PlantTFDB
PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
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(e.g., LFY)
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID XP_016514895.1
Organism
Nicotiana tabacum
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana
Family ARF
Protein Properties Length: 845aa    MW: 93973.3 Da    PI: 6.9129
Description ARF family protein
Gene Model
Gene Model ID Type Source Coding Sequence
XP_016514895.1genomeNCBIView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1B375.65.8e-24146241199
                     EEEE-..-HHHHTT-EE--HHH.HTT---..--SEEEEEETTS-EEEEEE..EEETTEEEE-TTHHHHHHHHT--TT-EEEEEE-SSSEE..EEE CS
              B3   1 ffkvltpsdvlksgrlvlpkkfaeehggkkeesktltledesgrsWevkliyrkksgryvltkGWkeFvkangLkegDfvvFkldgrsefelvvk 95 
                     f+k+lt sd++++g +++ +++a++++ ++++ ++l+ +d++g++W++++i+r++++r++l++GW+ Fv++++L +gD ++F   + +++el+v+
  XP_016514895.1 146 FCKTLTASDTSTHGGFSVLRRHADDMSRQPPT-QELVAKDLHGNEWRFRHIFRGQPRRHLLQSGWSVFVSSKRLVAGDAFIFL--RGENGELRVG 237
                     99*********************999777666.49************************************************..449999**** PP

                     EE-S CS
              B3  96 vfrk 99 
                     v+r+
  XP_016514895.1 238 VRRA 241
                     *996 PP
2Auxin_resp121.37.5e-40266348183
      Auxin_resp   1 aahaastksvFevvYnPrastseFvvkvekvekalkvkvsvGmRfkmafetedsserrlsGtvvgvsdldpvrWpnSkWrsLk 83 
                     a+ha++tk++F+v+Y+Pr+s++eF+v+++++++++k+++s+GmRfkm+fe+e+++e+r++Gt+vg++d+dp rWp+SkWr+Lk
  XP_016514895.1 266 AWHAIQTKTMFTVYYKPRTSPTEFIVPYDQYMESVKNNYSIGMRFKMRFEGEEAPEQRFTGTIVGTEDSDPKRWPESKWRCLK 348
                     89*******************************************************************************96 PP
Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:2.40.330.102.6E-36140255IPR015300DNA-binding pseudobarrel domain
SuperFamilySSF1019361.27E-35142269IPR015300DNA-binding pseudobarrel domain
CDDcd100171.77E-21144240No hitNo description
PfamPF023622.6E-22146241IPR003340B3 DNA binding domain
PROSITE profilePS5086312.506146242IPR003340B3 DNA binding domain
SMARTSM010198.3E-21146242IPR003340B3 DNA binding domain
PfamPF065073.7E-37266348IPR010525Auxin response factor
PfamPF023091.5E-10709804IPR033389AUX/IAA domain
SuperFamilySSF542771.47E-12714796No hitNo description
PROSITE profilePS5174525.301719803IPR000270PB1 domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009725Biological Processresponse to hormone
GO:0005634Cellular Componentnucleus
GO:0003677Molecular FunctionDNA binding
GO:0005515Molecular Functionprotein binding
Sequence ? help Back to Top
Protein Sequence    Length: 845 aa     Download sequence    Send to blast
MSTSEVSIKG YSEPIDGSRP VSETVRSAPV VGKVDAETAL YTELWRACAG PLVTVPREGE  60
LVYYFPQGHI EQVEASTNQV ADQQMPLYNL PSKILCRVVN VLLKAEPDTD EVFAQVTLMP  120
EPNQDENTVK KESMRPPPPR FHVHSFCKTL TASDTSTHGG FSVLRRHADD MSRQPPTQEL  180
VAKDLHGNEW RFRHIFRGQP RRHLLQSGWS VFVSSKRLVA GDAFIFLRGE NGELRVGVRR  240
AMRQQGNAPS SVISSHSMHL GVLATAWHAI QTKTMFTVYY KPRTSPTEFI VPYDQYMESV  300
KNNYSIGMRF KMRFEGEEAP EQRFTGTIVG TEDSDPKRWP ESKWRCLKVR WDENSTIPRP  360
DQVSPWKIEP ALSPPALNAP PIARPKRPRS SILPSSPDSS VLTREGSSRI TADPSQASGF  420
SRVLQGQELS TLRGGFAESN DLDVSEKPMI WPPSVDDEKI DIHSASKRYL SDKWLPLGRP  480
ESSLTDLLSG FGVQNNSSHG FCLPSADKTA FGASLVKRQA QDQEKDFSLL GKPWSLLSSG  540
LSLNLMDSGS KAPGQGGDTP YQMQGDARYS GFGEFSVLPG HRVDNQQGSW FMPQSVSPYF  600
QLSSHSRELR HKPTVVQKPE AVKPKEGNCK LFGIPLTSNV CTDPVMTRKR SLMDSASNMN  660
IGINSHQSPA TDSDQRSEQS KGSKVLDDGI TANDQDKQFH TSHLAARDRD AKGHSSSTRS  720
CTKVHKQGTA LGRSVDLAKF NNYDELIAEL DQLFDFNGEL KARSKSWLVV YTDDEGDMML  780
VGDDPWQEFC GMVRKIFIYT KDEVQRMNPG TLNSKGEDTS SVAEGSEAKE VKSLQHPSES  840
GQEES
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
4ldv_A1e-1663937018354Auxin response factor 1
4ldw_A1e-1663937018354Auxin response factor 1
4ldw_B1e-1663937018354Auxin response factor 1
Search in ModeBase
Functional Description ? help Back to Top
Source Description
UniProtAuxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs) (By similarity). Could act as transcriptional activator or repressor (PubMed:26959229, PubMed:26716451, PubMed:27645097, PubMed:28167023). Involved in the control of fruit ripening process (PubMed:26959229, PubMed:26716451, PubMed:28167023). Regulates expression of a number of ripening regulators, transcription factors, and ethylene biosynthesis and signaling components (PubMed:26959229, PubMed:26716451). May act as a transcriptional repressor of auxin-responsive genes (PubMed:26716451). Regulates vegetative growth, lateral root formation and flower organ senescence, possibly partially by regulating gene expression of auxin and ethylene response factor (ERF) genes (PubMed:28167023). Plays a negative role in axillary shoot meristem formation (PubMed:27645097). {ECO:0000255|RuleBase:RU004561, ECO:0000269|PubMed:26716451, ECO:0000269|PubMed:26959229, ECO:0000269|PubMed:27645097, ECO:0000269|PubMed:28167023}.
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: By tomato fruit ripening. Expression in tomato fruits is up-regulated by exogenous application of ethylene and down-regulated by inhibition of ethylene receptors with 1-methylcyclopropene (1-MCP) (PubMed:26959229, PubMed:26716451). Up-regulated by plant hormone abscisic acid (ABA) in tomato fruits (PubMed:26959229). Expression is not up-regulated by plant hormone auxin in tomato fruits (PubMed:26716451). Up-regulated by auxin and gibberellic acid (GA), and down-regulated by ethylene in seedlings (PubMed:28167023). Down-regulated during decapitation-induced axillary shoot development and by excision of immature leaves. Down-regulation is inhibited by the application of auxin on the cut surface (PubMed:27645097). {ECO:0000269|PubMed:26716451, ECO:0000269|PubMed:26959229, ECO:0000269|PubMed:27645097, ECO:0000269|PubMed:28167023}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieve-
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankDQ3402550.0DQ340255.1 Lycopersicon esculentum auxin response factor 2 (ARF2) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqXP_016514895.10.0PREDICTED: auxin response factor 2-like isoform X1
SwissprotQ2LAJ30.0ARF2A_SOLLC; Auxin response factor 2A
TrEMBLA0A1S4DNB80.0A0A1S4DNB8_TOBAC; Auxin response factor
STRINGXP_009625131.10.0(Nicotiana tomentosiformis)
Best hit in Arabidopsis thaliana ? help Back to Top
Hit ID E-value Description
AT5G62000.30.0auxin response factor 2
Publications ? help Back to Top
  1. Tomato Genome Consortium
    The tomato genome sequence provides insights into fleshy fruit evolution.
    Nature, 2012. 485(7400): p. 635-41
    [PMID:22660326]
  2. Breitel DA, et al.
    AUXIN RESPONSE FACTOR 2 Intersects Hormonal Signals in the Regulation of Tomato Fruit Ripening.
    PLoS Genet., 2016. 12(3): p. e1005903
    [PMID:26959229]
  3. Xu T, et al.
    SlARF2a plays a negative role in mediating axillary shoot formation.
    Sci Rep, 2016. 6: p. 33728
    [PMID:27645097]
  4. Ren Z,Liu R,Gu W,Dong X
    The Solanum lycopersicum auxin response factor SlARF2 participates in regulating lateral root formation and flower organ senescence.
    Plant Sci., 2017. 256: p. 103-111
    [PMID:28167023]