PubMed:17088607, PubMed:17183265, PubMed:17183267). Prevents histone H3K27me3 marks and derepresses stem cell gene expression (PubMed:24654956). {ECO:0000269\|PubMed:17088607, ECO:0000269\|PubMed:17183265, ECO:0000269\|PubMed:17183267, ECO:0000269\|PubMed:24571519, ECO:0000269\|PubMed:24654956}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Inhibited by low relative humidity (LRH) via epigenetic CG methylation, thus leading to a reduced stomatal index. {ECO:0000269\|PubMed:22442411}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AC137608	0.0	AC137608.2 Oryza sativa Japonica Group cultivar Nipponbare chromosome 5 clone OSJNBa0009C07, complete sequence.
GenBank	AP014961	0.0	AP014961.1 Oryza sativa Japonica Group DNA, chromosome 5, cultivar: Nipponbare, complete sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_015638786.1	0.0	transcription factor FAMA
Swissprot	Q56YJ8	1e-108	FAMA_ARATH; Transcription factor FAMA
TrEMBL	A0A0E0L6C0	0.0	A0A0E0L6C0_ORYPU; Uncharacterized protein
STRING	OPUNC05G25080.1	0.0	(Oryza punctata)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Monocots	OGMP9502	33	40

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G24140.1	1e-55	bHLH family protein

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Matos JL, et al. Irreversible fate commitment in the Arabidopsis stomatal lineage requires a FAMA and RETINOBLASTOMA-RELATED module. Elife, 2015. [PMID:25303364] Chen L, et al. NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis. Development, 2016. 143(9): p. 1600-11 [PMID:26989174] Lee JH,Jung JH,Park CM Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis. Plant Cell, 2017. 29(11): p. 2817-2830 [PMID:29070509] Han X, et al. Jasmonate Negatively Regulates Stomatal Development in Arabidopsis Cotyledons. Plant Physiol., 2018. 176(4): p. 2871-2885 [PMID:29496884] Li X,Yang R,Chen H The Arabidopsis thaliana Mediator subunit MED8 regulates plant immunity to Botrytis Cinerea through interacting with the basic helix-loop-helix (bHLH) transcription factor FAMA. PLoS ONE, 2018. 13(3): p. e0193458 [PMID:29513733]