PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Pp3c20_590V3.2.p

Common Name

PHYPADRAFT_97743

Organism

Physcomitrella patens

Taxonomic ID

3218

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Bryophyta; Bryophytina; Bryopsida; Funariidae; Funariales; Funariaceae; Physcomitrella

Family

bHLH

Protein Properties

Length: 155aa MW: 17415.7 Da PI: 6.5002

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Pp3c20_590V3.2.p	genome	COSMOSS	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	15.8	2.6e-05	25	68	8	55
HHHHHHHHHHHHHHHHHCTSCCC...TTS-.STCHHHHHHHHHHHHHHH CS HLH 8 rErrRRdriNsafeeLrellPkaskapskK.lsKaeiLekAveYIksLq 55 +E R ++i++++ L++l+P + +K K ++L + ++Y++sLq Pp3c20_590V3.2.p 25 AEWVRCEKIRKRMKYLQDLVPGC-----RKvTGKTDMLDEIINYVQSLQ 68 5667999*************9.....67799*************9 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PROSITE profile	PS50888	12.004	17	67	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	6.94E-11	21	86	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	2.6E-10	22	79	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Plant Ontology ? help Back to Top
PO Term	PO Category	PO Description
PO:0025017	anatomy	plant spore
PO:0030003	anatomy	protonema
PO:0030018	anatomy	gametophore

Sequence ? help Back to Top
Protein Sequence Length: 155 aa Download sequence Send to blast
MEYSKQDFIR VRARRGQATD SLSVAEWVRC EKIRKRMKYL QDLVPGCRKV TGKTDMLDEI 60 INYVQSLQCQ AESLSMKLGA VHPAPLDHLT LESLLSIEEV LQSQWSNILC TSDSTSSPEF 120 GLQQLPQQRG SMLDGHCNFD HFQANLGYSF NGSS*

Expression -- Description ? help Back to Top
Source	Description
Uniprot	TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, and stems. {ECO:0000269\|PubMed:12679534}.
Uniprot	TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, stems, and flowers. {ECO:0000269\|PubMed:12679534}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcriptional activator involved in cell elongation. Regulates the expression of a subset of genes involved in cell expansion by binding to the G-box motif. Binds to chromatin DNA of the FT gene and promotes its expression, and thus triggers flowering in response to blue light (PubMed:24130508). {ECO:0000269\|PubMed:23161888, ECO:0000269\|PubMed:24130508}.
UniProt	Transcription factor that binds DNA to G box 5'-CACGTG-3' and, to a lower extent, to E-box 5'-CANNTG-3' in vitro. Binds to chromatin DNA of the FT gene and promotes its expression, and thus triggers flowering in response to blue light. {ECO:0000269\|PubMed:18988809, ECO:0000269\|PubMed:24130508}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Accumulates strongly in response to blue light due to reduced preventing 26S proteasome-mediated degradation in an ADO1/ZTL and ADO2/LKP2 dependent manner, but levels decrease in the absence of blue light via 26S proteasome degradation (at protein level). {ECO:0000269\|PubMed:24101505}.

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_024358304.1	1e-113	transcription factor bHLH63-like
Swissprot	Q8GY61	3e-34	BH063_ARATH; Transcription factor bHLH63
Swissprot	Q9C670	6e-34	BH076_ARATH; Transcription factor bHLH76
TrEMBL	A9TVA7	1e-112	A9TVA7_PHYPA; Predicted protein
STRING	PP1S334_14V6.1	1e-108	(Physcomitrella patens)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT1G26260.3	9e-37	cryptochrome-interacting basic-helix-loop-helix 5

Link Out ? help Back to Top
Phytozome	Pp3c20_590V3.2.p
Entrez Gene	5945746

Publications ? help Back to Top
Rensing SA, et al. The Physcomitrella genome reveals evolutionary insights into the conquest of land by plants. Science, 2008. 319(5859): p. 64-9 [PMID:18079367] Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Ding Y, et al. Four distinct types of dehydration stress memory genes in Arabidopsis thaliana. BMC Plant Biol., 2013. 13: p. 229 [PMID:24377444] Yang D,Zhao W,Meng Y,Li H,Liu B A CIB1-LIKE transcription factor GmCIL10 from soybean positively regulates plant flowering. Sci China Life Sci, 2015. 58(3): p. 261-9 [PMID:25651969] Katsura Y, et al. An optogenetic system for interrogating the temporal dynamics of Akt. Sci Rep, 2015. 5: p. 14589 [PMID:26423353] Taslimi A, et al. Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. Nat. Chem. Biol., 2016. 12(6): p. 425-30 [PMID:27065233] Liu Q, et al. The Blue Light-Dependent Polyubiquitination and Degradation of Arabidopsis Cryptochrome2 Requires Multiple E3 Ubiquitin Ligases. Plant Cell Physiol., 2016. 57(10): p. 2175-2186 [PMID:27516416] Pathak GP, et al. Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res., 2017. 45(20): p. e167 [PMID:28431041] Duan L, et al. Understanding CRY2 interactions for optical control of intracellular signaling. Nat Commun, 2017. 8(1): p. 547 [PMID:28916751] Quejada JR, et al. Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1. Nucleic Acids Res., 2017. 45(20): p. e172 [PMID:29040770] Liu Y, et al. CIB1 and CO interact to mediate CRY2-dependent regulation of flowering. EMBO Rep., 2019. [PMID:30126927]