PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Potri.009G136300.2
Common NamePOPTR_0009s13860g
Organism
Populus trichocarpa
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Salicaceae; Saliceae; Populus
Family bHLH
Protein Properties Length: 575aa    MW: 64657.9 Da    PI: 4.8052
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Potri.009G136300.2genomeJGIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH37.64e-12326369755
                         HHHHHHHHHHHHHHHHHHCTSCC.C...TTS-STCHHHHHHHHHHHHHHH CS
                 HLH   7 erErrRRdriNsafeeLrellPk.askapskKlsKaeiLekAveYIksLq 55 
                          +Er+RR+++N+++  Lr+l+P+ +      Kl+ a+iL  A+e++k+Lq
  Potri.009G136300.2 326 VAERKRRKKLNDRLYALRSLVPNiS------KLDRASILGDAIEFVKELQ 369
                         68*********************66......******************9 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
PfamPF142156.9E-197154IPR025610Transcription factor MYC/MYB N-terminal
PROSITE profilePS5088815.955319368IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000835.97E-13321371No hitNo description
SuperFamilySSF474591.57E-16322382IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003532.1E-15325374IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.102.7E-16326382IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000101.0E-9326369IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd048730.00761463525No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0008152Biological Processmetabolic process
GO:0009555Biological Processpollen development
GO:0048657Biological Processanther wall tapetum cell differentiation
GO:0005634Cellular Componentnucleus
GO:0000978Molecular FunctionRNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0016597Molecular Functionamino acid binding
GO:0046983Molecular Functionprotein dimerization activity
Sequence ? help Back to Top
Protein Sequence    Length: 575 aa     Download sequence    Send to blast
MNVVQNLMER LRPLVGVKGW DYCVLWKLSD DRRYIELMDC CCAGTEATQN GEELQFPVSA  60
VLPCRDVMFQ HPGTKSCELL AQLPSSMPLN SGFHAQTLSS NLPRWLNFSS SSDSNVLEET  120
VGTRALIPVP GGLMELFIAK QVPEDQHVID VVTSQCNFLM EQEAMINSTN MDSSLSIDVN  180
VMSENQSKPF LANENEQEDH HSLNIPYDTS LDRLHMSSSP MNNFMHQFNY STDETKTKGD  240
LFQGVESGLQ DMDDLQKSMM ANAESTQMQY MESGLTTKDQ HGNDKESIKL ENGPSAEYSH  300
SDCNDDEDDA KYRRRTGKGP QSKNLVAERK RRKKLNDRLY ALRSLVPNIS KLDRASILGD  360
AIEFVKELQK EAKELQDELE ENSEDEGAKN GNNNNMPPEI LNQNGVNLGA YRSDYAVNGF  420
HVEASGISTV SKQNQDSENS HDKGHQMEAQ VEVAQIDGNE FFVKVFCEHK PGGFVRLMEA  480
LDSLGLEVTN ANVTSNRGLV SNVLKVEKDS EMVQADYVRD SLLELTRDPP RAWPEMPKAS  540
EICCSGMDYP HHDHHQHHLQ NGHMNYNHHH LHHL*
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
5gnj_A9e-133273691254Transcription factor MYC2
5gnj_B9e-133273691254Transcription factor MYC2
5gnj_E9e-133273691254Transcription factor MYC2
5gnj_F9e-133273691254Transcription factor MYC2
5gnj_G9e-133273691254Transcription factor MYC2
5gnj_I9e-133273691254Transcription factor MYC2
5gnj_M9e-133273691254Transcription factor MYC2
5gnj_N9e-133273691254Transcription factor MYC2
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1312333RRRTGKGPQSKNLVAERKRRKK
2327334ERKRRKKL
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
Pth.161960.0bud| catkin
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: First observed a bud stage of 0.6-mm, shortly after microspore release from the postmeiotic tetrads. Later restricted within the tapetum, microspores and anther locule. Still visible within the haploid nuclei, each of which had migrated to the pollen cell wall prior to pollen mitosis I. Not expressed later. {ECO:0000269|PubMed:12535353}.
UniprotTISSUE SPECIFICITY: Mostly expressed in closed, post-meiotic buds, and, to a lower extent, in pre-meiotic buds. Detected in leaves, stems, and flowers. {ECO:0000269|PubMed:12535353, ECO:0000269|PubMed:12679534}.
Functional Description ? help Back to Top
Source Description
UniProtTranscription factor. Plays a crucial role in tapetum development. Required for male fertility and pollen differentiation, especially during the post-meiotic transcriptional regulation of microspore development within the developing anther (PubMed:12535353). Binds E-box regions in the AHL16/TEK promoter. {ECO:0000269|PubMed:12535353, ECO:0000269|PubMed:24804694}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapPotri.009G136300.2
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_006379291.20.0transcription factor ABORTED MICROSPORES isoform X2
SwissprotQ9ZVX21e-154AMS_ARATH; Transcription factor ABORTED MICROSPORES
TrEMBLA0A3N7GZQ80.0A0A3N7GZQ8_POPTR; Uncharacterized protein
STRINGPOPTR_0009s13860.10.0(Populus trichocarpa)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT2G16910.11e-156bHLH family protein
Publications ? help Back to Top
  1. Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH
    Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature.
    Differentiation, 2010. 80(1): p. 1-8
    [PMID:20219281]
  2. Jiang J,Zhang Z,Cao J
    Pollen wall development: the associated enzymes and metabolic pathways.
    Plant Biol (Stuttg), 2013. 15(2): p. 249-63
    [PMID:23252839]
  3. Xu J, et al.
    ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis.
    Plant Cell, 2014. 26(4): p. 1544-1556
    [PMID:24781116]
  4. Qian H, et al.
    Trace concentrations of imazethapyr (IM) affect floral organs development and reproduction in Arabidopsis thaliana: IM-induced inhibition of key genes regulating anther and pollen biosynthesis.
    Ecotoxicology, 2015. 24(1): p. 163-71
    [PMID:25348600]
  5. Xiong SX, et al.
    The transcription factors MS188 and AMS form a complex to activate the expression of CYP703A2 for sporopollenin biosynthesis in Arabidopsis thaliana.
    Plant J., 2016. 88(6): p. 936-946
    [PMID:27460657]
  6. Ferguson AC, et al.
    Biphasic regulation of the transcription factor ABORTED MICROSPORES (AMS) is essential for tapetum and pollen development in Arabidopsis.
    New Phytol., 2017. 213(2): p. 778-790
    [PMID:27787905]
  7. Lou Y, et al.
    Positive regulation of AMS by TDF1 and the formation of a TDF1-AMS complex are required for anther development in Arabidopsis thaliana.
    New Phytol., 2018. 217(1): p. 378-391
    [PMID:28940573]
  8. Bi H,Fan W,Zhang P
    C4 Protein of Sweet Potato Leaf Curl Virus Regulates Brassinosteroid Signaling Pathway through Interaction with AtBIN2 and Affects Male Fertility in Arabidopsis.
    Front Plant Sci, 2017. 8: p. 1689
    [PMID:29021807]