PlantTFDB - Plant Transcription Factor Database @ CBI, PKU

PlantRegMap/PlantTFDB v5.0

Plant Transcription Factor Database

Transcription Factor Information

Basic Information | Signature Domain | Sequence |

Basic Information? help Back to Top

TF ID

Thhalv10028584m

Common Name

EUTSA_v10028584mg

Organism

Eutrema salsugineum

Taxonomic ID

72664

Taxonomic Lineage

cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Eutremeae; Eutrema

Family

bHLH

Protein Properties

Length: 515aa MW: 58892.5 Da PI: 5.3308

Description

bHLH family protein

Gene Model

Gene Model ID	Type	Source	Coding Sequence
Thhalv10028584m	genome	JGI	View CDS

Signature Domain? help Back to Top

No.	Domain	Score	E-value	Start	End	HMM Start	HMM End
1	HLH	44.2	3.4e-14	360	406	4	55
HHHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS HLH 4 ahnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 +h +ErrRR+++N++f +Lr+l+P +K++Ka+iL ++eY+++L+ Thhalv10028584m 360 NHVVAERRRREKLNERFITLRSLVPFV-----TKMDKASILGDTIEYVNHLR 406 6999********************8.....8***************95 PP

Protein Features ? help Back to Top

Database	Entry ID	E-value	Start	End	InterPro ID	Description
Pfam	PF14215	1.6E-52	20	196	IPR025610	Transcription factor MYC/MYB N-terminal
PROSITE profile	PS50888	17.485	356	405	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
CDD	cd00083	7.41E-14	359	410	No hit	No description
SuperFamily	SSF47459	2.62E-18	359	418	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Pfam	PF00010	1.3E-11	360	405	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
Gene3D	G3DSA:4.10.280.10	6.3E-19	360	419	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
SMART	SM00353	8.8E-18	362	411	IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain
PROSITE profile	PS51671	8.505	445	514	IPR002912	ACT domain

Gene Ontology ? help Back to Top
GO Term	GO Category	GO Description
GO:0010026	Biological Process	trichome differentiation
GO:0010214	Biological Process	seed coat development
GO:2000029	Biological Process	regulation of proanthocyanidin biosynthetic process
GO:0000976	Molecular Function	transcription regulatory region sequence-specific DNA binding
GO:0016597	Molecular Function	amino acid binding
GO:0046983	Molecular Function	protein dimerization activity

Sequence ? help Back to Top
Protein Sequence Length: 515 aa Download sequence Send to blast
MDESSIIPAE KVIGAERREL QGLLKAAVQS VEWTYSIFWQ LCPQQRVLVW ENGYYNGAIK 60 TRKTTQPAEV TAEEAALERS QQLRELYETL LAGESSSETR ACTALSPEDL TETEWFYLMC 120 VSFSFPPPSG MPGKAYARRK HVWLSGANEV DSKIFSRAIL AKTVVCIPML DGVVELGTTN 180 KVKEDVEFVE HIKSFFHNHP KSNPKPALSE HSINVDQHEE DEEEEEEVEE EMTMSEEIRI 240 GSPDDDDVSN QNLLSDFHIE STHTLDTHMD MMNLMEEGGN YSQTVSTLLM SQPTSLLSDS 300 VSTSSYVQSS FHTWRVENVK EHQQVEKASS SQWMLKHIIL RVPFLHDNNK DKRLPREELN 360 HVVAERRRRE KLNERFITLR SLVPFVTKMD KASILGDTIE YVNHLRKRVH ELETIHHGQE 420 HKRLRTCKRK TSEEVEVSII ESDALLEMRC EYRDGLLLDI LQALKELGIE TTGVQTAVNE 480 HDFEAEIRAK VRGKKPSIAE VKRAVHQIIS NTKL*

Sequence ? help Back to Top

Protein Sequence Length: 515 aa Download sequence Send to blast

MDESSIIPAE KVIGAERREL QGLLKAAVQS VEWTYSIFWQ LCPQQRVLVW ENGYYNGAIK  60
TRKTTQPAEV TAEEAALERS QQLRELYETL LAGESSSETR ACTALSPEDL TETEWFYLMC  120
VSFSFPPPSG MPGKAYARRK HVWLSGANEV DSKIFSRAIL AKTVVCIPML DGVVELGTTN  180
KVKEDVEFVE HIKSFFHNHP KSNPKPALSE HSINVDQHEE DEEEEEEVEE EMTMSEEIRI  240
GSPDDDDVSN QNLLSDFHIE STHTLDTHMD MMNLMEEGGN YSQTVSTLLM SQPTSLLSDS  300
VSTSSYVQSS FHTWRVENVK EHQQVEKASS SQWMLKHIIL RVPFLHDNNK DKRLPREELN  360
HVVAERRRRE KLNERFITLR SLVPFVTKMD KASILGDTIE YVNHLRKRVH ELETIHHGQE  420
HKRLRTCKRK TSEEVEVSII ESDALLEMRC EYRDGLLLDI LQALKELGIE TTGVQTAVNE  480
HDFEAEIRAK VRGKKPSIAE VKRAVHQIIS NTKL*

3D Structure ? help Back to Top

PDB ID	Evalue	Query Start	Query End	Hit Start	Hit End	Description
4rqw_A	3e-19	17	197	10	193	Transcription factor MYC3
4rqw_B	3e-19	17	197	10	193	Transcription factor MYC3
4rs9_A	3e-19	17	197	10	193	Transcription factor MYC3
4yz6_A	3e-19	17	197	10	193	Transcription factor MYC3
Search in ModeBase

Nucleic Localization Signal ? help Back to Top

No.	Start	End	Sequence
1	364	369	ERRRRE
2	365	370	RRRREK

Functional Description ? help Back to Top
Source	Description
UniProt	Transcription activator, when associated with MYB75/PAP1 or MYB90/PAP2. Involved in the control of flavonoid pigmentation. Plays a key role in regulating leucoanthocyanidin reductase (BANYULS) and dihydroflavonol-4-reductase (DFR). Not required for leucoanthocyanidin dioxygenase (LDOX) expression. {ECO:0000269\|PubMed:15361138}.

Cis-element ? help Back to Top
Source	Link
PlantRegMap	Thhalv10028584m

Regulation -- PlantRegMap ? help Back to Top
Source	Upstream Regulator	Target Gene
PlantRegMap	Retrieve	-

Annotation -- Nucleotide ? help Back to Top
Source	Hit ID	E-value	Description
GenBank	AJ277509	0.0	AJ277509.1 Arabidopsis thaliana mRNA for transparent testa 8 bHLH protein, (tt8 gene).
GenBank	DQ446813	0.0	DQ446813.1 Arabidopsis thaliana clone pENTR221-At4g09820 basic helix-loop-helix family protein (At4g09820) mRNA, complete cds.
GenBank	DQ653187	0.0	DQ653187.1 Arabidopsis thaliana clone 0000017471_0000012221 unknown mRNA.

Annotation -- Protein ? help Back to Top
Source	Hit ID	E-value	Description
Refseq	XP_024009789.1	0.0	transcription factor TT8
Swissprot	Q9FT81	0.0	TT8_ARATH; Transcription factor TT8
TrEMBL	V4LFR2	0.0	V4LFR2_EUTSA; Uncharacterized protein
STRING	XP_006397140.1	0.0	(Eutrema salsugineum)

Orthologous Group ? help Back to Top
Lineage	Orthologous Group ID	Taxa Number	Gene Number
Malvids	OGEM9422	28	36

Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID	E-value	Description
AT4G09820.1	0.0	bHLH family protein

Link Out ? help Back to Top
Phytozome	Thhalv10028584m
Entrez Gene	18014332

Publications ? help Back to Top
Skinner MK,Rawls A,Wilson-Rawls J,Roalson EH Basic helix-loop-helix transcription factor gene family phylogenetics and nomenclature. Differentiation, 2010. 80(1): p. 1-8 [PMID:20219281] Kim K,Jiang K,Teng SL,Feldman LJ,Huang H Using biologically interrelated experiments to identify pathway genes in Arabidopsis. Bioinformatics, 2012. 28(6): p. 815-22 [PMID:22271267] Liu Z,Shi MZ,Xie DY Regulation of anthocyanin biosynthesis in Arabidopsis thaliana red pap1-D cells metabolically programmed by auxins. Planta, 2014. 239(4): p. 765-81 [PMID:24370633] Ranocha P,Francoz E,Burlat V,Dunand C Expression of PRX36, PMEI6 and SBT1.7 is controlled by complex transcription factor regulatory networks for proper seed coat mucilage extrusion. Plant Signal Behav, 2014. 9(11): p. e977734 [PMID:25531128] Zhu Z, et al. Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation. J. Exp. Bot., 2015. 66(13): p. 3775-89 [PMID:25911741] Zhu Q, et al. Ectopic Expression of the Coleus R2R3 MYB-Type Proanthocyanidin Regulator Gene SsMYB3 Alters the Flower Color in Transgenic Tobacco. PLoS ONE, 2015. 10(10): p. e0139392 [PMID:26448466] Li P, et al. Regulation of anthocyanin and proanthocyanidin biosynthesis by Medicago truncatula bHLH transcription factor MtTT8. New Phytol., 2016. 210(3): p. 905-21 [PMID:26725247] Bulgakov VP,Veremeichik GN,Grigorchuk VP,Rybin VG,Shkryl YN The rolB gene activates secondary metabolism in Arabidopsis calli via selective activation of genes encoding MYB and bHLH transcription factors. Plant Physiol. Biochem., 2016. 102: p. 70-9 [PMID:26913794] Petridis A,Döll S,Nichelmann L,Bilger W,Mock HP Arabidopsis thaliana G2-LIKE FLAVONOID REGULATOR and BRASSINOSTEROID ENHANCED EXPRESSION1 are low-temperature regulators of flavonoid accumulation. New Phytol., 2016. 211(3): p. 912-25 [PMID:27125220] Rai A, et al. Coordinate Regulation of Metabolite Glycosylation and Stress Hormone Biosynthesis by TT8 in Arabidopsis. Plant Physiol., 2016. 171(4): p. 2499-515 [PMID:27432888] Li Y, et al. Two IIIf Clade-bHLHs from Freesia hybrida Play Divergent Roles in Flavonoid Biosynthesis and Trichome Formation when Ectopically Expressed in Arabidopsis. Sci Rep, 2016. 6: p. 30514 [PMID:27465838] Mahmood K,Xu Z,El-Kereamy A,Casaretto JA,Rothstein SJ The Arabidopsis Transcription Factor ANAC032 Represses Anthocyanin Biosynthesis in Response to High Sucrose and Oxidative and Abiotic Stresses. Front Plant Sci, 2016. 7: p. 1548 [PMID:27790239] Zou B, et al. Calmodulin-binding protein CBP60g functions as a negative regulator in Arabidopsis anthocyanin accumulation. PLoS ONE, 2017. 12(3): p. e0173129 [PMID:28253311] Chen K,Liu H,Lou Q,Liu Y Ectopic Expression of the Grape Hyacinth (Muscari armeniacum) R2R3-MYB Transcription Factor Gene, MaAN2, Induces Anthocyanin Accumulation in Tobacco. Front Plant Sci, 2017. 8: p. 965 [PMID:28642775] Kataya ARA, et al. PLATINUM SENSITIVE 2 LIKE impacts growth, root morphology, seed set, and stress responses. PLoS ONE, 2017. 12(7): p. e0180478 [PMID:28678890] Ezer D, et al. The G-Box Transcriptional Regulatory Code in Arabidopsis. Plant Physiol., 2017. 175(2): p. 628-640 [PMID:28864470] Feng K, et al. An R2R3-MYB transcription factor, OjMYB1, functions in anthocyanin biosynthesis in Oenanthe javanica. Planta, 2018. 247(2): p. 301-315 [PMID:28965159] Wen J, et al. The C-terminal domains of Arabidopsis GL3/EGL3/TT8 interact with JAZ proteins and mediate dimeric interactions. Plant Signal Behav, 2018. 13(1): p. e1422460 [PMID:29293407]