PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Pgl006469

Organism

Picea glauca

Taxonomic ID

3330

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Acrogymnospermae; Pinidae; Pinales; Pinaceae; Picea

Family

bHLH

Protein Properties

Length: 239aa MW: 26584.1 Da PI: 6.397

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
gnl\|UG\|Pgl#S55347473	PU_unref	Unigene	View CDS
PUT-175a-Picea_glauca-16980	PU_ref	plantGDB	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	24.4	5.3e-08	47	94	4	55
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +h+ +Er RR++i ++ L++l+P + +k a +L + ++Y++sLq Pgl006469 47 SHSLAERVRREKISARMKYLQDLVPGC----NKVTGRAVMLDEIINYVQSLQ 94 8***********************9....67799*************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
SuperFamily	SSF47459	3.14E-16	41	110	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	2.20E-9	41	98	No hit	No description
Gene3D	G3DSA:4.10.280.10	2.3E-16	43	110	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS50888	14.672	43	93	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	4.0E-5	47	94	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	1.6E-8	49	99	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 239 aa Download sequence Send to blast
MAKTEQSNSD NSVDSSPKPA RANPKPLENQ KQDYIHVRAR RGQATDSHSL AERVRREKIS 60 ARMKYLQDLV PGCNKVTGRA VMLDEIINYV QSLQRQVEFL SMKLAAVNPS LEFNIDNFFA 120 REMMMPVPPP GNFSSLEGMS PNLAPYIHFQ QMLQQQASLQ AGACSGLDVP TLDTTPSLHR 180 PGSAEMFGDA ANFQGHAPST WDSELQNVFS MGLVQDRQPP FSSEGLPGHL EPNHMKMEM

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, and stems. {ECO:0000269\|PubMed:12679534}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription factor that binds DNA to G box 5'-CACGTG-3' and, to a lower extent, to E-box 5'-CANNTG-3' in vitro. Binds to chromatin DNA of the FT gene and promotes its expression, and thus triggers flowering in response to blue light. {ECO:0000269\|PubMed:18988809, ECO:0000269\|PubMed:24130508}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Accumulates strongly in response to blue light due to reduced preventing 26S proteasome-mediated degradation in an ADO1/ZTL and ADO2/LKP2 dependent manner, but levels decrease in the absence of blue light via 26S proteasome degradation (at protein level). {ECO:0000269\|PubMed:24101505}.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_024030235.1	4e-73	transcription factor bHLH63 isoform X1
Refseq	XP_024030241.1	4e-73	transcription factor bHLH63 isoform X2
Swissprot	Q8GY61	5e-52	BH063_ARATH; Transcription factor bHLH63
TrEMBL	A0A2R6Q5T8	2e-71	A0A2R6Q5T8_ACTCH; Transcription factor bHLH63 like
STRING	XP_008236908.1	6e-72	(Prunus mume)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G34530.1	3e-52	cryptochrome-interacting basic-helix-loop-helix 1

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Yang D,Zhao W,Meng Y,Li H,Liu B A CIB1-LIKE transcription factor GmCIL10 from soybean positively regulates plant flowering. Sci China Life Sci, 2015. 58(3): p. 261-9 [PMID:25651969] Katsura Y, et al. An optogenetic system for interrogating the temporal dynamics of Akt. Sci Rep, 2015. 5: p. 14589 [PMID:26423353] Taslimi A, et al. Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. Nat. Chem. Biol., 2016. 12(6): p. 425-30 [PMID:27065233] Liu Q, et al. The Blue Light-Dependent Polyubiquitination and Degradation of Arabidopsis Cryptochrome2 Requires Multiple E3 Ubiquitin Ligases. Plant Cell Physiol., 2016. 57(10): p. 2175-2186 [PMID:27516416] Pathak GP, et al. Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res., 2017. 45(20): p. e167 [PMID:28431041] Duan L, et al. Understanding CRY2 interactions for optical control of intracellular signaling. Nat Commun, 2017. 8(1): p. 547 [PMID:28916751] Quejada JR, et al. Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1. Nucleic Acids Res., 2017. 45(20): p. e172 [PMID:29040770] Liu Y, et al. CIB1 and CO interact to mediate CRY2-dependent regulation of flowering. EMBO Rep., 2019. [PMID:30126927]