PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

PSME_00035816-RA

Organism

Pseudotsuga menziesii

Taxonomic ID

3357

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Acrogymnospermae; Pinidae; Pinales; Pinaceae; Pseudotsuga

Family

bHLH

Protein Properties

Length: 338aa MW: 37597.9 Da PI: 6.9457

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
PSME_00035816-RA	genome	PRS	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	42.1	1.6e-13	142	193	1	55
CHHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 1 rrrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +r++h e+Er+RR+++N ++ Lr+l+P++ + ++ + a+i A++ +k+L+ PSME_00035816-RA 142 QRMTHIEVERNRRKQMNAHLDALRSLIPQS---YIQRGDQASIIGGAIDLVKELE 193 79**************************...9***************9995 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF47459	7.59E-16	134	202	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	2.1E-13	141	196	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS50888	15.396	141	192	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	1.84E-12	141	197	No hit	No description
Pfam	PF00010	1.0E-10	142	193	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	2.4E-10	147	198	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS51671	8.52	266	338	IPR002912	ACT domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0008152	Biological Process	metabolic process
GO:0016597	Molecular Function	amino acid binding
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 338 aa Download sequence Send to blast
MEIMEEMIEI PRLSDNLGLN IPWLHGGPFR KRLTSSPLHS EPSGKQGNIR PNLDNYFTRL 60 VQGLEPLESC VTDLSELKSA GNIDTFNAVH SLSDTAEDGI SDTTQENQQN VISLAVPAPD 120 AGKGKKRKRL KVSKNSEEVQ SQRMTHIEVE RNRRKQMNAH LDALRSLIPQ SYIQRGDQAS 180 IIGGAIDLVK ELEQVLQSLQ FQKIKMENVD RTDHPKNCLS ILPLHQSSAR CNSKCVLDTS 240 SSQLRAAEGK SSIADIEVTL IETHCSLKIL SKKKHGQLLK IVTALEGFHM TILHLNITSM 300 DEAVLFSFNV KVEDECPLTS ANDIANSLQQ ILRIVHAC

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	142	153	RMTHIEVERNRR

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator (PubMed:17088607, PubMed:17183265, PubMed:17183267). Together with MYB88 and MYB124, ensures that stomata contain just two guard cells (GCs) by enforcing a single symmetric precursor cell division before stomatal maturity (PubMed:24571519). Together with SPCH and MUTE, regulates the stomata formation. Required to promote differentiation and morphogenesis of stomatal guard cells and to halt proliferative divisions in their immediate precursors. Mediates the formation of stomata (PubMed:17088607, PubMed:17183265, PubMed:17183267). Prevents histone H3K27me3 marks and derepresses stem cell gene expression (PubMed:24654956). {ECO:0000269\|PubMed:17088607, ECO:0000269\|PubMed:17183265, ECO:0000269\|PubMed:17183267, ECO:0000269\|PubMed:24571519, ECO:0000269\|PubMed:24654956}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Inhibited by low relative humidity (LRH) via epigenetic CG methylation, thus leading to a reduced stomatal index. {ECO:0000269\|PubMed:22442411}.

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	BT117675	0.0	BT117675.1 Picea glauca clone GQ03907_D16 mRNA sequence.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_010266731.1	4e-66	PREDICTED: transcription factor bHLH94-like
Swissprot	Q56YJ8	4e-59	FAMA_ARATH; Transcription factor FAMA
TrEMBL	B8LLL6	2e-75	B8LLL6_PICSI; Uncharacterized protein
STRING	XP_010266731.1	1e-65	(Nelumbo nucifera)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G24140.1	2e-58	bHLH family protein

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Matos JL, et al. Irreversible fate commitment in the Arabidopsis stomatal lineage requires a FAMA and RETINOBLASTOMA-RELATED module. Elife, 2015. [PMID:25303364] Chen L, et al. NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis. Development, 2016. 143(9): p. 1600-11 [PMID:26989174] Lee JH,Jung JH,Park CM Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis. Plant Cell, 2017. 29(11): p. 2817-2830 [PMID:29070509] Han X, et al. Jasmonate Negatively Regulates Stomatal Development in Arabidopsis Cotyledons. Plant Physiol., 2018. 176(4): p. 2871-2885 [PMID:29496884] Li X,Yang R,Chen H The Arabidopsis thaliana Mediator subunit MED8 regulates plant immunity to Botrytis Cinerea through interacting with the basic helix-loop-helix (bHLH) transcription factor FAMA. PLoS ONE, 2018. 13(3): p. e0193458 [PMID:29513733]