PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID Rsa1.0_01740.1_g00003.1
Organism
Raphanus sativus
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus
Family bHLH
Protein Properties Length: 622aa    MW: 68017 Da    PI: 6.0543
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
Rsa1.0_01740.1_g00003.1genomeRGDView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH23.21.3e-07350398254
                              HHHHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHH CS
                      HLH   2 rrahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksL 54 
                              +  +  rE+ RR+++N+ f eL  +l +      +K++Ka+iL+ + + +k+L
  Rsa1.0_01740.1_g00003.1 350 KSQKAGREKLRREKLNEHFVELGNVLDPE----RPKNDKATILTDTLQLLKDL 398
                              5566779******************9987....69***********9999988 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
HamapMF_0303850.0327348IPR028601Cytosolic Fe-S cluster assembly factor NUBP1/Nbp35
SuperFamilySSF525401.55E-5245242IPR027417P-loop containing nucleoside triphosphate hydrolase
Gene3DG3DSA:3.40.50.3005.0E-10351240IPR027417P-loop containing nucleoside triphosphate hydrolase
HamapMF_0204035.64953369IPR019591Mrp/NBP35 ATP-binding protein
PfamPF106096.7E-8158254IPR019591Mrp/NBP35 ATP-binding protein
CDDcd020374.61E-8661310No hitNo description
PROSITE patternPS012150166182IPR000808Mrp, conserved site
Gene3DG3DSA:3.40.50.3005.0E-103276335IPR027417P-loop containing nucleoside triphosphate hydrolase
SuperFamilySSF525401.55E-52278324IPR027417P-loop containing nucleoside triphosphate hydrolase
PROSITE profilePS5088812.773348398IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SuperFamilySSF474591.31E-11348412IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.106.3E-11348418IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000831.08E-6349402No hitNo description
PfamPF000101.9E-5350398IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003533.1E-5354404IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0016226Biological Processiron-sulfur cluster assembly
GO:0005829Cellular Componentcytosol
GO:0005524Molecular FunctionATP binding
GO:0042803Molecular Functionprotein homodimerization activity
GO:0051539Molecular Function4 iron, 4 sulfur cluster binding
Sequence ? help Back to Top
Protein Sequence    Length: 622 aa     Download sequence    Send to blast
MDNGEIPENA NEHCPGPQSE TAGKSDSCAG CPNQEVCATA PKGPDPDLVA VAERMSTVKH  60
KILVLSGKGG VGKSTFSAQL SFALAGMDHQ VGLMDIDICG PSMPKMLGLE GHEIHQSNLG  120
WSPVYVEDNL GVMSIGFMLP NSDEAVVWRG PRKNALIKQF LKDVYWGEID YLVVDAPPGT  180
SDEHISIVQY LQATGIDGAI IVTTPQEVSL IDVRKEVSFC KKVGVPVLGV VENMSGLCQP  240
LADVKFKKVH SESGSSVDVT QEVISCLRKN APELVNVLAY SEVFDRSGGG AERMCREMGV  300
PFLGSVPLDP QLCRAAEQGK SCFEGENKCS VSAPALNSFG RRVMDVSARK SQKAGREKLR  360
REKLNEHFVE LGNVLDPERP KNDKATILTD TLQLLKDLTS EVTKLKSEYT ALTDESREEK  420
NDLREEKTSL KSDIENLNLQ YQQRLRSMSP WGAAMDHTVM MAPPPSFPYP MPMAMPPGSI  480
PMHHHPPMPS YTYFGNQNPS MMPAPYMPYM QPPNTVVEQQ SVHIPQNNRS REPRTKVSRE  540
SRSDKAEDSS DVATQLELKT PGSTSDKDTL QRPEKSKRCK RNSNNNSVEE SSHSSKCSSS  600
PSVRDNTSSS SVAGGLKPDD AK
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
6g2g_A2e-71553203243Cytosolic Fe-S cluster assembly factor CFD1
6g2g_B2e-71553203243Cytosolic Fe-S cluster assembly factor CFD1
Search in ModeBase
Functional Description ? help Back to Top
Source Description
UniProtComponent of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development. {ECO:0000255|HAMAP-Rule:MF_03038, ECO:0000269|PubMed:18957412, ECO:0000269|PubMed:19084504}.
Cis-element ? help Back to Top
SourceLink
PlantRegMapRsa1.0_01740.1_g00003.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_013648078.10.0cytosolic Fe-S cluster assembly factor NBP35
SwissprotQ8H1Q20.0NBP35_ARATH; Cytosolic Fe-S cluster assembly factor NBP35
TrEMBLA0A3P5ZFK80.0A0A3P5ZFK8_BRACM; Cytosolic Fe-S cluster assembly factor NBP35
STRINGBra038236.1-P0.0(Brassica rapa)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT3G19860.21e-103bHLH family protein
Publications ? help Back to Top
  1. Godman J,Balk J
    Genome analysis of Chlamydomonas reinhardtii reveals the existence of multiple, compartmentalized iron-sulfur protein assembly machineries of different evolutionary origins.
    Genetics, 2008. 179(1): p. 59-68
    [PMID:18493040]
  2. Bych K, et al.
    The essential cytosolic iron-sulfur protein Nbp35 acts without Cfd1 partner in the green lineage.
    J. Biol. Chem., 2008. 283(51): p. 35797-804
    [PMID:18957412]
  3. Kohbushi H, et al.
    Arabidopsis cytosolic Nbp35 homodimer can assemble both [2Fe-2S] and [4Fe-4S] clusters in two distinct domains.
    Biochem. Biophys. Res. Commun., 2009. 378(4): p. 810-5
    [PMID:19084504]
  4. Bastow EL,Bych K,Crack JC,Le Brun NE,Balk J
    NBP35 interacts with DRE2 in the maturation of cytosolic iron-sulphur proteins in Arabidopsis thaliana.
    Plant J., 2017. 89(3): p. 590-600
    [PMID:27801963]