PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Psi001481

Organism

Picea sitchensis

Taxonomic ID

3332

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Acrogymnospermae; Pinidae; Pinales; Pinaceae; Picea

Family

bHLH

Protein Properties

Length: 457aa MW: 51096.1 Da PI: 5.9166

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
gnl\|UG\|Psi#S50073320	PU_unref	Unigene	View CDS
PUT-183a-Picea_sitchensis-15939	PU_ref	plantGDB	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	39.1	1.4e-12	248	299	1	55
CHHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 1 rrrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +r++h ++Er+RR+++N+ ++ Lr+l+P + + ++ + a+i A++++k+L+ Psi001481 248 QRMTHIAVERNRRKQMNEHLSVLRSLMPGS---YIQRGDQASIIGGAIDFVKELE 299 79**************************...9****************995 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
CDD	cd00083	2.30E-14	246	303	No hit	No description
PROSITE profile	PS50888	15.777	247	298	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamily	SSF47459	1.16E-16	247	315	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	6.3E-14	248	312	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	8.1E-10	248	299	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	3.9E-10	253	304	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 457 aa Download sequence Send to blast
MVLEALNSQV FNGTSMNQSP HEAGFAEMLH YPAERTTGMT FLQMLDGIAV NRDGVIRSFA 60 DDIEEKLAAP EQQLEMKEQR PWQHGFQQLL NFPLCHQIDD SAFMLPPEFS NQHATQYVSK 120 KSTGTVDNNN SHDFQQARIH HHQQLFEPES CITHVSESCS PIKTEACKDH SSSDAIEEGV 180 SSAALDPNSC ITTNSSSENG TGGGFVTSSM MPSSTGPSSQ LSTRMEVRDR RKRKRSKACK 240 NSEEVESQRM THIAVERNRR KQMNEHLSVL RSLMPGSYIQ RGDQASIIGG AIDFVKELEQ 300 LLQSLQAQKR KRECEEFGCS PNSPTPFNGF FLSPQYTSYS AQWNSRYAVE KTSFNDTGNE 360 LIAENKSAVA DIEVTMIETH ASIKILSQKR SGQLMKTIDK LQSLHMTILH LNITTIDQTV 420 LYSFNVKIED ECQLTSADEI ATAVHEIINS IHSNAIN

Sequence ? help Back to Top

Protein Sequence Length: 457 aa Download sequence Send to blast

MVLEALNSQV FNGTSMNQSP HEAGFAEMLH YPAERTTGMT FLQMLDGIAV NRDGVIRSFA  60
DDIEEKLAAP EQQLEMKEQR PWQHGFQQLL NFPLCHQIDD SAFMLPPEFS NQHATQYVSK  120
KSTGTVDNNN SHDFQQARIH HHQQLFEPES CITHVSESCS PIKTEACKDH SSSDAIEEGV  180
SSAALDPNSC ITTNSSSENG TGGGFVTSSM MPSSTGPSSQ LSTRMEVRDR RKRKRSKACK  240
NSEEVESQRM THIAVERNRR KQMNEHLSVL RSLMPGSYIQ RGDQASIIGG AIDFVKELEQ  300
LLQSLQAQKR KRECEEFGCS PNSPTPFNGF FLSPQYTSYS AQWNSRYAVE KTSFNDTGNE  360
LIAENKSAVA DIEVTMIETH ASIKILSQKR SGQLMKTIDK LQSLHMTILH LNITTIDQTV  420
LYSFNVKIED ECQLTSADEI ATAVHEIINS IHSNAIN

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	229	234	RRKRKR
2	248	259	RMTHIAVERNRR

Expression -- UniGene ? help Back to Top
UniGene ID	E-value	Expressed in
Psi.4674	0.0	shoot

Expression -- Description ? help Back to Top
Source	Description
Uniprot	DEVELOPMENTAL STAGE: Not expressed in meristemoids, but strongly expressed in guard mother cells (GMCs) and in young guard cells (at protein level) (PubMed:17088607). Expressed at the transition to terminal stomatal differentiation, just before and after the symmetric division of stomatal differentiation, being confined to late-stage GMC and to young, still differentiating guard cells (PubMed:24571519). {ECO:0000269\|PubMed:17088607, ECO:0000269\|PubMed:24571519}.
Uniprot	TISSUE SPECIFICITY: Resctricted to stomatal cell lineages (at protein level). Expressed in roots, leaves, stems, and flowers. {ECO:0000269\|PubMed:12679534, ECO:0000269\|PubMed:17088607}.

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator (PubMed:17088607, PubMed:17183265, PubMed:17183267). Together with MYB88 and MYB124, ensures that stomata contain just two guard cells (GCs) by enforcing a single symmetric precursor cell division before stomatal maturity (PubMed:24571519). Together with SPCH and MUTE, regulates the stomata formation. Required to promote differentiation and morphogenesis of stomatal guard cells and to halt proliferative divisions in their immediate precursors. Mediates the formation of stomata (PubMed:17088607, PubMed:17183265, PubMed:17183267). Prevents histone H3K27me3 marks and derepresses stem cell gene expression (PubMed:24654956). {ECO:0000269\|PubMed:17088607, ECO:0000269\|PubMed:17183265, ECO:0000269\|PubMed:17183267, ECO:0000269\|PubMed:24571519, ECO:0000269\|PubMed:24654956}.

Regulation -- Description ? help Back to Top
Source	Description
UniProt	INDUCTION: Inhibited by low relative humidity (LRH) via epigenetic CG methylation, thus leading to a reduced stomatal index. {ECO:0000269\|PubMed:22442411}.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_020532222.1	1e-106	transcription factor bHLH94 isoform X3
Refseq	XP_020532224.1	1e-106	transcription factor bHLH94 isoform X5
Swissprot	Q56YJ8	1e-70	FAMA_ARATH; Transcription factor FAMA
TrEMBL	B8LLL6	0.0	B8LLL6_PICSI; Uncharacterized protein
STRING	ERM96627	1e-105	(Amborella trichopoda)

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT3G24140.1	5e-60	bHLH family protein

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Matos JL, et al. Irreversible fate commitment in the Arabidopsis stomatal lineage requires a FAMA and RETINOBLASTOMA-RELATED module. Elife, 2015. [PMID:25303364] Chen L, et al. NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis. Development, 2016. 143(9): p. 1600-11 [PMID:26989174] Lee JH,Jung JH,Park CM Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis. Plant Cell, 2017. 29(11): p. 2817-2830 [PMID:29070509] Han X, et al. Jasmonate Negatively Regulates Stomatal Development in Arabidopsis Cotyledons. Plant Physiol., 2018. 176(4): p. 2871-2885 [PMID:29496884] Li X,Yang R,Chen H The Arabidopsis thaliana Mediator subunit MED8 regulates plant immunity to Botrytis Cinerea through interacting with the basic helix-loop-helix (bHLH) transcription factor FAMA. PLoS ONE, 2018. 13(3): p. e0193458 [PMID:29513733]