PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Pbr010447.1

Organism

Pyrus bretschneideri

Taxonomic ID

225117

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae; Maloideae; Maleae; Pyrus

Family

EIL

Protein Properties

Length: 811aa MW: 90918.6 Da PI: 6.9779

Description

EIL family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Pbr010447.1	genome	CPETR	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	EIN3	201.1	9e-62	42	172	2	138
XXXXXXXXXXXXXXXXXXXXXX..XXXXX.XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX CS EIN3 2 elkkrmwkdqmllkrlkerkkqlledkeaatgakksnksneqarrkkmsraQDgiLkYMlkemevcnaqGfvYgiipekgkpvegasdsLraWWkekv 99 el++rmw+d+mllkrl+e++k ke+ ++++++++s+eqarrkkmsraQDgiLkYMlk+mevc+aqGfvYgiipekgkpv+gasd+LraWWkekv Pbr010447.1 42 ELERRMWRDRMLLKRLREQTKG----KEK-VDNARQRQSQEQARRKKMSRAQDGILKYMLKMMEVCKAQGFVYGIIPEKGKPVSGASDNLRAWWKEKV 134 99**************9998....676.89999*********************************************************** PP XXXXXXXXXXXXXXXXXXXXXXXXXX....XX----STT CS EIN3 100 efdrngpaaiskyqaknlilsgesslqtersseshslse 138 +fdrngpaaiskyqa++ i+++++++++ s++h+l+e Pbr010447.1 135 RFDRNGPAAISKYQADHSIPGKNEDCSA-VVSTPHTLQE 172 **********************77.9*****98 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF04873	1.3E-57	42	172	No hit	No description
SuperFamily	SSF56112	1.13E-75	284	340	IPR011009	Protein kinase-like domain
Gene3D	G3DSA:3.30.200.20	2.5E-24	287	430	No hit	No description
SMART	SM00220	1.8E-87	296	592	IPR000719	Protein kinase domain
PROSITE profile	PS50011	47.099	296	592	IPR000719	Protein kinase domain
CDD	cd05117	1.06E-110	296	591	No hit	No description
PROSITE pattern	PS00107	0	302	330	IPR017441	Protein kinase, ATP binding site
Pfam	PF00069	2.7E-57	350	592	IPR000719	Protein kinase domain
SuperFamily	SSF56112	1.13E-75	369	606	IPR011009	Protein kinase-like domain
Gene3D	G3DSA:1.10.510.10	1.1E-58	431	605	No hit	No description
PROSITE pattern	PS00108	0	453	465	IPR008271	Serine/threonine-protein kinase, active site
SuperFamily	SSF47473	1.94E-33	637	801	IPR011992	EF-hand domain pair
Gene3D	G3DSA:1.10.238.10	1.9E-37	644	801	IPR011992	EF-hand domain pair
PROSITE profile	PS50222	9.939	686	721	IPR002048	EF-hand domain
SMART	SM00054	1.2	690	718	IPR002048	EF-hand domain
CDD	cd00051	1.22E-7	693	752	No hit	No description
Pfam	PF13202	0.012	694	710	IPR002048	EF-hand domain
PRINTS	PR00450	4.0E-7	694	715	No hit	No description
PROSITE pattern	PS00018	0	699	711	IPR018247	EF-Hand 1, calcium-binding site
PRINTS	PR00450	4.0E-7	718	737	No hit	No description
PROSITE profile	PS50222	13.119	722	757	IPR002048	EF-hand domain
Pfam	PF13499	2.7E-14	726	794	IPR002048	EF-hand domain
SMART	SM00054	6.2E-4	726	754	IPR002048	EF-hand domain
CDD	cd00051	2.21E-12	727	794	No hit	No description
PROSITE pattern	PS00018	0	735	747	IPR018247	EF-Hand 1, calcium-binding site
PROSITE profile	PS50222	14.179	764	799	IPR002048	EF-hand domain
SMART	SM00054	2.4E-6	768	796	IPR002048	EF-hand domain
PROSITE pattern	PS00018	0	777	789	IPR018247	EF-Hand 1, calcium-binding site
PRINTS	PR00450	4.0E-7	784	804	No hit	No description

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0009608	Biological Process	response to symbiont
GO:0009877	Biological Process	nodulation
GO:0046777	Biological Process	protein autophosphorylation
GO:0004683	Molecular Function	calmodulin-dependent protein kinase activity
GO:0005509	Molecular Function	calcium ion binding
GO:0005524	Molecular Function	ATP binding
GO:0009931	Molecular Function	calcium-dependent protein serine/threonine kinase activity

Sequence ? help Back to Top
Protein Sequence Length: 811 aa Download sequence Send to blast
MGFCGNLDFL TAPSGEGDAA PEHEPEAAVE EDYSDEEMDV GELERRMWRD RMLLKRLREQ 60 TKGKEKVDNA RQRQSQEQAR RKKMSRAQDG ILKYMLKMME VCKAQGFVYG IIPEKGKPVS 120 GASDNLRAWW KEKVRFDRNG PAAISKYQAD HSIPGKNEDC SAVVSTPHTL QESSSSRIRI 180 ENKQERKQSV TAAAPTTNHH PSPQAAKASA SNAVPFCFVD FITILPLPDS RHLQTKPRDR 240 NDYLLDLWLD RLPRFHFFPL GSVSAFPVRF HQGLFDFVFI GIVTMGQETR RLADEYEISE 300 ILGRGGFSVV RKGISRKSSS SSDKTDVAIK TLKRPFAPSN PPPLPPHARR NDQNSIAAAA 360 FQTRKQVSIS NVLLTNEILV MRKIVENVSP HPNVIDLYDV YEDENGVHLV LELCSGGELF 420 DRIVKQERYS EAGAAAVVRQ IAKGLAALHR SNIVHRDLKP ENCLFLDKTV DSPLKIMDFG 480 LSSVEEFTDP VVGLFGSIDY VSPEALSQGQ VTSKSDMWAL GVILYILLSG HPPFIAQSNR 540 QKQQMIMAGE FSFYEKTWKG ISLSAKQLIS DLLKVDPDKR PSAQELLDHP WVVGLSARED 600 QMDTEIVSRL QSFNARRKLR AAAIASVWTS SIFLRTKKLK SLLGSYDLKP DEIKNLSSHF 660 KKICVKGDNA TLSEFEEVLK AMNMSSLIPL APRIFDLFDN NRDGTIDMRE ILCGFSNLKN 720 SQGDDALRLC FQMYDTDRSG CISKEEVASM LRALPDDCLP ADITEPGKLD EIFDRMDANS 780 DGKVTFDEFK AAMQRDSSLQ DVVLSHLRPI *

Sequence ? help Back to Top

Protein Sequence Length: 811 aa Download sequence Send to blast

MGFCGNLDFL TAPSGEGDAA PEHEPEAAVE EDYSDEEMDV GELERRMWRD RMLLKRLREQ  60
TKGKEKVDNA RQRQSQEQAR RKKMSRAQDG ILKYMLKMME VCKAQGFVYG IIPEKGKPVS  120
GASDNLRAWW KEKVRFDRNG PAAISKYQAD HSIPGKNEDC SAVVSTPHTL QESSSSRIRI  180
ENKQERKQSV TAAAPTTNHH PSPQAAKASA SNAVPFCFVD FITILPLPDS RHLQTKPRDR  240
NDYLLDLWLD RLPRFHFFPL GSVSAFPVRF HQGLFDFVFI GIVTMGQETR RLADEYEISE  300
ILGRGGFSVV RKGISRKSSS SSDKTDVAIK TLKRPFAPSN PPPLPPHARR NDQNSIAAAA  360
FQTRKQVSIS NVLLTNEILV MRKIVENVSP HPNVIDLYDV YEDENGVHLV LELCSGGELF  420
DRIVKQERYS EAGAAAVVRQ IAKGLAALHR SNIVHRDLKP ENCLFLDKTV DSPLKIMDFG  480
LSSVEEFTDP VVGLFGSIDY VSPEALSQGQ VTSKSDMWAL GVILYILLSG HPPFIAQSNR  540
QKQQMIMAGE FSFYEKTWKG ISLSAKQLIS DLLKVDPDKR PSAQELLDHP WVVGLSARED  600
QMDTEIVSRL QSFNARRKLR AAAIASVWTS SIFLRTKKLK SLLGSYDLKP DEIKNLSSHF  660
KKICVKGDNA TLSEFEEVLK AMNMSSLIPL APRIFDLFDN NRDGTIDMRE ILCGFSNLKN  720
SQGDDALRLC FQMYDTDRSG CISKEEVASM LRALPDDCLP ADITEPGKLD EIFDRMDANS  780
DGKVTFDEFK AAMQRDSSLQ DVVLSHLRPI *

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
4mvf_A	5e-67	291	795	54	498	Calcium-dependent protein kinase 2
Search in ModeBase

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	615	639	RRKLRAAAIASVWTSSIFLRTKKLK

Functional Description ? help Back to Top
Source	Description
UniProt	During nodulation, plays a central role in bacterial infection and contributes to nodule organogenesis (PubMed:22874912). Protein kinase that recognizes the calcium spiking induced by Nod factors and translates this signal to components controlling nodulation and mycorrhizal infection responses. May phosphorylate the NSP1 protein (PubMed:14963335, PubMed:15070781). Required in epidermal and cortical cells to promote infection thread (IT) formation in root hairs (PubMed:22874912). {ECO:0000269\|PubMed:14963335, ECO:0000269\|PubMed:15070781, ECO:0000269\|PubMed:22874912}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Pbr010447.1

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Small up-regulation in nodules. {ECO:0000269\|PubMed:14963335}.

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	Z17313	0.0	Z17313.1 Malus domestica mRNA for calcium/calmodulin-dependent serine/threonine protein kinase.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_009339247.1	0.0	PREDICTED: calcium/calmodulin-dependent serine/threonine-protein kinase
Refseq	XP_009339248.1	0.0	PREDICTED: calcium/calmodulin-dependent serine/threonine-protein kinase
Swissprot	Q6RET7	0.0	CCAMK_MEDTR; Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase DMI-3
TrEMBL	A0A498JH27	0.0	A0A498JH27_MALDO; Uncharacterized protein
STRING	XP_009339247.1	0.0	(Pyrus x bretschneideri)
STRING	XP_009339248.1	0.0	(Pyrus x bretschneideri)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Fabids	OGEF8826	3	4

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G20770.1	4e-64	EIL family protein

Publications ? help Back to Top
Lévy J, et al. A putative Ca2+ and calmodulin-dependent protein kinase required for bacterial and fungal symbioses. Science, 2004. 303(5662): p. 1361-4 [PMID:14963335] Mitra RM, et al. A Ca2+/calmodulin-dependent protein kinase required for symbiotic nodule development: Gene identification by transcript-based cloning. Proc. Natl. Acad. Sci. U.S.A., 2004. 101(13): p. 4701-5 [PMID:15070781] Messinese E, et al. A novel nuclear protein interacts with the symbiotic DMI3 calcium- and calmodulin-dependent protein kinase of Medicago truncatula. Mol. Plant Microbe Interact., 2007. 20(8): p. 912-21 [PMID:17722695] Rival P, et al. Epidermal and cortical roles of NFP and DMI3 in coordinating early steps of nodulation in Medicago truncatula. Development, 2012. 139(18): p. 3383-91 [PMID:22874912] Rival P,Bono JJ,Gough C,Bensmihen S,Rosenberg C Cell autonomous and non-cell autonomous control of rhizobial and mycorrhizal infection in Medicago truncatula. Plant Signal Behav, 2013. 8(2): p. e22999 [PMID:23221781] Tang H, et al. An improved genome release (version Mt4.0) for the model legume Medicago truncatula. BMC Genomics, 2014. 15: p. 312 [PMID:24767513]