PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID XP_016453709.1
Common NameERF3, ERF-3, ERF4
Organism
Nicotiana tabacum
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana
Family ERF
Protein Properties Length: 225aa    MW: 24262 Da    PI: 8.9608
Description ERF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
XP_016453709.1genomeNCBIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1AP266.17e-212675255
             AP2  2 gykGVrwdkkrgrWvAeIrdpsengkrkrfslgkfgtaeeAakaaiaarkkleg 55
                    +y+GVr ++ +gr++AeIrdp   gk+ r++lg+f+taeeAaka++ a+++++g
  XP_016453709.1 26 HYRGVRKRP-WGRYAAEIRDP---GKKSRVWLGTFDTAEEAAKAYDTAAREFRG 75
                    6********.**********8...557*************************98 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF541711.77E-222683IPR016177DNA-binding domain
PROSITE profilePS5103224.1572683IPR001471AP2/ERF domain
SMARTSM003803.9E-402689IPR001471AP2/ERF domain
CDDcd000181.57E-312683No hitNo description
PfamPF008475.7E-142675IPR001471AP2/ERF domain
Gene3DG3DSA:3.30.730.107.5E-332683IPR001471AP2/ERF domain
PRINTSPR003672.3E-112738IPR001471AP2/ERF domain
PRINTSPR003672.3E-114965IPR001471AP2/ERF domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0006952Biological Processdefense response
GO:0009873Biological Processethylene-activated signaling pathway
GO:0005634Cellular Componentnucleus
GO:0016020Cellular Componentmembrane
GO:0003677Molecular FunctionDNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
Sequence ? help Back to Top
Protein Sequence    Length: 225 aa     Download sequence    Send to blast
MAVKNKVSNG NLKGGNVKTD GVKEVHYRGV RKRPWGRYAA EIRDPGKKSR VWLGTFDTAE  60
EAAKAYDTAA REFRGPKAKT NFPSPTENQS PSHSSTVESS SGENGVHAPP HAPLELDLTR  120
RLGSVAADGG DNCRRSGEVG YPIFHQQPTV AVLPNGQPVL LFDSLWRAGV VNRPQPYHVT  180
PMGFNGVNAG VGPTVSDSSS AVEENQYDGK RGIDLDLNLA PPMEF
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
1gcc_A2e-212683260ETHYLENE RESPONSIVE ELEMENT BINDING FACTOR 1
2gcc_A2e-212683563ATERF1
3gcc_A2e-212683563ATERF1
Search in ModeBase
Functional Description ? help Back to Top
Source Description
UniProtActs as a transcriptional repressor. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways mediated by ethylene, that seems to depend on a protein kinase/phosphatase cascade, and to be influenced by methyl-jasmonate. May regulate other AtERFs (By similarity). {ECO:0000250, ECO:0000269|PubMed:10652129, ECO:0000269|PubMed:10792818, ECO:0000269|PubMed:12654868, ECO:0000269|PubMed:7756828, ECO:0000269|Ref.2}.
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00542DAPTransfer from AT5G44210Download
Motif logo
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Strongly induced by ethephon (ethylene-releasing compound) in buds and to lower extent in leaves. Strongly induced by cycloheximide and mechanical stimuli. Wounding leads to a both local and systemical transient expression, independently of ethylene, and through a de-novo-protein-synthesis-independent regulation. Not influenced by methyl-jasmonate. Induction by purified xylanase from Trichoderma viride (TvX) and another elicitor from Phytophthora infestans (PiE), that appears to be mediated by a protein kinase cascade, and to be negatively regulated by protein phosphatases. {ECO:0000269|PubMed:10652129, ECO:0000269|PubMed:12090623, ECO:0000269|PubMed:7756828, ECO:0000269|Ref.2}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankTOBBY4B0.0D38124.1 Nicotiana tabacum EREBP-3 mRNA for ethylene-responsive element binding protein, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_001312182.11e-166ethylene-responsive transcription factor 4
SwissprotQ404771e-167ERF4_TOBAC; Ethylene-responsive transcription factor 4
TrEMBLA0A1S3YN671e-165A0A1S3YN67_TOBAC; ethylene-responsive transcription factor 4
STRINGXP_009595390.11e-164(Nicotiana tomentosiformis)
STRINGXP_009609806.11e-164(Nicotiana tomentosiformis)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
AsteridsOGEA21241165
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT5G44210.18e-29erf domain protein 9
Publications ? help Back to Top
  1. Yamamoto S,Suzuki K,Shinshi H
    Elicitor-responsive, ethylene-independent activation of GCC box-mediated transcription that is regulated by both protein phosphorylation and dephosphorylation in cultured tobacco cells.
    Plant J., 1999. 20(5): p. 571-9
    [PMID:10652129]
  2. Ohta M,Ohme-Takagi M,Shinshi H
    Three ethylene-responsive transcription factors in tobacco with distinct transactivation functions.
    Plant J., 2000. 22(1): p. 29-38
    [PMID:10792818]
  3. Koyama T,Kitajima S,Sato F
    Expression of PR-5d and ERF genes in cultured tobacco cells and their NaCl stress-response.
    Biosci. Biotechnol. Biochem., 2001. 65(5): p. 1270-3
    [PMID:11440157]
  4. Nishiuchi T,Suzuki K,Kitajima S,Sato F,Shinshi H
    Wounding activates immediate early transcription of genes for ERFs in tobacco plants.
    Plant Mol. Biol., 2002. 49(5): p. 473-82
    [PMID:12090623]
  5. Koyama T, et al.
    Isolation of tobacco ubiquitin-conjugating enzyme cDNA in a yeast two-hybrid system with tobacco ERF3 as bait and its characterization of specific interaction.
    J. Exp. Bot., 2003. 54(385): p. 1175-81
    [PMID:12654868]
  6. Ogata T, et al.
    Involvement of NtERF3 in the cell death signalling pathway mediated by SIPK/WIPK and WRKY1 in tobacco plants.
    Plant Biol (Stuttg), 2015. 17(5): p. 962-72
    [PMID:25996234]
  7. Ohme-Takagi M,Shinshi H
    Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element.
    Plant Cell, 1995. 7(2): p. 173-82
    [PMID:7756828]