PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID XP_016493411.1
Organism
Nicotiana tabacum
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana
Family ARF
Protein Properties Length: 806aa    MW: 90232.3 Da    PI: 6.259
Description ARF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
XP_016493411.1genomeNCBIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1B372.74.5e-2394195199
                     EEEE-..-HHHHTT-EE--HHH.HTT.......---..--SEEEEEETTS-EEEEEE..EEETTEEEE-TTHHHHHHHHT--TT-EEEEEE-SSS CS
              B3   1 ffkvltpsdvlksgrlvlpkkfaeeh.......ggkkeesktltledesgrsWevkliyrkksgryvltkGWkeFvkangLkegDfvvFkldgrs 88 
                     f+k+lt sd++++g +++p++ ae+        +   +  ++l+ +d++  +W++++i+r++++r++lt+GW+ Fv+a++L +gD+v+F   +++
  XP_016493411.1  94 FCKTLTASDTSTHGGFSVPRRAAEKVfppldfsQT--PPAQELIARDLHDVEWKFRHIFRGQPKRHLLTTGWSVFVSAKRLVAGDSVLFI--WNE 184
                     99*****************************7444..44459************************************************..888 PP

                     EE..EEEEE-S CS
              B3  89 efelvvkvfrk 99 
                     + +l+++++r+
  XP_016493411.1 185 KNQLLLGIRRA 195
                     9999***9986 PP
2Auxin_resp117.61.1e-38220303183
      Auxin_resp   1 aahaastksvFevvYnPrastseFvvkvekvekalk.vkvsvGmRfkmafetedsserrlsGtvvgvsdldpvrWpnSkWrsLk 83 
                     aahaa+t+s F+v++nPras+seFv++++k+ ka++ ++vsvGmRf+m fete+ss rr++Gt++g+ dldpvrWpnS+Wrs+k
  XP_016493411.1 220 AAHAAATNSCFTVFFNPRASPSEFVIPLSKYIKAVYhTRVSVGMRFRMLFETEESSVRRYMGTITGIGDLDPVRWPNSHWRSVK 303
                     79*********************************9789******************************************985 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF1019363.92E-4978223IPR015300DNA-binding pseudobarrel domain
Gene3DG3DSA:2.40.330.101.0E-4087209IPR015300DNA-binding pseudobarrel domain
CDDcd100172.38E-2193194No hitNo description
PfamPF023622.7E-2194195IPR003340B3 DNA binding domain
SMARTSM010191.0E-2394196IPR003340B3 DNA binding domain
PROSITE profilePS5086311.99894196IPR003340B3 DNA binding domain
PfamPF065074.9E-34220303IPR010525Auxin response factor
PfamPF023098.4E-8671765IPR033389AUX/IAA domain
PROSITE profilePS5174520.753679763IPR000270PB1 domain
SuperFamilySSF542771.44E-6680753No hitNo description
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009725Biological Processresponse to hormone
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0005515Molecular Functionprotein binding
Sequence ? help Back to Top
Protein Sequence    Length: 806 aa     Download sequence    Send to blast
MKLSTSGMGQ QAHEGEKKCL NSELWHACAG PLVCLPTVGS RVVYFPQGHS EQADVETDEV  60
YAQMTLQPLT PEEQKDTYLP VEFGIPSKQP TNYFCKTLTA SDTSTHGGFS VPRRAAEKVF  120
PPLDFSQTPP AQELIARDLH DVEWKFRHIF RGQPKRHLLT TGWSVFVSAK RLVAGDSVLF  180
IWNEKNQLLL GIRRAIRPQT VMPSSVLSSD SMHIGLLAAA AHAAATNSCF TVFFNPRASP  240
SEFVIPLSKY IKAVYHTRVS VGMRFRMLFE TEESSVRRYM GTITGIGDLD PVRWPNSHWR  300
SVKVGWDEST AGERQPRVSL WEIEPLTTFP MYPSLFPLRL KRPLYPGTSS YQDSNNEAIN  360
RMAWLRGNSG ELGPHSVNLQ SFGMLPWMQQ RVDSTILPND LNQHYQAMLT TGLQSYGSGD  420
LLKQQLMQFQ QPVQYLQHAS SDNSILQQQQ VMQQAVHQHM LPAQTQMLSE NLQRQQQQQP  480
NNQSEEQAHQ HAYQEAFQIP HDQLQQRQQP PNIPSSFSKE DFADLNSKFS ASVAPSGVQN  540
MLGSFCSEGS SNSLNINRTG QSVVIEQPSQ QSWISKFMQS QLNTCSNSSP LPTYGKDTLN  600
SRENCSLDTQ NQALFGASID SSGLLLPITV SNVTTTCADI SSMPLGMNSG YQNSLYGYVQ  660
DSSELLHNAG QNDPPNASHS FVKVYKSGCV GRSLDITQFH SYHELRRELG QMFGIEGFLE  720
DPQRSGWQLV FVDRENDILL LGDDPWEAFV NNVWYIKILS PEDVQKLGKE EAKSLNRGAV  780
ERMSSSTSAD DRDLVSGMPS LGSLEY
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4ldu_A1e-1371632447388Auxin response factor 5
Search in ModeBase
Functional Description ? help Back to Top
Source Description
UniProtAuxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Regulates both stamen and gynoecium maturation. Promotes jasmonic acid production. Partially redundant with ARF6. Involved in fruit initiation. Acts as an inhibitor to stop further carpel development in the absence of fertilization and the generation of signals required to initiate fruit and seed development. {ECO:0000269|PubMed:12036261, ECO:0000269|PubMed:16107481, ECO:0000269|PubMed:16829592}.
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Repressed by miR167. {ECO:0000269|PubMed:17021043}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_016493411.10.0PREDICTED: auxin response factor 8-like isoform X3
SwissprotQ9FGV10.0ARFH_ARATH; Auxin response factor 8
TrEMBLA0A1S4BWY20.0A0A1S4BWY2_TOBAC; Auxin response factor
STRINGXP_009631563.10.0(Nicotiana tomentosiformis)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT5G37020.10.0auxin response factor 8
Publications ? help Back to Top
  1. Liu N, et al.
    Down-regulation of AUXIN RESPONSE FACTORS 6 and 8 by microRNA 167 leads to floral development defects and female sterility in tomato.
    J. Exp. Bot., 2014. 65(9): p. 2507-20
    [PMID:24723401]
  2. Minato N, et al.
    The phytoplasmal virulence factor TENGU causes plant sterility by downregulating of the jasmonic acid and auxin pathways.
    Sci Rep, 2014. 4: p. 7399
    [PMID:25492247]
  3. Ortega-Amaro MA, et al.
    Overexpression of AtGRDP2, a novel glycine-rich domain protein, accelerates plant growth and improves stress tolerance.
    Front Plant Sci, 2014. 5: p. 782
    [PMID:25653657]
  4. Wang Y, et al.
    MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development.
    Plant Physiol., 2015. 168(3): p. 984-99
    [PMID:25941314]
  5. Barik S, et al.
    Coevolution Pattern and Functional Conservation or Divergence of miR167s and their targets across Diverse Plant Species.
    Sci Rep, 2015. 5: p. 14611
    [PMID:26459056]
  6. Pashkovskiy PP,Kartashov AV,Zlobin IE,Pogosyan SI,Kuznetsov VV
    Blue light alters miR167 expression and microRNA-targeted auxin response factor genes in Arabidopsis thaliana plants.
    Plant Physiol. Biochem., 2016. 104: p. 146-54
    [PMID:27031426]
  7. Mlotshwa S,Pruss GJ,MacArthur JL,Reed JW,Vance V
    Developmental Defects Mediated by the P1/HC-Pro Potyviral Silencing Suppressor Are Not Due to Misregulation of AUXIN RESPONSE FACTOR 8.
    Plant Physiol., 2016. 172(3): p. 1853-1861
    [PMID:27688620]
  8. Wójcikowska B,Gaj MD
    Expression profiling of AUXIN RESPONSE FACTOR genes during somatic embryogenesis induction in Arabidopsis.
    Plant Cell Rep., 2017. 36(6): p. 843-858
    [PMID:28255787]
  9. Zhang GZ, et al.
    Ectopic expression of UGT84A2 delayed flowering by indole-3-butyric acid-mediated transcriptional repression of ARF6 and ARF8 genes in Arabidopsis.
    Plant Cell Rep., 2017. 36(12): p. 1995-2006
    [PMID:29027578]
  10. Zheng K, et al.
    Involvement of PACLOBUTRAZOL RESISTANCE6/KIDARI, an Atypical bHLH Transcription Factor, in Auxin Responses in Arabidopsis.
    Front Plant Sci, 2017. 8: p. 1813
    [PMID:29114256]
  11. Liu Z, et al.
    ARF2-ARF4 and ARF5 are Essential for Female and Male Gametophyte Development in Arabidopsis.
    Plant Cell Physiol., 2018. 59(1): p. 179-189
    [PMID:29145642]
  12. Ghelli R, et al.
    A Newly Identified Flower-Specific Splice Variant of AUXIN RESPONSE FACTOR8 Regulates Stamen Elongation and Endothecium Lignification in Arabidopsis.
    Plant Cell, 2018. 30(3): p. 620-637
    [PMID:29514943]