PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID XP_016493410.1
Organism
Nicotiana tabacum
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana
Family ARF
Protein Properties Length: 839aa    MW: 93894.5 Da    PI: 6.3186
Description ARF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
XP_016493410.1genomeNCBIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1B372.64.7e-23127228199
                     EEEE-..-HHHHTT-EE--HHH.HTT.......---..--SEEEEEETTS-EEEEEE..EEETTEEEE-TTHHHHHHHHT--TT-EEEEEE-SSS CS
              B3   1 ffkvltpsdvlksgrlvlpkkfaeeh.......ggkkeesktltledesgrsWevkliyrkksgryvltkGWkeFvkangLkegDfvvFkldgrs 88 
                     f+k+lt sd++++g +++p++ ae+        +   +  ++l+ +d++  +W++++i+r++++r++lt+GW+ Fv+a++L +gD+v+F   +++
  XP_016493410.1 127 FCKTLTASDTSTHGGFSVPRRAAEKVfppldfsQT--PPAQELIARDLHDVEWKFRHIFRGQPKRHLLTTGWSVFVSAKRLVAGDSVLFI--WNE 217
                     99*****************************7444..44459************************************************..888 PP

                     EE..EEEEE-S CS
              B3  89 efelvvkvfrk 99 
                     + +l+++++r+
  XP_016493410.1 218 KNQLLLGIRRA 228
                     9999***9986 PP
2Auxin_resp117.51.1e-38253336183
      Auxin_resp   1 aahaastksvFevvYnPrastseFvvkvekvekalk.vkvsvGmRfkmafetedsserrlsGtvvgvsdldpvrWpnSkWrsLk 83 
                     aahaa+t+s F+v++nPras+seFv++++k+ ka++ ++vsvGmRf+m fete+ss rr++Gt++g+ dldpvrWpnS+Wrs+k
  XP_016493410.1 253 AAHAAATNSCFTVFFNPRASPSEFVIPLSKYIKAVYhTRVSVGMRFRMLFETEESSVRRYMGTITGIGDLDPVRWPNSHWRSVK 336
                     79*********************************9789******************************************985 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF1019364.19E-49111256IPR015300DNA-binding pseudobarrel domain
Gene3DG3DSA:2.40.330.101.1E-40120242IPR015300DNA-binding pseudobarrel domain
CDDcd100172.57E-21126227No hitNo description
PfamPF023622.9E-21127228IPR003340B3 DNA binding domain
PROSITE profilePS5086311.998127229IPR003340B3 DNA binding domain
SMARTSM010191.0E-23127229IPR003340B3 DNA binding domain
PfamPF065075.1E-34253336IPR010525Auxin response factor
PfamPF023098.8E-8704798IPR033389AUX/IAA domain
PROSITE profilePS5174520.753712796IPR000270PB1 domain
SuperFamilySSF542771.5E-6713786No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009725Biological Processresponse to hormone
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0005515Molecular Functionprotein binding
Sequence ? help Back to Top
Protein Sequence    Length: 839 aa     Download sequence    Send to blast
MKLSTSGMGQ QAHEGEKKCL NSELWHACAG PLVCLPTVGS RVVYFPQGHS EQVAATTNKE  60
VDAHIPNYPN LPPQLICQLH NVTMHADVET DEVYAQMTLQ PLTPEEQKDT YLPVEFGIPS  120
KQPTNYFCKT LTASDTSTHG GFSVPRRAAE KVFPPLDFSQ TPPAQELIAR DLHDVEWKFR  180
HIFRGQPKRH LLTTGWSVFV SAKRLVAGDS VLFIWNEKNQ LLLGIRRAIR PQTVMPSSVL  240
SSDSMHIGLL AAAAHAAATN SCFTVFFNPR ASPSEFVIPL SKYIKAVYHT RVSVGMRFRM  300
LFETEESSVR RYMGTITGIG DLDPVRWPNS HWRSVKVGWD ESTAGERQPR VSLWEIEPLT  360
TFPMYPSLFP LRLKRPLYPG TSSYQDSNNE AINRMAWLRG NSGELGPHSV NLQSFGMLPW  420
MQQRVDSTIL PNDLNQHYQA MLTTGLQSYG SGDLLKQQLM QFQQPVQYLQ HASSDNSILQ  480
QQQVMQQAVH QHMLPAQTQM LSENLQRQQQ QQPNNQSEEQ AHQHAYQEAF QIPHDQLQQR  540
QQPPNIPSSF SKEDFADLNS KFSASVAPSG VQNMLGSFCS EGSSNSLNIN RTGQSVVIEQ  600
PSQQSWISKF MQSQLNTCSN SSPLPTYGKD TLNSRENCSL DTQNQALFGA SIDSSGLLLP  660
ITVSNVTTTC ADISSMPLGM NSGYQNSLYG YVQDSSELLH NAGQNDPPNA SHSFVKVYKS  720
GCVGRSLDIT QFHSYHELRR ELGQMFGIEG FLEDPQRSGW QLVFVDREND ILLLGDDPWE  780
AFVNNVWYIK ILSPEDVQKL GKEEAKSLNR GAVERMSSST SADDRDLVSG MPSLGSLEY
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4ldu_A1e-1611635747388Auxin response factor 5
Search in ModeBase
Functional Description ? help Back to Top
Source Description
UniProtAuxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Regulates both stamen and gynoecium maturation. Promotes jasmonic acid production. Partially redundant with ARF6. Involved in fruit initiation. Acts as an inhibitor to stop further carpel development in the absence of fertilization and the generation of signals required to initiate fruit and seed development. {ECO:0000269|PubMed:12036261, ECO:0000269|PubMed:16107481, ECO:0000269|PubMed:16829592}.
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Repressed by miR167. {ECO:0000269|PubMed:17021043}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_016493410.10.0PREDICTED: auxin response factor 8-like isoform X2
SwissprotQ9FGV10.0ARFH_ARATH; Auxin response factor 8
TrEMBLA0A1S4BWY00.0A0A1S4BWY0_TOBAC; Auxin response factor
STRINGXP_009631563.10.0(Nicotiana tomentosiformis)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT5G37020.10.0auxin response factor 8
Publications ? help Back to Top
  1. Liu N, et al.
    Down-regulation of AUXIN RESPONSE FACTORS 6 and 8 by microRNA 167 leads to floral development defects and female sterility in tomato.
    J. Exp. Bot., 2014. 65(9): p. 2507-20
    [PMID:24723401]
  2. Minato N, et al.
    The phytoplasmal virulence factor TENGU causes plant sterility by downregulating of the jasmonic acid and auxin pathways.
    Sci Rep, 2014. 4: p. 7399
    [PMID:25492247]
  3. Ortega-Amaro MA, et al.
    Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance.
    Front Plant Sci, 2014. 5: p. 782
    [PMID:25653657]
  4. Wang Y, et al.
    MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development.
    Plant Physiol., 2015. 168(3): p. 984-99
    [PMID:25941314]
  5. Barik S, et al.
    Coevolution Pattern and Functional Conservation or Divergence of miR167s and their targets across Diverse Plant Species.
    Sci Rep, 2015. 5: p. 14611
    [PMID:26459056]
  6. Pashkovskiy PP,Kartashov AV,Zlobin IE,Pogosyan SI,Kuznetsov VV
    Blue light alters miR167 expression and microRNA-targeted auxin response factor genes in Arabidopsis thaliana plants.
    Plant Physiol. Biochem., 2016. 104: p. 146-54
    [PMID:27031426]
  7. Mlotshwa S,Pruss GJ,MacArthur JL,Reed JW,Vance V
    Developmental Defects Mediated by the P1/HC-Pro Potyviral Silencing Suppressor Are Not Due to Misregulation of AUXIN RESPONSE FACTOR 8.
    Plant Physiol., 2016. 172(3): p. 1853-1861
    [PMID:27688620]
  8. Wójcikowska B,Gaj MD
    Expression profiling of AUXIN RESPONSE FACTOR genes during somatic embryogenesis induction in Arabidopsis.
    Plant Cell Rep., 2017. 36(6): p. 843-858
    [PMID:28255787]
  9. Zhang GZ, et al.
    Ectopic expression of UGT84A2 delayed flowering by indole-3-butyric acid-mediated transcriptional repression of ARF6 and ARF8 genes in Arabidopsis.
    Plant Cell Rep., 2017. 36(12): p. 1995-2006
    [PMID:29027578]
  10. Zheng K, et al.
    Involvement of PACLOBUTRAZOL RESISTANCE6/KIDARI, an Atypical bHLH Transcription Factor, in Auxin Responses in Arabidopsis.
    Front Plant Sci, 2017. 8: p. 1813
    [PMID:29114256]
  11. Liu Z, et al.
    ARF2-ARF4 and ARF5 are Essential for Female and Male Gametophyte Development in Arabidopsis.
    Plant Cell Physiol., 2018. 59(1): p. 179-189
    [PMID:29145642]
  12. Ghelli R, et al.
    A Newly Identified Flower-Specific Splice Variant of AUXIN RESPONSE FACTOR8 Regulates Stamen Elongation and Endothecium Lignification in Arabidopsis.
    Plant Cell, 2018. 30(3): p. 620-637
    [PMID:29514943]